薄层色谱法鉴别车用润滑油

采用薄层色谱法,以正己烷∶二氯甲烷(8∶2,V/V)为展开剂对64种不同型号,适用于不同车型的润滑油进行了种类鉴别与区分,并实现了水泥与沥青路面上的油脂与原样品的比对.     

薄层色谱跟踪罗沙替丁醋酸酯的合成展开剂的选择

用薄层色谱(TLC)跟踪监测合成罗沙替丁醋酸酯过程中每一步反应,结果显示:1-3步的合成反应监测过程采用的展开剂都是甲醇∶氯仿∶氨水(1∶4:0,5;V/V/V);第4步合成反应监测过程中,当展开剂石油醚∶氯仿:丙酮:乙醇的配比为23.5∶ 12:2∶2.5(V/V/V/V)时,原料中间体及终产物的Rf值可控制在0.2...     

Common methods for the chiral determination of amphetamine and related compounds I. Gas, liquid and thin-layer chromatography

This article reviews the most common, useful methods for the chiral determination of amphetamine (AM) and AM-derived designer drugs in different of matrix, including blood, hair, urine, medicaments or standard solutions, taking into consideration articles published in the past 15 years. We consider chromatographic methods (e.g., gas, liquid, high-performance liquid, and thin layer). We describe several types of chiral derivatization reagent, mobile-phase additive and chiral stationary phase commonly used in the chromatographic methods. Tables summarize basic information about conditions (e.g., type of column and mobile phase), detection mode and reference data for each procedure.     

Rapid biosynthesis of silver nanoparticles from Bacillus megaterium (NCIM 2326) and their antibacterial activity on multi drug resistant clinical pathogens

The notion to fight against multi drug resistant pathogens is a great deal in the field of nanomedicine. The identifiable antimicrobial action of metal bionanoparticles on many microorganisms is reported earlier. As silver bionanoparticles (Ag-BNPs) are known to have efficient antibacterial properties they are synthesized in ecofriendly and biocompatible way. The present study is focused on the extracellular biosynthesis of highly stable Ag-BNPs from bacterial strain Bacillus megaterium (NCIM 2326) by bio-reduction of silver ion using the culture supernatant, and to determine the antibacterial efficacy on multi drug resistant clinical pathogens such as Streptococcus pneumoniae and Salmonella typhi. The biosynthesis process is rapid and Ag-BNPs are formed within few minutes if AgNO(3) comes to contact with cell filtrate. Furthermore the synthesized Ag-BNPs are characterized by UV-vis spectroscopy, Atomic Force Microscopy (AFM), Thin Layer Chromatography (TLC) and Fourier Transform Infrared Spectroscopy (FTIR).     

蒲地蓝消炎片质量控制方法研究

目的：建立蒲地蓝消炎片的质量控制方法。方法：采用薄层色谱法定性鉴别,展开剂为醋酸乙酯-丙酮-甲酸-水（13：3．5：1：1．5）,用2％FeCl3乙醇溶液显色。采用反相高效液相色谱法测定蒲地蓝消炎片中黄芩苷的含量,色谱条件为Agilent ZORBAXSB C18色谱柱（4．6mm×150mm,5μm）,流动相为甲醇-0．2％磷酸水溶液（42：58）,检测波长为278nm,流速1．0ml／min,柱温30℃。结果：薄层色谱中供试品与对照品在相应位置上具有相同颜色的斑点。黄芩苷在0．2～2．0ng范围呈良好的线性关系,r=0．9998,回收率为99．65％,RSD为1．60％。结论：本法操作简便快速,分离效果好,稳定性高,重现性好,可全面控制蒲地蓝消炎片的质量。     

Comparison of high performance TLC and HPLC for separation and quantification of chlorogenic acid in green coffee bean extracts

Two chromatographic methods, high-performance TLC (HPTLC) and HPLC, were developed and used for separation and quantitative determination of chlorogenic acid in green coffee bean extracts. For HPTLC silica gel Kieselgel 60 F 254 plates with ethyl acetate/dichlormethane/formic acid/acetic acid/water (100:25:10:10:11, v/v/v/v/v) as mobile phase were used. Densitometric determination of chlorogenic acid by HPTLC was performed at 330 nm. A gradient RP HPLC method was carried out at 330 nm. All necessary validation tests for both methods were developed for their comparison. There were no statistically significant differences between HPLC and HPTLC for quantitative determination of chlorogenic acid according to the test of equality of the means.     

复方儿茶生肌油中大黄薄层色谱鉴别方法的研究

采用薄层色谱鉴别法（TLC）对复方儿茶生肌油中大黄成分进行鉴别,结果表明,在制备供试品溶液时,采用5％氢氧化钠溶液提取样品中的大黄,比用甲醇分离效果更好,重现性好,可作为该制剂拟定质量标准的鉴别方法。     

Current Response of Cytochrome C Promoted by Dodecyl Benzene Sodium Sulfonate

Abstract

A simple TLC separation and semi-quantitative visual determination method was used to analyse the volatile phenols in the waste water of the petroleum industry and the grain irrigated by the waste water. The phenol in the rice irrigated by the water that contained phenol for artificial preparation was also studied. A preliminary conclusion was obtained, that the grain could absorb volatile phenols from irrigation water.     

Direct Thin – Layer Chromatographic Separation of Enantiomers of Six selected Amino Acids Using 2-O-[(R)-2-Hydroxypropyl]-β-CD as a Mobile Phase Additive

Abstract

Compared with β-Cyclodextrin (β-CD), 2-O-[_(R)-2-hydroxypropyl]-β-CD (A) is more sensitive for chiral recognition. When (A) was used as the chiral mobile phase additive, some racemic amino acids were directly separated on silica gel TLC for the first time.     

Rapid and Highly Sensitive HPLC and TLC Methods for Quantitation of Amlodipine Besilate and Valsartan in Bulk Powder and in Pharmaceutical Dosage Forms and in Human Plasma

Two simple, sensitive, and specific high-performance liquid chromatography and thin-layer chromatography methods were developed for the simultaneous estimation of Amlodipine besilate (AM) and Valsartan (VL). Separation by HPLC was achieved using a xTerra C18 column and methanol /acetonitrile /water/ 0.05% triethylamine in a ratio 40:20:30:10 by volume as mobile phase, pH was adjusted to 3 ± 0.1 with o-phosphoric acid. The flow rate was 1.2 mL min^?1. The linearity range was 0.2 to 2 µg mL^?1 for amlodipine besilate and 0.4 to 4 µg mL^?1 for Valsartan with a mean percentage recovery of 99.59 ± 0.523% and 100.61 ± 0.400% for amlodipine besilate and valsartan, respectively. The TLC method used silica gel 60 F₂₅₄ plates; the optimized mobile phase was ethyl acetate/ methanol / ammonium hydroxide (55:45:5 by volume). Quantitatively, the spots were scanned densitometrically at 237 nm. The range was 0.5–4.0 µg spot^?1 for amlodipine besilate and 2.0–12.0 µg spot^?1 for valsartan. The mean percentages recovery was 99.80 ± 0.451% and 100.61 ± 0.363% for amlodipine besilate and valsartan, respectively. The HPLC method was found to be simple, selective, precise, and reproducible for the estimation of both drugs from spiked human plasma.