On-channel base stacking in microchip capillary gel electrophoresis for high-sensitivity DNA fragment analysis

We evaluated a novel strategy for high-sensitivity DNA fragment analysis in a conventional glass double-T microfluidic chip. The microchip allows for a DNA on-channel concentration based on base stacking (BS) with a microchip capillary gel electrophoretic (MCGE) separation step in a poly(vinylpyrrolidone) (PVP) sieving matrix. Depending if low conductivity caused a neutralization reaction between the hydroxide ions and the run buffer component Tris+, the stacking of DNA fragments were processed in the microchip. Compared to a conventional MCGE separation with a normal electrokinetic injection, the peak heights of 50-2650-base pair (bp) DNA fragments on the MCGE-BS separation were increased 3.9-8.0-fold. When we applied the MCGE-BS method to the analysis of a clinical sample of bovine theileria after PCR reaction, the peak height intensity of the amplified 816-bp DNA fragment from the 18S rRNA of T. buffeli was enhanced 7.0-fold compared to that of the normal injection method.     

Sol-gel approach to in situ creation of high pH-resistant surface-bonded organic-inorganic hybrid zirconia coating for capillary microextraction (in-tube SPME)

A novel zirconia-based hybrid organic-inorganic sol-gel coating was developed for capillary microextraction (CME) (in-tube SPME). High degree of chemical inertness inherent in zirconia makes it very difficult to covalently bind a suitable organic ligand to its surface. In the present work, this problem was addressed from a sol-gel chemistry point of view. Principles of sol-gel chemistry were employed to chemically bind a hydroxy-terminated silicone polymer (polydimethyldiphenylsiloxane, PDMDPS) to a sol-gel zirconia network in the course of its evolution from a highly reactive alkoxide precursor undergoing controlled hydrolytic polycondensation reactions. A fused silica capillary was filled with a properly designed sol solution to allow for the sol-gel reactions to take place within the capillary for a predetermined period of time (typically 15-30 min). In the course of this process, a layer of the evolving hybrid organic-inorganic sol-gel polymer got chemically anchored to the silanol groups on the capillary inner walls via condensation reaction. At the end of this in-capillary residence time, the unbonded part of the sol solution was expelled from the capillary under helium pressure, leaving behind a chemically bonded sol-gel zirconia-PDMDPS coating on the inner walls. Polycyclic aromatic hydrocarbons, ketones, and aldehydes were efficiently extracted and preconcentrated from dilute aqueous samples using sol-gel zirconia-PDMDPS coated capillaries followed by thermal desorption and GC analysis of the extracted solutes. The newly developed sol-gel hybrid zirconia coatings demonstrated excellent pH stability, and retained the extraction characteristics intact even after continuous rinsing with a 0.1 M NaOH solution for 24 h. To our knowledge, this is the first report on the use of a sol-gel zirconia-based hybrid organic-inorganic coating as an extraction medium in solid phase microextraction (SPME).     

吸附染料J-聚集体的氯化银微晶光电子特性

应用微波吸收相相敏介电谱技术测量吸附染料J-聚集体的立方体氯化银微晶中光电子的衰减特性,从而研究染料对氯化银微中光电子的影响.研究表明:在紫外光照射吸附机染料J-聚集体的氯化银时,染料能够加快光电子的衰减;光电子的变化规律与吸附染料浓度有关;说明染料增加了氯化银微晶中的隙间银离子浓度.可见光照射吸附有机染料的氯化银时,染料能够将染料激发态的光电子转移到氯化银中;显著地增加了氯化银微晶中的光电子强度;染料起到了敏化氯化银的作用.     

基于W eb和开源技术的“生产管理系统”的开发与应用

主要介绍了当今流行的W eb技术、面向对象的Java编程语言及一系列的开放源代码技术,并就美国一家公司的“生产管理系统”从原有的C/S结构移植到基于W eb的B/S结构的改造工程,论述了整个系统的移植过程,及其成功经验,最后总结了我们在进行企业信息化建设过程中可借鉴之处.     

从硅谷的成功看产学研合作中大学与科技园的关系

大学科技园是产学研合作的重要载体，对加速高校科技成果转化为生产力，促进产、学、研相结合，增强大学自我发展能力，提高办学效益发挥了重要的作用。众所周知，美国的硅谷是大学科技园的成功典范。本文拟从斯坦福大学与硅谷的关系分析，对比我国大学产学研合作中的实际做法，得出一些在高新技术产业化进程中可供借鉴的方法。     

PIA法制备MMCs骨架工艺及进展

综述了有机前驱体浸渍烧蚀法(PIA)制备金属基复合材料(MMCs)用金属间化合物或陶瓷网络结构预制体的工艺过程及技术方法，分析了其制备工艺特点，指出。PIA法是目前制备MMCs最方便有效的方法。并论述了工艺进展的情况，展望了其发展前景，提出下一步需要解决的问题。     

教育技术工作的管理原则

教育技术是对学习过程和学习资源进行的设计、开发、利用、管理和评价，其重要作用已成为我国教育技术工作者和教育行政管理部门的共识。在实践过程中，应从管理科学的层面上坚持系统性，突出重点，依靠制度管理的原则发挥其教学效益和社会效益，     

DataSocket技术在分布式故障录波系统中的应用

用通常的方法实现测控数据的网络传输，编程复杂，难度大，DataSocket技术可以很好地解决这个问题．对DataSocket技术进行了简要介绍．同时给出了用DataSocket技术实现分布式故障录波系统信息传输的具体方法，较好地解决了分布式故障录波系统的信息开放性问题．     

战略环境评价的理论及技术方法探讨

回顾了战略环境评价(SEA)的形成和发展过程，讨论了SEA实施的原则和影响因素，分析了SEA实施的战略分析框架、替代方案和影响减缓措施，并探讨了SEA的研究方法和技术．     

纳米科技的研究领域及前景展望

当物质小到纳米尺度时 ,就会出现新的现象 ,产生新的效应。纳米科技从三个方面进行研究 ,它的最终目的是以原子、分子为起点 ,制备具有特殊功能的产品 ,使这些产品的性能有明显的改善和提高。纳米科技的发展将引起一场几乎所有工业领域的革命。