Chemical profile and antihyperlipidemic effect of Portulaca oleracea L. seeds in streptozotocin-induced diabetic rats

Hypolipidemic effect of Portulaca oleracea L. seed extract and its fractions have been studied on streptozotocin (STZ) at dose 75 mg/kg b.wt. After fractionation of the alcoholic extract; petroleum ether fraction was the most active fraction that decreased different hyperlipidemia biochemical parameters. After chromatographic analysis; oleamide, ethylpalmitate, β-amyrin, stigmasterol and β-sitosterol were identified. The GLC analysis of unsaponifiable matter revealed the presence of; lignoceric acid as a major constituent in the most bioactive fraction. In conclusion, petroleum ether fraction possessed a hypolipidemic effect in STZ-induced diabetic rats, which may be attributed to its phytosterols, fatty acid and amide compounds. The finding of the present investigation strongly demonstrates the potential of non-polar fraction of P. oleracea L. seed in combating hyperlipidemia in diabetic condition. So the petroleum ether fractions and its constituents can be used as hypolipdemic supplement in the developing countries towards the development of new therapeutic agents.     

Quantification of noradrenaline and dopamine in Portulaca oleracea L. by capillary electrophoresis with laser-induced fluorescence detection

In this paper, a rapid and sensitive method is described for the quantification of noradrenaline (NA) and dopamine (DA) in Portulaca oleracea L. After derivatization in a dark ultrasonic bath for 4 h, fluorescein isothiocyanate (FITC) derivatives of NA and DA were separated by capillary zone electrophoresis (CZE) in 5.5 min and detected with laser-induced fluorescence. In the concentration range (0.05-2.00 μM), the calibration curves reveal linear relationships between the peak-area for each analyte and its concentration (correlation coefficients: 0.9970 for NA and 0.9998 for DA). The recoveries are in the range of 94.6-97.3%. The detection limits for NA and DA are 1.02 and 0.34 nM, respectively.     

井冈山马齿苋多糖的体外抗氧化作用及总糖含量测定

目的探讨井冈山马齿苋多糖的体外抗氧化作用并测定其总糖含量。方法应用Fenton法、DPPH法和FRAP法比色法测定,共同评价井冈山马齿苋多糖的抗氧化作用。结果井冈山马齿苋多糖在体外能明显清除羟自由基、DPPH自由基和还原Fe~(3+),并且体外抗氧化作用均随其浓度的增大而增大;苯酚-硫酸比色法测定马齿苋多糖的总糖含量,总糖含量为23.56%。结论井冈山马齿苋多糖具有明显的体外抗氧化作用。     

A New Tricyclic Alkaloid from Portulaca oleracea L.

A new tricyclic alkaloid named portulacatone ( 1 ), i.e., 5,6‐dihydro‐8,9‐dihydroxy‐11H‐pyrrolo[2,1‐b] [3]benzazepin‐11‐one, together with eight known compounds, methyl 4‐hydroxyphenylacetate ( 2 ), p‐hydroxybenzaldehyde ( 3 ), vanillin ( 4 ), protocatechualdehyde ( 5 ), p‐hydroxybenzoic acid ( 6 ), iseluxine ( 7 ), oleracein E ( 8 ), and (+)‐(R)‐feruloyl malate ( 9 ) were isolated from aerial parts of Portulaca oleracea L. Their structures were elucidated based on spectroscopic analyses. Among them, compounds 1 – 7 and 9 were isolated from this medicinal plant for the first time. Compounds 1 and 7 showed dose‐dependent scavenging activities against DPPH (2,2‐diphenyl‐1‐picryl‐hydrazyl) free radical, with EC₅₀ values of 14.36 μM and 9.98 μM , respectively, more potent than the natural antioxidant vitamin C (EC₅₀ 20.72 μM ).     

马齿苋总黄酮含量的测定

本文对中草药马齿苋中总黄酮含量进行了提取、分离，用紫外分光光度计进行了测定。     

Antioxidant activity and fatty acids quantification in Sicilian purslane germplasm

Abstract

Portulaca oleracea is an annual succulent herb in the family Portulacaceae. It is a nutritious vegetable with high antioxidant properties and, it is among the richest plant source of ω-3 fatty acids, as well as a rich source of ω-6 fatty acids, ascorbic acid, tocopherols and beta-carotene. In the present study, three purslane populations under different Mediterranean environmental conditions for two years, for future valorization as novel food sources of omega-3 fatty acids, were evaluated. In particular, biomorphological characteristics, total phenols and fatty acids content were determined. The antioxidant activities were evaluated using 2,2-diphenyl-1-picrylhydrazyl assay. The population “Cas” appears to have higher antioxidant activity than the other two populations (“Cal” and “S. Ven”).The saturated fatty acid content is influenced only by the year of collection, while the polyunsaturated fatty acid by the populations. The most abundant unsatured fatty acids are linoleic and linolenic acids and “Cas” attained the highest contents.     

民族药太阳花的生药学鉴定

采用原植物、性状、显微、薄层鉴别方法对民族药太阳花进行系统的生药学鉴定,为其鉴别及应用提供科学依据.结果表明:通过原植物、性状鉴别、显微鉴别、薄层色谱鉴别能够很好的鉴定原植物.     

A new alkaloid from Portulaca oleracea L. and its antiacetylcholinesterase activity

A new alkaloid, (10E, 12E)-9-ureidooctadeca-10, 12-dienoic acid, named oleraurea (1) and 10 known compounds, p-hydroxybenzaldehyde (2), p-hydroxybenzoic acid (3), p-hydroxyacetophenone (4), benzamide (5), (E)-p-coumaramide (6), (E)-ferulamide (7), soyalkaloid A (8), β-carboline-3-carboxylic acid (9), 2, 3, 4, 9-tetrahydro-1H-pyrido [3, 4-b] indole-3-carboxylic acid (10), (1S, 3S)-1-methyl-1, 2, 3, 4-tetrahydro-β-carboline-3-carboxylic acid (11) were obtained from Portulaca oleracea L., in which, compounds 4, 5, 8–11 were isolated from the plant for the first time. The structure of the compound 1 was identified using spectroscopic methods including 1D and 2D NMR, HR-ESI-TOF-MS. The compounds 1, 5–11 presented anticholinesterase activities, but the P. oleracea extract (POE) presented very low anticholinesterase activity.     

马齿苋Portulaca oleracea的化学成分

从马齿苋（Portulaca oleracea）乙酸乙酯提取物中分离得到4个化合物,经MS和NMR等波谱技术分析,并结合文献对照,确定其结构分别为谷甾4-烯-3-醇(1),谷甾-5-烯-3-醇(2),friedelan-4α-methyl-3β-OH (3)和β-胡萝卜甙(4),其中化合物1,2,4为首次从该类植物中分...