采用正交设计和响应曲面设计法优化芦苇随机扩增多态DNA体系

采用正交设计和响应曲面设计(RSD)优化适合于芦苇[Phragmites australis (Cav.)Trin.ex steud]增多态DNA(RAPD)的反应体系,结果确定芦苇RAPD反应体系的最佳方案为：在PCR扩增程序,冷启动,94℃变性1min,36℃复性90s,72℃延伸2min,40个循环;72℃延伸7min,4℃保存;25μl反应体系包括模板DNA 60ng、三磷酸脱氧核苷酸(dNTP)7．5mmol、Taq酶1．5u、引物5pmol。     

瑶湖流域非点源氮、磷负荷估算及其控制对策

利用ArcGIS10.0软件,划分了瑶湖流域的范围,并提取了土地利用类型及其面积,在流域调查的基础上,参考国内外相关研究方法,按照农村与城镇生活污染(A类污染)、畜禽养殖污染(B类污染)、村镇与农业径流污染(C类污染)和水产养殖污染(D类污染)等4种污染来源,估算了瑶湖流域的非点源氮、磷污染负荷。结果表明:入湖氮、磷总负荷分别为346.3 t/a和102.8 t/a;B类污染是氮和磷污染的主要来源,其污染负荷占总负荷的比例分别为73.1%和85.3%;南昌县对B类污染的TN和TP贡献率大于青山湖区;南昌县对A类污染的TN和TP贡献率稍小于青山湖区;对于C类污染,TN贡献率大小为:水田村镇水浇地,TP贡献率大小为:村镇水田水浇地。在此分析基础上,提出了非点源N、P污染负荷控制对策。这为瑶湖流域水污染控制提供了理论依据和数据支撑,同时也为政府制定区域社会经济发展规划提供了决策依据。     

关于PAL电视信号优质化处理的研讨

本文提出一种可克服漏检问题的改进的运动检测方法。该方法用于Y／C分离和倍场处理,可提高电视图像的质量。     

方格星虫滩涂养殖对沉积物TOC和TN垂直分布的影响

为探讨方格星虫(Sipunculus nudus)滩涂养殖对沉积物环境的影响,采用滩涂围隔法研究方格星虫不同养殖密度(0、5、10、15、20 ind./m²)条件下围隔中沉积物总有机碳(TOC)和总氮(TN)含量的季节变化。结果显示,10 ind./m²养殖密度处理下的方格星虫成活率和规格最高,分别为(61.1±36.1)%和(5.21±0.69) g/ind.,但不同处理间差异不显著(P>0.05)。方格星虫不同密度养殖过程中,滩涂沉积物的TOC含量基本呈随着深度的增加而升高的趋势,且不同深度间TOC含量存在显著差异(P<0.05);而TN含量垂直分布比较均匀,说明方格星虫生物扰动会导致有机质向下层迁移。整体上来看,所有养殖密度处理围隔中滩涂沉积物TOC和TN平均含量分别呈现“V”型和“∧”型的季节变化规律。15 ind./m²和20 ind./m²养殖密度处理围隔中滩涂沉积物TOC的全年平均含量低于空白对照;所有养殖密度处理围隔中滩涂沉积物TN的全年平均含量均高于空白对照。研究结果...     

TN接地系统中接地故障保护

针对电气故障引发的火灾居首位,必须采取有效措施来限制接地故障电流、接地故障电压,并给出解决方案.     

南湖疏浚后底泥中氮、磷分布规律研究

为了解疏浚后南湖底泥中氮磷等营养元素的分布规律,选取湖泊周围及中心6处取样。通过实验测定底泥中总磷、总氮含量,发现：（1）在疏浚后,湖泊底泥中氮磷含量仍然很高,比大型浅水湖底泥中含量要高得多;（2）从水平分布来看,湖泊四周的氮、磷营养元素含量比湖中心处要高,且西部自北向南形成较高区域,、整个湖泊各监测点处总氮含量要比总磷含量高得多;（3）从垂直分布看,湖泊底泥中营养元素在表层下约100cm处达到最大值,总氮、总磷含量随深度的变化关系并不单一的增减。底泥疏浚时,需考虑合理的疏浚深度。     

Distribution characteristics of soil organic matter and total nitrogen on the Yajiageng vertical belt,Gongga Mountain around the Dadu River banks

Distribution characteristics of soil organic matter（SOM） and total nitrogen（TN） were studied in different plant communities of the Yajiageng vertical belt in Gongga Mountain around the Dadu River banks. The results show： （1） the contents of SOM and TN of the plant communities gradually decreased with the following order： subalpine coniferous forest （3 027 m）, subalpine meadow （3 873 m）, coniferous broadleaved mixed forest（2 737 m）, subalpine shrub（3 565 m） and treeline（3 564 m）. （2） With soil profile depth increasing, the contents of SOM and TN gradually decreased. For different vegetation types, the contents of SOM and TN in sub-alpine coniferous forest were higher than that of other vegetational types. （3）The ratio of the content of carbon to the content of total nitrogen （Cc/CTN）WaS 13.5-27.6, which was relatively lower than the appropriate Cc/CTN of 25-30, and indicated that the soil in favor of the organic matter decomposed and nutrients released. Cc/CTN in the soil had no correlation with sea level altitude. However, its distribution in the soil x, aried with different vegetation types. （4） Nitrogen in SOM existed mainly in the form of organic nitrogen, and Cc/CTN in the soil was not obvious correlated with SOM and TN.     

Ionic effects on electro-optics and residual direct current voltages of twisted nematic liquid crystal cells

By using electro-optical and dielectric absorption [1–4] measurements, we report our investigations of ionic effects on electro-optics and residual direct current voltages (VrDCs) of two polyimide (PI)-aligned twisted nematic (TN) cells with same liquid crystal mixture but different PI-alignment materials. We have also carried out new experimental methods to find out that the observed VrDCs were caused by LC-PI-interfacial trapped ions generated and transported from the LC medium for one TN cell, and from the PI layers for the other TN cell. Our measured VrDCs indicated that the former had two different exponential-decay rates similar to the published results by M. Mizusaki et al. [2] but the latter had only a single exponential-decay rate.     

TN液晶稀释剂4－（4＇－正丙基环己基）苯甲（乙）醚的合成

ＴＮ液晶稀释剂４－（４＇－正丙基环己基）苯甲（乙）醚的合成马汝建，程旻，荣国斌（华东理工大学化学系上海２００２３７）关键词　ＴＮ液晶，稀释剂，合成扭曲向列（ＴＮ）型液晶材料的粘度越低，响应速度越快［１］．为降低液晶材料的粘度，改善其性能，常在ＴＮ液晶...