Activation of trisacryl gels with chloroformates and their use for affinity chromatography and protein immobilization

In this study we describe the activation with chloroformates of Trisacryl-GF-2000, a new synthetic gel support that is stable, hydrophilic, and contains large amounts of hydroxyl groups available for activation. Of all the reagents tested, the activation withN-hydroxysuccinimide-chloroformate andp-nitrophenylchloroformate in organic solvents provides the best activation yield and subsequent coupling. When Trisacryl was activated in acetone with the chloroformates in the presence of 4-dimethylaminopyridine as base and catalyst, up to 30% of the hydroxyl groups, (i.e., 1/repeating unit) could be activated. Amino-containing ligands and proteins could be coupled to these carriers at pH 8 or higher. For better results in affinitychromatographic applications, spacers of ε-amino caproic acid or diaminohexane were introduced. The efficacy of these columns was demonstrated by purification of enzymes, antibodies, and antigens. The performance of these new columns were compared with that of Sepharose columns activated in various ways. In every case, the properties of the Trisacryl support proved superior with particular reference to the purity of the product obtained.     

The vibrational analysis of methylarsonic acid,trideuteromethylarsonic acid and their anions

Raman and IR spectra of methylarsonic acid, CH₃AsO₃H₂, trideuteromethylarsonic acid, CD₃AsO₃H₂ and their anions in aqueous solution and the solid state are discussed. Some results of a general valence force field and a Urey-Bradley force field are reported.     

Using a process-centered approach to minimize the effort of compliance

 The complexity of different quality standards can, in principle, be covered by different approaches and strategies. In-depth process mapping of quality control (QC) work streams was used by the analytical laboratories of Lonza AG to show up the principle differences in being compliant to different quality systems. The results identified two main drivers for all necessary actions: process-related activities and infrastructure-related activities. In addition, a clear indication of the economic impact of these driving forces was gained, which led the laboratories to decide on a process-oriented approach. This approach has the advantage of being able to reflect the different demands of different quality assurance (QA) regulations within the same QC organizational structure. Following the process helps avoid unnecessary efforts in analytical work and represents a very economical approach, at the same time, providing high flexibility to react to different QA or customer demands. Received: 5 July 2002 Accepted: 12 November 2002 Acknowledgements The process-oriented approach resulted from many, very challenging discussions for which I would like to thank the staff of my organization (Analytics & QC), especially, the QA staff and the LIMS team. Presented at Analytica Conference, 23–26 April 2002, Munich, Germany Correspondence to B. Ciommer     

一种基于DSP的JPEG2000视频压缩系统的实现

JPEG2000是国际标准化组织ISO和国际电信联盟ITU T共同制定的新一代图像压缩标准.它是基于EB COT算法,使用DWT,采用两层编码策略, 能够获得较好的压缩率.介绍了JPEG2000的基本特点,概述了其图像编码系统.详细描述基于DSP的JPEG2000硬件压缩系统各个硬件的选择以及具体的实现过程.     

基于代理服务器网络系统的设计与实现

IP地址资源已日益紧张,早已不能满足每 1台终端占用 1个IP的需要,阐述了利用网络代理服务器,合理地使用计算机局域网络技术,组建一个经济、可靠、稳定的网络系统。     

SQL注入漏洞的分析及防范

阐述了SQL注入的原理,对SQL注入的一般思路进行了详细分析;通过整形参数和字符串参数来判断是否存在注入漏洞,再通过判断数据库类型和数据库表名及字段名来达到注入的不同目的;介绍了多种注入方式,并从服务器管理员和程序员2个方面对SQL注入漏洞提出了防范措施。     

浅析PowerBuilder下动态SQL语句

在开发应用系统时,常常用到SQL语句提高程序效率,尤其是动态SQL语句,使程序代码简练、灵活、通用,不仅降低了编程的工作量,而且也降低以后对代码的维护量,能够取得事半功倍的效果.本文介绍在PowerBuilder下的几种动态SQL语句的作用和用法     

cdma2000 1x EV-DO前向链路高速分组数据传输中的调度算法

在保证用户间公平性的前提下,为尽量提高cdma2000的增强型1xEV DO系统前向链路的数据吞吐量,通过讨论现有的多种调度算法原理,分析了影响系统吞吐量和公平性的因素,在此基础上提出将用户平均请求传输速率、限制用户最小平均传输速率和系统最小吞吐量的影响同时引入调度算法的思想,通过在CadenceSPW软件平台上的仿真研究表明,改进后的调度算法在保持公平性的前提下,有效提高了系统前向链路吞吐量.     

数据库中图像存储的策略与方法研究

主要阐述BLOB数据类型,并利用该类型来完成图像数据在数据库中的存储和查询操作,从而解决了传统的图像数据存储在数据库服务器的文件目录下的管理模式。     

平板显示器件的多种灰度显示方案

介绍了各种灰度实现的方案，分析其灰度级实现原理和各自的特点，并着重描述子场和PWM方法；同时介绍一款专用灰度调制芯片BHL2000及其在动态图像显示中的实际应用方案，该芯片已在目前国内最大的FED驱动电路中采用。较其它FPD灰度调制方面的文章，本文的分析更为全面，并举出应用实例。