动物血液学的比较研究 Ⅱ．几种脊椎动物的血象、血清蛋白和血液流变学的观察

本文报导了虎纹蛙、黑眶蟾蜍的血象、血清蛋白，中国雨蛙的白细胞分类计数和罗非鱼、家鸽、家兔的血液流变学的研究结果，为比较生理学研究提供基础资料．     

黄连抗菌作用的热动力学研究

使用热活性检测仪测定了白色葡萄球菌、福氏１ａ，福氏１ｂ和福氏５ｂ四种细菌在３７℃正常条件及不同黄连浓度下的生长代谢热谱曲线。根据细菌生长的Ｍａｌｒｈｕｓ模型，计算出了各细菌在相应条件下生长速率常数及传代时间，并拟合出了黄连水剪液抑制细菌生长的临界剂量，提出了一种新的检测天然中药抗菌作用的方法。     

中国红豆杉悬浮细胞培养动力学的研究

考察了中国红豆杉悬浮细胞培养中细胞生长的动态变化，培养基中主要营养成分的消耗，细胞的氧消耗速率，以及次级代谢产物紫杉醇的生产状况。实验结果表明，细胞比生长速率为０．１２９／ｄ，紫杉醇的积累与细胞生长呈部分相关在，培养静止期达到高峰。     

金莲花注射剂精制新工艺研究

研究精制金莲花注射剂的最佳工艺条件.水提醇沉法和大孔吸附树脂吸附法联合应用对金莲花粗提物进行精制,紫外分光光度法测定混合物中总黄酮的含量,考察最佳精制工艺条件.该方法精制金莲花总黄酮的最佳工艺条件为金莲花提取物上样质量浓度40 mg/mL(按总黄酮计),总黄酮最大吸附量为32.5 mg/mL,洗脱流速为1.5 BV/h,洗脱剂为30%乙醇,所得的总黄酮纯度达到81.7%.该方法适合对金莲花注射剂的精制.     

衢州中国石龙子形态特征和雌体繁殖输出

通过报道浙江衢州中国石龙子成体形态特征的两性异形和雌体繁殖输出，测定了雌雄两性个体的体长、体重、头长和头宽、附肢长和腹长，除腹长无显著两性差异外，其他形态特征均表现为雄性个体大于雌性个体。衢州种群雌体年产单窝卵，平均窝卵数，窝卵重和平均卵重分别为16．8，8．767g和0．589g．     

太白红杉种群生殖对策研究Ⅰ生育力和种子重量

研究了太白红杉种群生育力和种子重量与年龄及海拔的关系．结果表明：(1)南坡的太白红杉成熟年龄比北坡早．(2)太白红杉种群的生育力随着年龄的增大而增大，但到一定年龄后又呈下降趋势．南坡太白红杉在70龄左右时生育力开始降低，北坡太白红杉在90龄左右生育力下降；北坡太白红杉种群的生育力随海拔的升高逐渐增大，大约到海拔3300m左右后逐渐降低；南坡太白红杉种群的生育力则一直随海拔升高而逐渐增大．(3)太白红杉种子的重量(千粒重)随着个体年龄的增大逐渐增加，但南坡在60龄左右时又逐渐变轻；种子重量随海拔的升高表现为先增加，后下降的趋势，但在北坡种子重量开始下降的海拔要比南坡低．     

Tupisteroide A–C,three new polyhydroxylated steroidal constituents from the roots of Tupistra chinensis

Three new steroidal compounds with polyhydroxy groups, tupisteroide A–C (1–3), were obtained from the roots of Tupistra chinensis, together with one known compound (4) that was isolated from this plant for the first time. The structures of tupisteroide A–C were determined on the basis of one‐ and two‐dimensional NMR spectroscopy, including ¹H–¹H Correlation Spectroscopy, Heteronuclear Multiple Bond Correlation, and Heteronuclear Single Quantum Coherence experiments. The isolated compounds were evaluated for their cytotoxic activities against A549, HepG2, and CaSki cancer cell lines in vitro. Among them, compounds 1, 2, and 4 did not show significant inhibitory activity, but compound 3 showed cytotoxicity against A549 cancer cell lines with IC₅₀ values of 25.0 μM. Copyright © 2012 John Wiley & Sons, Ltd.     

Ion pair assisted micro matrix solid phase dispersion extraction of alkaloids from medical plant

A novel micro matrix solid phase dispersion method was successfully used for the extraction of quaternary alkaloids in Phellodendri chinensis cortex. The elution of target compounds was accomplished with sodium hexanesulfonate as the eluent solvent. A neutral ion pair was formed between ion-pairing reagent and positively charged alkaloids in this process, which was beneficial for selectively extraction of polar alkaloids. Several parameters were optimized and the optimal conditions were listed as follows: silica gel as the sorbent, silica to sample mass ratio of 1:1, the grinding time of 1 min. The exhaustive elution of targets was achieved by 200 µL methanol/water (9:1) containing 150 mM sodium hexane sulfonate at pH 4.5. The method validation covered linearity, recovery, precision of intraday and interday, limits of detection, limits of quantitation, and repeatability. This established method was rapid, simple, environmentally friendly, and highly sensitive.     

UFLC–MS/MS method for simultaneous determination of six lignans of Schisandra chinensis (Turcz.) Baill. in normal and insomniac rats brain microdialysates and homogenate samples: towards an in‐depth study for its sedative‐hypnotic activity

Schisandra chinensis (Turcz.) Baill., a traditional Chinese medicine, has been clinically used for the treatment of insomnia for centuries. The insomnia mechanism and the possible active ingredients of S. chinensis remain largely unknown. The objective of this study was to develop a method to detect its components which could pass through the blood brain barrier (BBB) by determining the brain microdialysate and brain tissue homogenate samples and then obtain the pharmacokinetic profile in brain for comprehensive understanding of its hypnotic clinical efficacy. Therefore, an efficient, sensitive and selective ultra fast liquid chromatography/tandem mass spectrometry method for the simultaneous determination of six sedative and hypnotic lignans (schisandrin, schisandrol B, schisantherin A, deoxyshisandrin, γ‐schisandrin and gomisin N) of Schisandra chinensis (Turcz.) Baill. in rat brain tissue homogenate and brain microdialysates has been developed and validated. The analysis was performed on a Shim‐pack XR‐ODS column (75 mm × 3.0 mm, 2.2 µm) using gradient elution with the mobile phase consisting of acetonitrile and 0.1% formic acid water. The method was validated in brain homogenate and microdialysate samples, which all showed good linearity over a wide concentration range (r² > 0.99), and the obtained lower limit of quantification was 0.1 ng · ml^?1 for the analytes in brain microdialysate samples. The intra‐ and inter‐day assay variability was less than 15% for all analytes. The study proved the six lignans, as sedative and hypnotic ingredients, could pass through the BBB with brain targeting, distributed mainly in the hypothalamus and possessed complete pharmacokinetics process in brain. The results also indicated that significant difference in pharmacokinetic parameters of the analytes was observed between two groups, while absorptions of these analytes in insomniac group were significantly better than those in normal group. Copyright © 2013 John Wiley & Sons, Ltd.     

An efficient strategy for the extraction and purification of lignans from Schisandra chinensis by a combination of supercritical fluid extraction and high‐speed counter‐current chromatography

An efficient strategy for extracting and separating five lignans from Schisandra chinensis (Turcz.) Baill has been developed using supercritical fluid extraction (SFE) and high‐speed counter‐current chromatography (HSCCC) in the present study. First, the extraction was performed by a preparative SFE system under 15 MPa of pressure at 36°C for 4 h. Then, the SFE extract was successfully separated and purified by HSCCC with a two‐phase solvent system composed of n‐hexane/ethyl acetate/methanol/water (6:4:5:5, 6:4:6:4, 6:4:8:2, v/v) in a stepwise elution mode. The fractions were analyzed by HPLC, and the chemical structures of the products were identified by ESI‐MS and ¹H NMR spectroscopy. As a result, a total of 12.5 mg of schisandrin at 98.0% purity, 7.1 mg of gomisin A at 98.1% purity, 1.8 mg of schisantherin B at 93.3% purity, 4.4 mg of deoxyschisandrin at 92.9% purity, and 6.8 mg of γ‐schisandrin at 89.1% purity were obtained from 300 mg crude extract in a one‐step purification.