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11.
Cytochrome P-450 isozymes represent a critical component of nature’s spectrum of detoxification catalysts that could be exploited
for bioremediation. The ethanol-inducible human cytochrome P-450 2E1 serves as a model eukaryotic P-450 that complements the
bacterial P-450 cam in dehalogenation and detoxification of environmental pollutants. We explored the construction of novel
chimeric P-450s using cytochrome P-450 camC and 2E1 genes. For construction of chimera 1 (478 amino acids, 55.14 kDa), 145 amino acids from the N-terminus of P-450 2E1 protein
(493 amino acids, 56.84 kDa) were replaced with 130 amino acids from the N-terminus of P-450 camC protein (415 amino acids,
46.66 kDa). In chimera 2 (525 amino acids, 60.24 kDa) the strategy involves replacement of 28 amino acids in the C-terminus
of chimera 1 with 75 amino acids from the C-terminus of P-450 camC gene. Homology models of both the chimeric proteins were
developed using SWISS-MODEL based on the known crystal structure of cytochrome P-450 camC, BM-3, 1DT6A, and 2C17A. The models
indicated that the proposed heme-binding site was intact, which is inevitable for catalytic activity of cytochrome P-450s.
The expression of chimera 1 and 2 genes in Escherichia coli DH5α was evident from light-pink cell pellets, protein band in sodium dodecyl sulfate polyacrylamide gel electrophoresis,
and diagnostic carbon monoxide-difference spectra. Our studies show that strategies can be developed to exploit the natural
diversity of the P-450 superfamily to generate chimeric biocatalysts that would provide new templates amenable to directed
evolution. 相似文献
12.
This paper studies the growth function, with respect to the generating set of edge identifications, of a surface group with fundamental domainD in the hyperbolic plane ann-gon whose angles alternate between /p and /q. The possibilities ofn,p andq for which a torsion-free surface group can have such a fundamental polygon are classified, and the growth functions are computed. Conditions are given for which the denominator of the growth function is a product of cyclotomic polynomials and a Salem polynomial.This work was supported in part by NSF Research Grants. 相似文献
13.
Sisto Baldo Giandomenico Orlandi 《Calculus of Variations and Partial Differential Equations》1996,4(4):369-384
We define a class
p
(M,N) of Sobolev maps from a manifoldM into a manifoldN, in such a way that each mapu
p
(M, N) has a well defined [p]-homotopy type, providedN satisfies a topological hypothesis. Using this, we prove the existence of minimizers in [p]-homotopy classes for some polyconvex variational problems. 相似文献
14.
V. Suresh 《K-Theory》1996,10(6):597-610
Let X be a smooth projective surface over a number field k. Let (CH0(X)) denote the Chow group of zero-cyles modulo rational equivalence on X. Let CH0(X) be the subgroup of CH
0(X) consisting of classes which vanish when going over to an arbitrary completion of k. Bloch put forward a conjecture asserting that this group is isomorphic to the Tate-Shafarevich group of a certain Galois module atttached to X. In this paper, we disprove this general conjecture. We produce a conic bundle X over an elliptic curve, for which the group (CH0(X) is not zero, but the Galois-theoretic Tate-Shafarevich group vanishes. 相似文献
15.
经过病原的分离鉴定、致病性试验及病仔猪防治效果的分析,确诊某集约化养猪场仔猪发生以“渗出性皮炎”为主的疾病为致病性白色葡萄球菌和大肠杆菌混合感染,通过采取综合的防制措施后,彻底控制本病的发生和流行。 相似文献
16.
苏胜昔 《河北大学学报(自然科学版)》1998,(4)
利用电子显微技术对几种金属材料拉伸断裂断口进行了分析,对它们的力学性能、拉伸磁性、断面微观结构进行了比较研究,得出了它们之间存在的一些特性,并从理论上作了一些初步的探讨。给出了铁磁体ΔE效应的应用。 相似文献
17.
Riddhi Shah 《Monatshefte für Mathematik》1993,115(3):191-213
For any locally compact groupG, we show that any locally tight homomorphism from a real directed semigroup intoM
1
(G) (semigroup of probability measures onG) has a shift which extends to a continuous one-parameter semigroup. IfG is ap-adic algebraic group then the above holds even iff is not locally tight. These results are applied to give sufficient conditions for embeddability of some translate of limits of sequences of the form {v
n
kn
} and M
1
(G) such that ()=
M
, for somek>1 and AutG (cf. Theorems 2.1, 2.4, 3.7). 相似文献
18.
B. Gyires 《Periodica Mathematica Hungarica》1978,9(1-2):109-113
This paper contains applications of theorems of [1] for quadratic statistics which have constant regression on linear statistics. Two theorems are proved. The first is a sufficient condition which assumes that the characteristic function of a sample is an entire function. The second gives a new characterization of the normal distribution. 相似文献
19.
Pohl D. W. Irniger V. Herrmann W. 《Applied Physics A: Materials Science & Processing》1978,17(4):361-365
A spectrophone with low resonant frequency compatible with the repetition frequency of a pulsed discharge CO laser is described.
The spectrophone is used for humidity detection in hydrogen and air. The simple and inexpensive system provides a detection
capacity of 10–100ppb/
. The responsivity of the resonant spectrophone is interpreted taking the dependence of the acoustical quality factor on the
geometry into account. 相似文献
20.
Shie-Shien Yang 《Annals of the Institute of Statistical Mathematics》1981,33(1):463-470
Summary Let (X
1,Y
1), (X
2,Y
2),…, (X
n,Y
n) be i.i.d. as (X, Y). TheY-variate paired with therth orderedX-variateX
rn is denoted byY
rn and terms the concomitant of therth order statistic. Statistics of the form
are considered. The asymptotic normality ofT
n is established. The asymptotic results are used to test univariate and bivariate normality, to test independence and linearity
ofX andY, and to estimate regression coefficient based on complete and censored samples. 相似文献