Determination and Comparison of Phytoestrogens in Both Crude and Parched Flos Sophorae Immaturus by Capillary Electrophoresis with Electrochemical Detection

A method using high-performance capillary electrophoresis with electrochemical detection (CE-ED) has been developed for content comparison of phytoestrogens in both crude and parched Flos sophorae immaturus. Genistin, genistein, rutin, kaempferol and quercetin are important active constituents in this plant. The effects of several factors, such as acidity and concentration of running buffer, separation voltage, applied potential and injection time, were investigated to find the optimum conditions. Detection limits (S/N=3) ranged from 1.1 × 10^–7 to 2.8 × 10^–7gmL^–1 for all five analytes. This method was successfully used to analyse both crude and parched Flos sophorae immaturus after a relatively simple extraction procedure, and the assay results were satisfactory.     

On the absolute configuration of matrine: Crystal structure of matrin-1-IUM-trichlorozincate

The title compound crystallizes in the monoclinic space group P2₁ with a = 7.804(1)?, b = 13.597(2) ? c = 9.093(1) ?, β= 108.41(1)°, V = 915.5(2)?³, Z = 4, R = 0.0231, wR2 = 0.0475 (Siemens P4 diffractometer with MoK _α radiation, 3994 reflections collected at room temperature, 204 parameters refined). It is a zwitterionic complex (having Zn-O coordination bond of 2.003(2) ?) with a positive charge on the N1 atom of matrine molecule and a negative charge on the ZnCl₃ group. In the matrine molecule, N16 and H11 atoms are located on the opposite sides of the plane defined by C11, C15, C17 atoms and deviate from the plane by 0.075(3) ? and 0.839(3) ?, respectively, indicating a trans C-D ring junction. Therefore, the compound can be named trans-matrine. The D ring of matrinium demonstrates a distorted sofa conformation, while the other rings adopt chair forms. The A-B, A-C, B-C and C-D ring pairs have trans, cis, cis and trans junctions, respectively. The absolute configuration of the matrine molecule is 1S, 5S, 6S, 7S, 11R, 16R. Original Russian Text Copyright ? 2009 by Z. M. Jin, L. L. Ma, W. X. Wei, and Y. Q. Li The text was submitted by the authors in English. Zhurnal Strukturnoi Khimii, Vol. 50, No. 1, pp. 197–200, January–February, 2009.     

广西全州县金槐生产存在的问题及发展对策

针对广西全州县金槐生产的现状和存在问题,提出培育加工型龙头企业,进一步优化生产布局,完善标准化体系,打造名优品牌,提高市场竞争力,促进金槐产业升级等方面的发展建议和对策。     

Determination of quinolizidine alkaloids in Sophora flavescens and its preparation using capillary electrophoresis

A simple and accurate capillary electrophoresis method was developed for the determination of four quinolizidine alkaloids in Sophora flavescens and Kuhuang injection. Optimum separation of the analytes was obtained on a 65 cm x 75 microm i.d. uncoated fused-silica capillary using a aqueous buffer system of 60 mmol L(-1) sodium borate at pH 8.5, with applied voltage and capillary temperature of 12 kV and 25 degrees C, respectively. Detection wavelength was set at 204 nm and jatrorrhizine was used as the internal standard. Good linear relationships between peak-area ratios and concentrations of the analytes were observed over the concentration range 0.044-0.792 mg mL(-1) for matrine, 0.142-1.926 mg mL(-1) for oxymatrine, 0.0377-0.3393 mg mL(-1) for sophocarpine and 0.0664-1.062 mg mL(-1) for sophoridine. The recoveries of four alkaloids ranged between 93.08 and 101.4% with relative standard deviations from 0.7 to 9.2% (n = 6) as determined by standard addition. The limits of detection for four alkaloids were determined to be over the range 8.8-48.0 microg mL(-1). Contents of four alkaloids in Sophora flavescens and three alkaloids in Kuhuang injection were successfully determined under the optimum conditions.     

毛细管区带电泳法研究不同炮制方法对槐花、槐米中芦丁和槲皮素含量的影响

建立了一种毛细管区带电泳(CZE)法测定芦丁和槲皮素的方法,利用该方法研究了不同炮制方法对槐花、槐米中芦丁和槲皮素含量的影响。研究了缓冲溶液pH和浓度、分离电压、有机添加剂的浓度和进样时间对分离的影响,在优化条件下,上述两种物质在5 min内实现良好分离。芦丁和槲皮素峰面积和质量浓度分别在0.04~1.8和0.02~2.0 g.L-1浓度范围内呈线性关系,线性相关系数分别为0.999 2和0.999 4,检出限分别为8和5 mg.L-1。试验结果表明,炮制方法影响槐花和槐米中芦丁、槲皮素的含量。     

Simultaneous determination of phytoestrogens in different medicinal parts of Sophora japonica L. by capillary electrophoresis with electrochemical detection

A high-performance capillary electrophoresis with electrochemical detection (CE-ED) method has been developed for the determination of phytoestrogens from the pericarps and seeds of Sophora japonica L. in this work. Genistin, genistein, rutin, kaempferol and quercetin are important bioactive constituents in these plants. The effects of several factors such as the acidity and concentration of running buffer, the separation voltage, the applied potential and the injection time on the CE-ED procedure were investigated. Under the optimum conditions, the five analytes could be well separated within 18 min in a 75 cm length capillary (i.d. 25 microm) at the separation voltage of 16 kV in a 50 mmol L(-1) borax running buffer (pH 9.0). A 300 microm diameter carbon disk electrode was used as the working electrode positioned carefully opposite the outlet of the capillary in a wall-jet configuration at the potential of +950 mV (vs SCE). Detection limits (S/N = 3) ranged from 1.1 x 10(-7) to 2.8 x 10(-7) g mL(-1) for all fi ve analytes. This method was successfully used to analyse dried Flos sophorae immaturus, pericarps and seeds of dried Fructus sophorae after a relatively simple extraction procedure, and the assay results were satisfactory.     

苦参种子抑菌活性研究

为考察苦参（Sophora flavescens）种子的抑菌活性,采用超声法对其成分进行提取.首先比较苦参种子与苦参根的抑菌作用,然后再分别采用不同极性溶剂（石油醚、三氯甲烷、乙酸乙酯和乙醇）提取苦参种子中的成分,考察苦参种子中抑菌成分存在部位.结果表明,苦参种子的乙醇提取液对大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌的抑菌效果均较明显,且优于苦参根的乙醇提取液;苦参种子的乙醇提取液和三氯甲烷提取液都有抑菌作用,其中乙醇提取液的抑菌效果更好.由此可以得出在抑菌作用上,可以用苦参种子的醇提取液代替苦参根.     

苦豆子茎秆中生物碱类化学成分研究

为研究新疆苦豆子茎秆中生物碱类化学成分。采用酸水提取法提取苦豆子茎秆中的生物碱,采用多种柱层析、重结晶方法分离纯化,运用化合物的理化性质和光谱数据进行化合物的结构鉴定。结果显示,从苦豆子茎秆中共分离得到5个生物碱,分别鉴定为槐啶碱(1)、槐果碱(2)、槐胺碱(3)、黄华碱(4)和左旋-O-乙酰基膺靛叶碱(5)。本研究中的化合物5为首次从本植物中分离得到。