全文获取类型
收费全文 | 562篇 |
免费 | 22篇 |
国内免费 | 15篇 |
专业分类
化学 | 392篇 |
力学 | 1篇 |
综合类 | 3篇 |
物理学 | 22篇 |
综合类 | 181篇 |
出版年
2023年 | 2篇 |
2022年 | 9篇 |
2021年 | 2篇 |
2020年 | 5篇 |
2019年 | 9篇 |
2018年 | 13篇 |
2017年 | 12篇 |
2016年 | 9篇 |
2015年 | 13篇 |
2014年 | 14篇 |
2013年 | 9篇 |
2012年 | 42篇 |
2011年 | 21篇 |
2010年 | 19篇 |
2009年 | 25篇 |
2008年 | 24篇 |
2007年 | 37篇 |
2006年 | 31篇 |
2005年 | 22篇 |
2004年 | 17篇 |
2003年 | 19篇 |
2002年 | 14篇 |
2001年 | 16篇 |
2000年 | 12篇 |
1999年 | 12篇 |
1998年 | 6篇 |
1997年 | 8篇 |
1996年 | 4篇 |
1995年 | 4篇 |
1994年 | 8篇 |
1993年 | 4篇 |
1992年 | 4篇 |
1991年 | 2篇 |
1990年 | 2篇 |
1989年 | 13篇 |
1988年 | 7篇 |
1987年 | 4篇 |
1986年 | 6篇 |
1985年 | 13篇 |
1984年 | 18篇 |
1983年 | 8篇 |
1982年 | 12篇 |
1981年 | 18篇 |
1980年 | 15篇 |
1979年 | 22篇 |
1978年 | 10篇 |
1975年 | 3篇 |
排序方式: 共有599条查询结果,搜索用时 15 毫秒
51.
52.
Gorchein A 《Biomedical chromatography : BMC》2003,17(8):526-529
Reversed-phase TLC systems using ion-pairing reagents are described for the separation without prior derivatization of the porphyrins of rat Harderian gland. Porphyrins with R(f) values greater than protoporphyrin but smaller than coproporphyrin, representing harderoporphyrin, were labile to 1 m NaOH, which converted them to protoporphyrin, inconsistent with the established literature that harderoporphyrin is a tricarboxylic acid porphyrin. It was confirmed by HPLC/electrospray ionization MS that this 'harderoporphyrin' fraction consists of the recently characterized glycoconjugate, protoporphyrin-1-O-acyl beta-xyloside, with trace amounts of protoporphyrin-1-O-acyl beta-glucoside. 相似文献
53.
荧光试剂衍生化液相色谱分析生物检材中的氟乙酸钠 总被引:2,自引:0,他引:2
建立了使用新的荧光衍生试剂9-氯甲基蒽(CA)衍生化生物检材中的氟乙酸钠,并通过薄层色谱和荧光检测的高效液相色谱(HPLC)分析的方法。合成得到氟乙酸钠的荧光衍生物——氟乙酸-9-亚甲基蒽酯(MA-MFA),纯度达到98%以上,通过红外光谱、核磁共振、元素分析和质谱对其进行结构表征。薄层色谱中展开剂为石油醚/乙酸乙酯(体积比为95∶5),检测限为5×10-8g(TLC)。高效液相色谱用AgilentHypersilODS反相色谱柱,以乙腈/水(体积比为85∶15)为流动相,荧光检测波长λex为256 nm,λem为412 nm。方法在2.5×10-9~125×10-9mol/mL浓度范围内呈良好的线性关系,线性相关系数为0.9988,生物样品空白添加实验的回收率为83%~90%,最低检测限为2×10-10g(S/N=3),日内、日间RSD均小于4%。该方法用于中毒死亡者的血液样品及其他检材的测定,效果良好。 相似文献
54.
55.
Jacek Achrem‐Achremowicz Elżbieta Kępczyńska Marek Żylewski Zbigniew Janeczko 《Biomedical chromatography : BMC》2010,24(3):261-267
A series of superlipophilic or highly lipophilic semisynthetic betulin derivatives was prepared and their relative lipophilicity was measured by reversed‐phase thin‐layer chromatography (RP‐TLC) at different pH values using 1,4‐dioxane–acetate buffer mixtures as mobile phases. Cholesterol, 17β‐estradiol and pure betulin were used as the reference compounds. Linear relationships were found between RM values and 1,4‐dioxane concentrations in the mobile phases. LogP values were also calculated with computer programs ACD/LogP (ChemSketch 11.0, Advanced Chemistry Development Inc.) and ClogP (Daylight Chemical Information Systems Inc.). The empirical and theoretical data were compared, and the RM0 values correlated well with logP. Two of the synthesized betulin derivatives are reported for the first time. Copyright © 2009 John Wiley & Sons, Ltd. 相似文献
56.
构建重组菌株E.coliBL21(DE3)转pET23a-tyrB,IPTG诱导其大量表达,然后依次通过盐析、离子交换层析、疏水层析、凝胶过滤层析获得纯酶,运用薄层层析技术(TLC)检测,结果表明纯化的TyrAT转氨酶对自身底物酪氨酸在37℃和50℃具有较高活性.分别加入非天然氨基酸L-正缬氨酸、L-新戊基甘氨酸、L-叔亮氨酸、D-正亮氨酸作为底物,检测到TyrAT转氨酶对L-叔亮氨酸在50℃下有活性,对D-正亮氨酸在37℃和50℃均有催化活性,且50℃下效果更好. 相似文献
57.
A new, fast and convenient method based on coupled thin-layer chromatography (TLC) and Fourier transform-infrared (FT-IR) microscopy is developed to separate, detect and identify the additives in polymers. After the TLC development, the analytes were transferred on to a barium fluoride (BaF2) salt plate via a special capillary technique and analysed by FT-IR microscopy. The additives used for stabilization of polypropylene and the plasticisers used for poly(vinyl chloride) were analysed as examples to illustrate this technique. The overall time taken for the experiment including transferring three marked spots and then identifying them was about 20 min. An amount as small as 0.5 μg can be easily detected and identified. It was a very convenient and reliable method to separate and evaluate complex additives for polymers without the interference from TLC adsorbent, because of a special transferring and identifying method, which is suitable to FT-IR microscopy. 相似文献
58.
Summary Rf values of twenty therapeutic sulfonamides have been estimated in seven solvent systems. Based on these data a simple identification scheme was developed demanding only three solvent systems for complete separation.Quantification of corresponding fluorescamine derivatives at nanogram level was performed by the use of an TLC-scanner configured online with a microcomputer. Mathematical treatment of standard curves using the computer program Curve Fitter are discussed. Validity and reproducibility of results are compared with data obtained by HPLC and show good correlation. 相似文献
59.
本文采用薄层层析和簿层扫描定量法对庆大霉素C组分(包括发酵液)进行分离和测定.本 法快速、简便、取样量微,在2—10微克范围内溶液的峰面积积分值和庆大霉素的含量呈线性关 系。 相似文献
60.