排序方式: 共有105条查询结果,搜索用时 15 毫秒
41.
Following a detailed study, a reversed-phase high performance liquid chromatographic method (HPLC) has been developed and validated for analysis of three bioactive alkaloids, matrine, sophoridine and oxymatrine, in Sophora flavescens Ait. HPLC separation of the alkaloids was performed on a Kromasil C(18) column and detected by ultraviolet absorbance at 208 nm. The column temperature was maintained at 40 degrees C. A mobile phase composed of 0.01 mol/L KH(2)PO(4) buffer-methanol-triethylamine in the ratios 94:6:0.01 (v/v) was found to be the most suitable for this separation at a fl ow-rate of 1.0 mL/min and enabled the baseline separation of the three analytes free from interferences with isocratic elution. The analysis time was 24 min per injection. The calibration was linear in the range of 0.2-120.0 micro g/mL for matrine, 0.2-115.2 micro g/mL for sophoridine and 0.2-110.4 micro g/mL for oxymatrine, respectively. For assaying Sophora Flavescens Ait. samples, the relative standard deviations were 2.0% for matrine, 2.8% for sophoridine and 1.8% for oxymatrine analysis. The average recoveries of matrine, sophoridine and oxymatrine were 93.9, 95.3 and 93.5% for the Sophora flavescens Ait. samples, respectively. The method has been successfully applied to the simultaneous determination of matrine, sophoridine and oxymatrine in Sophora Flavescens Ait. samples collected in different habitats. 相似文献
42.
对苦参(Sophra flavescens Ait)。进行了核型分析,结果表明:染色体数目为2n=18,核型公式为K(2)=18=10m+8sm,核型属“2A”型。核型不对称系数为59.42%,染色体的相对长度组成为2n=18=6M2+12M1。 相似文献
43.
IAA,IBA,NAA和2,4—D对槐树试管苗生根的影响 总被引:7,自引:0,他引:7
IAA和IBA诱导槐树试管苗形成的不定根细而长,直接来源于茎,根系发达.NAA诱导试管苗基部先形成愈伤组织,然后由愈伤组织上产生不定根,其根短而粗.NAA诱导的生根率最高,但其根生长缓慢,表面常愈伤组织化.2,4-D促进苗基部产生大量的愈伤组织,对生根有抑制作用 相似文献
44.
山东6种植物的染色体研究 总被引:6,自引:0,他引:6
杨德奎 《山东师范大学学报(自然科学版)》2001,16(3):305-312
对山东6种植物进行了染色体研究.白英(Solanum lyratum Thunb.)染色体数目为2n=24。核型公式K(2n)=2x=24=20m 4sm,核型“1A”;葎草(Humulus scandens(Lour.)Merr.)染色体数目为2n=16,核型公式K(2n)=2x=16=8m 8sm,核型“2A”;白刺花(Sophora vicifoliaHance.)染色体数目为2n=18。核型公式K(2n)=2x=18=2M 14m 2sm,核型“1A”;有斑百合(Lilium concolor var .pulchellum(Fisch.)Regd.)染色体数目为2n=24,核型公式K(2n)=2x=24=4m 2sm 6st 12t,核型“3A”;野韭(Allium ramosum L.)染色体数目为2n=16 1B,核型公式K(2n)=2x=16=12m 2sm 2st 1B,核型“2A”;狼尾花(Lysimachia barystachys Bge.)染色体数目为2n=24,核型公式K(2n)=2x=24=14m 10sm,核型“2A”。 相似文献
45.
《Journal of separation science》2018,41(7):1691-1703
Double‐templated molecularly imprinted polymers with specific recognition of three matrine‐type alkaloids were prepared using matrine and oxymatrine as the template molecules. An approach based on double‐templated molecularly imprinted solid‐phase extraction coupled with high‐performance liquid chromatography and tandem mass spectrometry was then developed to extract and purify matrine, oxymatrine, and sophocarpine from Sophora moorcroftiana in the Tibetan plateau herbs. The polymers were characterized by Fourier‐transform infrared spectroscopy and scanning electron microscopy. Their adsorption characteristics were evaluated using adsorption kinetics, isotherms, selectivity, and recycling experiments. This polymer exhibited excellent molecular recognition ability and good selectivity. The obtained polymers as adsorbent was further used for the determination of three matrine‐type alkaloids coupled to high‐performance liquid chromatography with tandem mass spectrometry, the recoveries of three matrines spiked at three concentration levels in samples were 73.25–98.42% (n = 5) with a relative standard deviation less than 6.82%. The limits of detection for the method were 9.23–15.42 μg/kg (S/N = 3). This proposed method was assessed to be an effective method for simultaneous extraction, isolation, and identification of matrine, oxymatrine, and sophocarpine from Sophora moorcroftiana. 相似文献
46.
This study analysed the use of sophora flower bud extract for dyeing and the resulting colour character and fastness of dyed silk fabric. The pigment composition on the silk fabric and recycling of this extract were also studied. The results indicated that the dyed silk fabric possessed good washing, rubbing and perspiration fastness, and the pigment composition on the silk fabric was mainly rutin and quercetin. The average recovery rate of the dye was 55.00%. These results demonstrate that the sophora flower bud extract is an effective natural dye. 相似文献
47.
48.
49.
Chi Zhang Yan‐Min Wang Feng‐Chun Zhao Liang‐Mian Chen Qi‐Wei Zhang Hui‐Min Gao Zhi‐Min Wang 《Helvetica chimica acta》2014,97(11):1516-1525
Two new compounds, (?)‐(6aR,11aR)‐4‐methoxy‐8,9‐(methylenedioxy)pterocarpan 3‐O‐β‐D ‐glucopyranoside ( 1 ) and 5‐hydroxy‐7‐methoxyisoflavone 4′‐O‐β‐D ‐xylopyranosyl‐(1→6)‐β‐D ‐glucopyranoside ( 2 ), were isolated, together with 30 known compounds from the stems and leaves of Sophora flavescens Aition . Their structures were elucidated by extensive spectroscopic analysis, including HR‐ESI‐MS data. A preliminary comparison of phenolic metabolite profiles, based on the qualitative HPLC analysis, indicated that the composition of the roots and the aerial parts were significantly different. 相似文献
50.
利用苦豆子的子叶进行愈伤组织的诱导与继代,筛选出诱导愈伤组织及继代时的最佳培养基.实验发现各种培养基均能诱导出愈伤组织,0.5—1.0mg/L的2,4-D和0.5—2.0mg/L的细胞分裂素6-BA或与Zt共同添加,诱导的愈伤组织状态较好,且当NAA(浓度为0.5mg/L)存在时可改善愈伤组织的状态;浓度为2.0mg/L的Kt和1.0mg/L以下的2,4-D、NAA分别或同时使用时同样可得状态较好的愈伤组织.低浓度的2,4-D、6-BA混合使用或添加1.0mg/L以下的NAA是愈伤组织继代时较好的激素组合,同时观察到0.5mg/L2,4-D和0.5mg/L Kt以及0.5mg/L2,4-D、0.2m-g/LNAA和0.2mg/LTDZ两种组合也能较好的继代愈伤组织.愈伤组织的去褐化除调节激素的浓度与配比外,还采取了其他的去褐化措施,发现添加0.1%活性炭的条件下快速继代(2d继代一次)以及调整基本培养基是较好的去褐化措施. 相似文献