Influence of location-dependent protuberance damage on cell viability

The influence of femtosecond laser-induced damages on viability of olfactory ensheathing cells (OECs) is investigated. Several cytokinetic processes including cellular damage, recovery and death are discussed. Using femtosecond laser with the power of 100 μW and cutting speed of 2 μm/s, we cut the cellular protuberance with smaller diameter twice in different locations, and then observe the viability of the damaged cells. Under the same conditions, the root of protuberance with larger diameter is cut six times to observe changes of cellular shape. Whether the damage is located in the end, middle or root of protuberance with smaller diameter, the cell viability can recover within 3 h. When the damage is located in the root of protuberance with larger diameter, the damaged cell will die in the way of oncosis. Cytokinetic phenomena including intracellular high Ca²⁺ concentration, cellular morphologic change, recovery and oncosis are discussed. Meanwhile, high Ca²⁺ concentration is observed after femtosecond laser surgery. Therefore, femtosecond laser surgery is an important tool for establishing cell damage model and studying cytokinetics. Supported by National High Technology Research & Development Program of China (Grant No. 2006AA04Z307), Author of National Excellent Doctoral Dissertation of PR China (Grant No. 2006039), National Natural Science Foundation of China (Grant No. 50775104), Natural Science Foundation of Jiangsu Province (Grant No. BK2006507) and Jiangsu Provincial Research Innovation Program for College Graduates (Grant No. CX07B_086z)     

简支梁损伤识别的特征方程曲线交点法

基于扭转弹簧模型的裂纹梁振动分析,推导了含单边边缘裂纹简支梁振动频率特征方程,提出了“特征方程曲线交点法”结合“附加质量”的方法．通过绘制相应于损伤梁前几阶固有频率的裂纹深度一位置的关系曲线,并求解其交点识别裂纹参数,解决了由于简支梁的对称性导致的损伤位置的误判问题．数值模拟结果显示,本文方法具有很好的识别精度．     

基于小波分析的弹性板损伤识别

以含有损伤的四边简支矩形板为研究对象,采用等效刚度代替板内损伤带的刚度,建立了含有多条损伤带的弹性板有限元模型.运用有限元法分析板的振动特性,由Lanczos法计算得到沿板边两个方向的转角模态.通过对板的转角模态进行db1连续小波变换,利用小波系数模极大值点判断板内损伤带的位置,给出了一种识别弹性板损伤的方法.通过对一含3条损伤带的四边简支矩形板进行损伤识别,验证了方法的有效性.     

基于应力损伤的电力机车传动系统损伤度模型

参照现有机械结构应力损伤基本原理,分析电力机车传动系统设备故障的本质原因,提出一种基于应力分析的电力机车传动系统损伤度模型.该模型采用迈因纳准则和可靠性原理,对电力机车传动系统设备进行大修期限估算,计算出各个关键设备的损伤度,从而估算出电力机车传动系统的大修期限.     

柳州市园林植物冻害调查及对策研究

在柳州市建城区,实地调查2008年1～2月柳州市园林植物冻害情况,分析冻害原因并提出相应的对策,为柳州园林植物的引种与保护提供参考依据.     

Evaluation of earthquake-induced structural damages by wavelet transform

The dynamic behavior of inelastic structures during an earthquake is a complicated non-stationary process that is affected by the random characteristics of seismic ground motions. The conventional Fourier analysis describes the feature of a dynamic process by decomposing the signal into infinitely long sine and cosine series, which loses all time-located information. However, both time and frequency localizations are necessary for the analysis of an evolutionary spectrum of non-stationary processes. In this paper, an analytical approach for seismic ground motions is developed by applying the wavelet transform, which focuses on the energy input to the structure. The procedure of identification of the instantaneous modal parameters based on the continuous wavelet transform (CWT) is given in detail. And then, a novel method using the auto-regressive moving average (ARMA), called "prediction extension”, is presented to remedy the edge effect during the numerical computation of the CWT. The effectiveness of the method is verified by the use of the benchmark model developed by the American Society of Civil Engineers (ASCE). Finally, a scale model with three-storey reinforced concrete frame-share wall structure is made and tested on a shaking table to investigate the relation between the dynamic properties of structures and energy accumulation and its change rates during the earthquake. The results have shown that the wavelet transform is able to provide a deep insight into the identity of transient signals through time-frequency maps of the time variant spectral decomposition.     

混凝土高频疲劳加速寿命试验

车载循环作用下的混凝土疲劳强度问题越来越受到人们的重视,而针对混凝土高频疲劳性能的研究不是很多,将高频疲劳试验方法引入到岩石类非金属材料的试验中,针对混凝土圆柱试件进行高频疲劳加速寿命试验研究.通过分析疲劳频率变化曲线的规律,解释混凝土高频疲劳的破坏机理,建立混凝土的轴向压缩非线性疲劳损伤累积模型.同时考虑到存活率,得出混凝土疲劳加速寿命失效概率的P-lgS-lgN双对数疲劳方程.     

钻井与完井对近井地层渗透性损伤的分析

为了研究钻井、完井过程中钻井液、完井液渗入近井地层以及固体颗粒沉淀造成的地层损伤对油井产量的影响,考虑钻井液、完井液对石油的驱替,将其渗入地层视为动界面的渗流力学问题进行研究.得到了钻井液、完井液的锋面位置与时间的关系式、损伤与未损伤区石油的压力分布、损伤与未损伤情形下油井产量的表达式.该成果对石油、天然气、煤层气开采具有一定的参考价值和指导意义.     

基于模糊随机损伤力学的模糊自适应有限元分析

为延拓非确定损伤理论研究以揭示损伤力学本质,基于损伤、概率、模糊隶属度在[0,1]区间上协调一致性提出模糊随机损伤力学观念.构造解释了3类损伤变量模糊性态及对应模糊映射分布,即降半分布、"秋千"分布和组合"秋千"分布,实现了随机损伤变量模糊化自适应生成与构建.依据扩张原理及随机损伤变量满足β概率分布,对模糊集上随机损伤变量的CDF、PDF积分修正,结合当量正态理论,将3类模糊随机损伤泛函引入本构方程,完成模糊随机有限元可靠度与模糊随机损伤同步分析.用自主研制分析软件对龙滩碾压混凝土坝做三维模糊随机损伤场力学性态研究,由复杂随机场与模糊随机损伤分析的成功融合证明分析方法是适用的、可行的、必要的.     

一类拟线性神经传播方程的紧LOD差分格式

通过引入变量将方程从形式上降阶,提出了求解一类拟线性神经传播方程的紧局部一维（LOD）差分格式,并应用能量方法给出了格式的误差估计,得到该格式在L^2模下具有O（Δt^2＋h^4）的精度．最后通过数值例子验证了算法的有效性．