长爪沙鼠日本血吸虫病动物模型的建立

探讨了用长爪沙鼠建立日本血吸虫病动物模型.实验方法为采用腹部贴片法感染血吸虫尾蚴,每只沙鼠感染（120±2）条尾蚴,于5周后解剖,做病理观察.结果表明：肝脏表面出现粟白色虫卵结节,肝脏和肠壁HE染色后观察到虫卵肉芽肿产生.因此认为采用长爪沙鼠可成功建立日本血吸虫病感染模型,为实验研究血吸虫防治奠定基础.     

封洲禁牧、净化草洲、控制湖区血吸虫病的研究--南昌县泾口乡草洲试点工作小结

介绍了南昌县泾口乡５４４ ．５ ｈｍ２ 草洲实施封洲禁牧２ 年（１９９６ ～１９９７） 后的结果，草洲的阳性钉螺由０ ．００２ １９ 只／０ ．１１ ｍ２ 下降到零；耕牛血吸虫病由４ ．０１ ％ 下降至０ ．４６ ％ ；居民血吸虫病由７ ．４４ ％ 下降至１ ．８１ ％ ，达到有螺无害控制草洲血吸虫病流行的目的。     

2004年～2008年南昌高新开发区血吸虫病疫情调查分析

对2004年-2008年南昌高新开发区血吸虫病疫情进行调查分析,探讨今后防控对策。通过现场考察、座谈、访问、查看资料,统计分析各项防控指标。5年来该区未发生一例急感,人群发病率降至0.12%,救治了几乎所有能挽救的晚血病人,行政村的疫情层次已明显地向后推移,近2年一、二类疫情村已被取消,耕牛阳性率已降到0.73%,灭螺面积达2 267.79 hm2,压缩有螺面积276 hm2,消灭感染螺面积77 hm2。通过以控制传染源为主的综合措施,全区血防成效显著,大湖区封洲禁牧应属一个整体,要加强实行联防联控才能加速成效。     

动物血吸虫病农业综合防治试验研究

为了探索控制血吸虫病疫情的有效对策,2004年到2008年选择在川兴、大兴、磨盘三个乡镇开展了"四个突破"试验,取得了显著的灭螺防病效果与经济效益;钉螺面积下降了53.11%,人感染率下降0.23%,病畜阳性率下降了0.41%,在22个乡镇51个村推广应用,增收节支3969.65万元。     

血吸虫病防控技术集成研究与示范的效果评价

为评价和分析血吸虫病防控技术集成研究与示范的疫情效果。通过在示范区实施控制血吸虫病流行的综合措施,并开展血吸虫病疫情调查及相关问卷调查。结果表明,通过连续3年的血吸虫病防控技术的实施,居民血吸虫感染率由3.57%降至0.83%;耕牛血吸虫感染率由17.14%降至0;示范区洲滩未发现阳性钉螺。建立以控制传染源为核心的血吸虫病综合防控技术新体系。     

Ultrastructural characterization of cells in primary cultures from different adult tissues of Biomphalaria tenagophila TAIM,a strain that is absolutely resistant to Schistosoma mansoni infection

The etiological agent of schistosomiasis in Brazil, Schistosoma mansoni, requires an obligatory passage through Biomphalaria snails to complete its life cycle. In these intermediate hosts, interaction with the parasite is mediated by humoral factors and hemocytes by mechanisms that are not yet fully understood. Extant studies exploring these processes are usually conducted through experimental infection of Biomphalaria with S. mansoni miracidia. Thus, tissue-derived cultures of Biomphalaria may be useful in increasing the understanding of that interaction at cellular level. However, in the absence of morphological characterization of those cells in culture, the application of such models is delayed. In the present work, we cultured different tissues of B. tenagophila, the second most important host of S. mansoni in Brazil, using a strain that is naturally and absolutely resistant to S. mansoni infection. This decision was driven by the view that this strain might be provided with the most effective response against parasite infection. Primary cultures were successfully established from nine Biomphalaria tissues and the respective cells in culture were ultra structurally described. Attention was particularly devoted to cells derived from mantle cavity and kidney tissues. Although they have been considered important centers for hemocyte production in Biomphalaria, no detailed cell characterization is available in the pertinent literature. Herein, kidney-derived cells partially shared hematoblast characteristics. Moreover, under optical microscopy, kidney cells in culture were very similar to those derived from amebocyte-producing organ (APO) cultures, which have been recently shown to be capable of eliminating S. mansoni sporocysts in vitro. Based on the close resemblance of those cultures and their anatomical proximity inside the mantle cavity, we suggest the effective participation of Biomphalaria kidney cells in hematopoiesis and in host response to S. mansoni infection.