Preparation, characterization and properties studies of quinine-imprinted polymer in the aqueous phase

The uniform-sized spherical molecularly imprinted polymers were successfully prepared through molecular imprinting technology by two-step seed swelling and mini-emulsion polymerization in the aqueous condition using quinine as template molecules and methacrylic acid (MAA) as functional monomer. The polymers were characterized by IR spectra, thermal-weight analysis, scanning electron microscope and laser particle size analysis. The properties of imprinted polymers were investigated in different organic phases and aqueous media. In the organic media, results suggested that polar interactions (hydrogen bonding, ionic interactions) between acidic monomer/polymer and template molecules are mainly responsible for the binding and recognition; whereas in the aqueous medium, a considerable recognition effect was also obtained where the ionic (electrostatic) interaction and hydrophobic interaction play an important role. The experiments of binding different substrates indicated that the MIPs possessed an excellent rebinding ability and inherent selectivity to quinine. __________ Translated from Zhongshan Dcocue Xuebao/Acta Scientianum Natralium University Sunyatseni, 2005, 44(3)(in Chinese)     

On-channel base stacking in microchip capillary gel electrophoresis for high-sensitivity DNA fragment analysis

We evaluated a novel strategy for high-sensitivity DNA fragment analysis in a conventional glass double-T microfluidic chip. The microchip allows for a DNA on-channel concentration based on base stacking (BS) with a microchip capillary gel electrophoretic (MCGE) separation step in a poly(vinylpyrrolidone) (PVP) sieving matrix. Depending if low conductivity caused a neutralization reaction between the hydroxide ions and the run buffer component Tris+, the stacking of DNA fragments were processed in the microchip. Compared to a conventional MCGE separation with a normal electrokinetic injection, the peak heights of 50-2650-base pair (bp) DNA fragments on the MCGE-BS separation were increased 3.9-8.0-fold. When we applied the MCGE-BS method to the analysis of a clinical sample of bovine theileria after PCR reaction, the peak height intensity of the amplified 816-bp DNA fragment from the 18S rRNA of T. buffeli was enhanced 7.0-fold compared to that of the normal injection method.     

Sol-gel approach to in situ creation of high pH-resistant surface-bonded organic-inorganic hybrid zirconia coating for capillary microextraction (in-tube SPME)

A novel zirconia-based hybrid organic-inorganic sol-gel coating was developed for capillary microextraction (CME) (in-tube SPME). High degree of chemical inertness inherent in zirconia makes it very difficult to covalently bind a suitable organic ligand to its surface. In the present work, this problem was addressed from a sol-gel chemistry point of view. Principles of sol-gel chemistry were employed to chemically bind a hydroxy-terminated silicone polymer (polydimethyldiphenylsiloxane, PDMDPS) to a sol-gel zirconia network in the course of its evolution from a highly reactive alkoxide precursor undergoing controlled hydrolytic polycondensation reactions. A fused silica capillary was filled with a properly designed sol solution to allow for the sol-gel reactions to take place within the capillary for a predetermined period of time (typically 15-30 min). In the course of this process, a layer of the evolving hybrid organic-inorganic sol-gel polymer got chemically anchored to the silanol groups on the capillary inner walls via condensation reaction. At the end of this in-capillary residence time, the unbonded part of the sol solution was expelled from the capillary under helium pressure, leaving behind a chemically bonded sol-gel zirconia-PDMDPS coating on the inner walls. Polycyclic aromatic hydrocarbons, ketones, and aldehydes were efficiently extracted and preconcentrated from dilute aqueous samples using sol-gel zirconia-PDMDPS coated capillaries followed by thermal desorption and GC analysis of the extracted solutes. The newly developed sol-gel hybrid zirconia coatings demonstrated excellent pH stability, and retained the extraction characteristics intact even after continuous rinsing with a 0.1 M NaOH solution for 24 h. To our knowledge, this is the first report on the use of a sol-gel zirconia-based hybrid organic-inorganic coating as an extraction medium in solid phase microextraction (SPME).     

从硅谷的成功看产学研合作中大学与科技园的关系

大学科技园是产学研合作的重要载体，对加速高校科技成果转化为生产力，促进产、学、研相结合，增强大学自我发展能力，提高办学效益发挥了重要的作用。众所周知，美国的硅谷是大学科技园的成功典范。本文拟从斯坦福大学与硅谷的关系分析，对比我国大学产学研合作中的实际做法，得出一些在高新技术产业化进程中可供借鉴的方法。     

PIA法制备MMCs骨架工艺及进展

综述了有机前驱体浸渍烧蚀法(PIA)制备金属基复合材料(MMCs)用金属间化合物或陶瓷网络结构预制体的工艺过程及技术方法，分析了其制备工艺特点，指出。PIA法是目前制备MMCs最方便有效的方法。并论述了工艺进展的情况，展望了其发展前景，提出下一步需要解决的问题。     

教育技术工作的管理原则

教育技术是对学习过程和学习资源进行的设计、开发、利用、管理和评价，其重要作用已成为我国教育技术工作者和教育行政管理部门的共识。在实践过程中，应从管理科学的层面上坚持系统性，突出重点，依靠制度管理的原则发挥其教学效益和社会效益，     

DataSocket技术在分布式故障录波系统中的应用

用通常的方法实现测控数据的网络传输，编程复杂，难度大，DataSocket技术可以很好地解决这个问题．对DataSocket技术进行了简要介绍．同时给出了用DataSocket技术实现分布式故障录波系统信息传输的具体方法，较好地解决了分布式故障录波系统的信息开放性问题．     

浅谈汽车维修专业技能型人才的培养

文章阐述了现代汽车技术的发展和维修市场对人才的需求，论述汽车维修专业应如何适应企业和市场的需求，培养出合格的技能型人才。     

山西在中部崛起战略中对科技的重大需求分析

文章针对山西经济社会发展的实际,分析了山西在中部崛起战略中对科技发展的重大需求。     

鄂尔多斯盆地北部天然气钻井技术研究

鄂尔多斯盆地北部(简称鄂北)工区钻井技术已配套成龙，优选的井身结构经济效益明显；3KP钾基聚合物钻井液、TG无固相完井液可保证井内安全；低密度水泥浆技术已成功应用。