Cellular automata approach for the dynamics of HIV infection under antiretroviral therapies: The role of the virus diffusion

We study a cellular automata model to test the timing of antiretroviral therapy strategies for the dynamics of infection with human immunodeficiency virus (HIV). We focus on the role of virus diffusion when its population is included in previous cellular automata model that describes the dynamics of the lymphocytes cells population during infection. This inclusion allows us to consider the spread of infection by the virus-cell interaction, beyond that which occurs by cell–cell contagion. The results show an acceleration of the infectious process in the absence of treatment, but show better efficiency in reducing the risk of the onset of AIDS when combined antiretroviral therapies are used even with drugs of low effectiveness. Comparison of results with clinical data supports the conclusions of this study.     

广西仙湖实验动物养殖场猕猴病毒感染的监控

本文报道广西仙湖实验动物养殖场三代猕猴病毒感染八年来的监控结果，猴Ｂ病毒、巨细胞病毒由亲代１００％的感染率到子一代和子二代变为阴性。猴痘病毒、麻疹病毒、爱滋病毒、反转录Ｄ型病毒、Ｔ淋巴细胞趋向病毒Ｉ型、Ｖ型、甲、乙肝病毒感染保持阴性。对养殖管理系统的工作作了探讨。     

新疆常见植物杀蚜效果的初筛试验

对16种新疆常见的植物进行了室内杀蚜效果的初步筛选试验，结果表明：杀蚜效果最好的是辣椒，其次是马齿苋，其它依次为苦豆子、艾蒿、万年青和扁蓄；处理48h后，校正死亡率最低为62.5%，最高可达91.7%。并对杀蚜植物进一步研究应注意的问题提出了建议。     

SARS冠状病毒基因片段变异分析

采用基因序列和生物信息分析法，研究了SARS-CoV复制酶基因中4个片段。结果发现，片段Ⅰ与已报道的SARS冠状病毒ZJ01有2个位点不同，片段Ⅲ与CUHK-W1、CUHK-Su1O、HKU-39849和Hong-Kong各有1个位点的差别；片段Ⅳ与GZ01间有1个位点的变化。同时对已公布的17个全基因组序列进行比对分析。可找到共137个变异位点，其中仅出现1次的变异位点119个，间约信息位点18个。     

824例儿童腹泻病原分析

目的：探讨儿童腹泻的病原检验结果,以此为基础进行儿童腹泻的病原分析并提出治疗意见.方法 ：对内蒙古民族大学附属医院824例腹泻患儿的病原检验结果进行回顾性分析,总结常见病原体.结果：所有患儿中,病毒感染率为45.75%,主要感染病毒为轮状病毒和腺病毒;细菌感染率为12.38%,主要感染细菌为沙门菌和志贺杆菌.结论：导致儿童腹泻的主要病原为病毒,且以轮状病毒感染为主,次要病原为细菌感染,因此,临床治疗时需要根据检验所得出的患者的实际情况进行治疗.     

仙台病毒抗原的制备及初步应用

目的纯化和制备仙台病毒(Sendai Virus,SeV)抗原,初步应用于间接酶联免疫吸附试验(ELISA)及免疫印迹(Western-blot)试验对SeV抗体进行检测。方法通过鸡胚尿囊腔及BHK-21细胞增殖法培养增殖SeV,低温冻融去除细胞碎片后,用差速离心及超声破碎法纯化并制备SeV抗原,以SDS-PAGE电泳进行纯度鉴定;优化确定间接SeV-ELISA抗原的最佳包被浓度,对比检测间接免疫荧光试验(IFA)初筛小鼠SeV阳性血清,同时用Western-blot检测法进行特异性验证。结果用BHK-21细胞培养增殖的SeV,病毒滴度HA为1∶128,SeV抗原的电泳纯度达80%;确定SeV-ELISA抗原最佳包被浓度为2.5μg/mL时,测得小鼠SeV抗体ELISA与IFA的阳性符合率为100%,并由Western-blot检测法得到了证实。结论通过BHK-21细胞增殖和差速离心及超声破碎法制备的SeV抗原,可用于ELISA及Western-blot法对小鼠SeV抗体的检测,进而验证了SeV具有较强的抗原特异性及蛋白活性。     

Reduced surface area chromatography for flow-through purification of viruses and virus like particles

A method for flow-through purification of viruses and virus like nano-particles using a combination of binding and size-exclusion chromatography was developed. This technique relies on minimizing the external surface area per unit volume available for virus binding by increasing the mean diameter of the beads used in the column. At the same time the impurity binding capacity of the column is maximized by utilizing beads with multiple functionalities of the optimum size. Purification of different types of viruses and virus-like-particles could be achieved using this technique. Flow-through purification of influenza virus using this technique yielded virus recoveries greater than 70-80% coupled with impurity removal greater than 80%. Finally an approach to optimize and facilitate process development using this technology is presented. Since the impurity binding occurs via a non-specific mechanism and virus recovery is achieved through reduced surface area, the technique is not limited to specific types of viruses and offers the potential as a universal purification tool.     

Parallel differential mobility analysis for electrostatic characterization and manipulation of nanoparticles and viruses

The electrophoretic mobility of charged, airborne nanoparticles (NPs) or macromolecules and their specific complexes opens new avenues for their analysis and handling. The newly developed parallel differential mobility analyzer in combination with an electrostatic particle sampler enables not only the characterization of bio-NPs, but even their sampling while preserving their bioactivity (e.g., the enzyme activity of galactosidase). Precondition for the applicability of this technique is a well-defined charging status of the NPs in question. This charge conditioning can be achieved by means of a radioactive source, Po-210, even if the yield in terms of charged particles is low for sub-20-nm particles and the aging of the source influences the size spectra measured. Nevertheless, this technique enables size-defined sampling and enrichment, combined with real-time measurement of the size of both NPs and viruses. Furthermore, it allows determination of the number of attached biospecific antibodies, thereby providing information about the surface coverage of viruses by antibodies.     

压回法阶段压井数学模型与模拟分析

 根据压回法压井过程中气体压缩规律和气液两相流特性,提出了压回法压井过程可分为3 个阶段,并建立了考虑气体滑脱的阶段压井数学模型,用于压回法作业井底压力和套压变化规律的定量计算。压井参数敏感性模拟分析表明,压井排量越大,压井持续时间越短,产生的井底压力和套压越大;地层渗透率和渗透性地层厚度对压井第一阶段的井底压力和套压影响不大,但当高渗透或高厚度地层进入压井第二、第三阶段,随渗透率或地层厚度增加,压井产生的井底压力和套压减小,且降幅减缓。通过分析压井过程中井底压力和套压变化规律,绘制了压回法典型压井曲线模型。预测结果与实测数据对比表明：该数学模型具有较高的预测精度,能够为溢流压井方法的选择和压回法施工设计提供理论依据与指导。     

复肝灵抗鸭体内乙型肝炎病毒的实验研究

目的：观察复肝灵抗鸭乙型肝炎病毒（DHBV）的作用,为复肝灵的临床应用提供实验依据。方法：采用一日龄北京鸭30只,静脉注射雏鸭乙型肝炎病毒阳性血清建立鸭乙型肝炎病毒模型,然后随机分为复肝灵组、病毒对照组和拉米夫定组。复肝灵组分为0．5、1．0、2．0g／kg三个剂量组。给鸭口服复肝灵,1天2次,给药10天。然后分别在给药前、给药后5天、10天及停药3天后取血,测定鸭血清中DHBV—DNA水平并计算每组鸭用药后不同时间（T5、T10）和停药第3天（P3）血清DHBV-DNA的抑制率（％）,观察鸭血清鸭乙型肝炎病毒DNA（DHBV—DNA）的影响。病毒对照组以生理盐水代替药物;拉米夫定组给予口服拉米夫定。结果：复肝灵2．0g／kg组,给药后第5天,第10天鸭血清DHBV—DNA显著下降（P〈0．01）,停药后3天鸭血清DHBV—DNA下降明显（P〈0．05）,鸭血清DHBV—DNA水平抑制率则有显著升高;1．0g／kg组,给药后第10天和停药后3天鸭血清DHBV—DNA明显下降,鸭血清DHBV—DNA水平抑制率则有显著升高,与对照组差异显著（P〈0．01）,与拉米夫定组则无明显区别：0．5g／kg组则无明显变化。结论：大剂量组、中等剂量的复肝灵组在鸭体内有抗鸭乙型肝炎病毒作用。