笼养竹鼠的可行性实验

于 1995～ 1999年 ,进行了野生竹鼠的饲养 ,观察了竹鼠的生长与食物的种类、饲养条件有密切的关系 ,并作了详细记录。结果表明 ,野生竹鼠在人工饲养的条件下远较在野生环境下竹鼠的生长情况好     

北虫草及其活性成分的研究与开发

冬虫夏草为非常珍贵的天然药材,资源严重缺乏,价格已超过黄金,无法满足社会需求。因北虫草的化学成分和药理功能与冬虫夏草非常相似,其主要功能活性物质含量高于天然冬虫夏草,被作为冬虫夏草的最佳替代品,受到广泛关注和重视,本文总结了近年来北虫草及其生物活性成分的研究与开发结果。     

中华鳖红细胞发育过程的观察

通过骨髓、脾脏、肝脏、肾脏和外周血等器官涂片或印片观察来探讨中华鳖红细胞的产生器官、发育场所和发育各阶段的特征。结果表明骨髓是中华鳖红细胞的发源地，骨髓和脾脏是红细胞成熟的主要场所，在外周血和脾脏中亦可见少量未成熟或正在有丝分裂的红细胞，红细胞发育经过原始、幼稚和成熟等三个阶段，着重描述了红细胞发育各阶段的主要形态特征。     

A new method providing complementary explanation of the blood-enriching function and mechanism of unprocessed Angelica sinensis and its four kinds of processed products based on tissue-integrated metabolomics and confirmatory analysis

Angelica sinensis (AS) is a common Traditional Chinese Medicine used for tonifying blood in China. Unprocessed AS and its four kinds of processed products (ASs) are used to treat blood deficiency syndrome in the country. The different blood-tonifying mechanisms of ASs remain unclear. In this work, a novel method integrating metabolomics and hematological and biochemical parameters was established to provide a complementary explanation of blood supplementation mechanism of ASs. Our results revealed that different ASs exhibited various blood supplementation effect, and that AS parched with alcohol demonstrated the best blood supplementation effect. Eight metabolites from liver tissue and 12 metabolites from spleen tissue were considered to be potential biomarkers. These biomarkers were involved in four metabolic pathways. Correlation analysis results showed that l -aspartic acid and l -alanine (spleen tissue), linoleic acid, and l -cystathionine (liver tissue) exhibited a high positive or negative correlation with the aforesaid biochemical indicators. The blood-supplementation effect mechanism of ASs were related to four metabolic pathways. l -Aspartic acid and l -alanine (spleen tissue), linoleic acid, and l -cystathionine (liver tissue) were the four key metabolites associated with the blood supplementation effect of ASs. This study gives a complementary explanation of the blood supplementation effect and mechanism of action of ASs.     

重庆光皮木瓜鲜果挥发油成分的GC-MS分析

以重庆光皮木瓜鲜果为材料,采用水蒸气蒸馏法提取挥发油,并利用气相色谱-质谱(GC-MS)联用仪对其挥发油成分进行分析鉴定,结果从重庆光皮木瓜鲜果挥发油中分析和鉴定出101种化合物,占挥发油总量的88.43%,其中以酯类、烃类和醇类化合物为主,相对含量分别为65.8%、11.29%和7.22%.     

白木香[Aquilaria sinensis(Lour.)Gilg]的木质部结构及组织化学研究

利用离析技术、显微观察和组织化学鉴定等方法,对白木香的木质部结构及组织化学进行了研究。结果表明,木质部由5部分组成:髓心、内涵韧皮部、木射线、导管、纤维管胞。内涵韧皮部呈多孔型均匀分布;木射线单列(双列少见)异型Ⅲ;管孔排列为径向管孔团、复管孔、管孔链和单管孔;导管侧壁有互列纹孔式和附物纹孔;纤维管胞侧壁有单斜附物纹孔;径列条及栓塞首次在白木香中发现;进一步证实淀粉、还原性糖和挥发性成分在不同年龄的木质部里含量及储存部位存在差异。本研究为沉香的形成提供基本的解剖学证据。     

养殖模式及酸和硫化物胁迫对中华乌塘鳢血液生理生化指标的影响

【目的】比较红树林区3种不同生长环境下中华乌塘鳢(Bostrychus sinensis)的血液生理生化指标,并探究其对酸和硫化物胁迫的响应,为中华乌塘鳢红树林生态养殖提供参考。【方法】采集野生、红树林地埋管道生态养殖和池塘养殖的中华乌塘鳢成体,取血测定红细胞(RBC)含量、白细胞(WBC)含量及其组成、总蛋白(TP)含量、超氧化物歧化酶(SOD)活力、谷胱甘肽巯基转移酶(GST)活力和总抗氧化能力(T-AOC),并进行分析比较;采用不同强度的酸或硫化物胁迫中华乌塘鳢72h,每隔24h取血检测。【结果】在所测定的10项指标中,中华乌塘鳢红树林地埋管道生态养殖群体与池塘养殖群体间仅红细胞数量差异显著,而野生群体与养殖群体间有5项存在显著差异。红细胞数量差异表现为池塘养殖群体红树林地埋管道生态养殖群体野生群体。野生群体的中性粒细胞百分比显著高于养殖群体,淋巴细胞正好相反。水体pH值5.5以下或硫化物含量1 225μg/L以上,即能导致中华乌塘鳢产生急性毒理反应,总体表现为红细胞减少,白细胞增加,SOD活力降低,GST活力增强,TP含量升高,T-AOC下降,胁迫强度和持续时间与毒理反应强度呈正相关。【结论】中华乌塘鳢血液生理生化指标对红树林地埋管道生态养殖环境的响应与池塘养殖基本一致,一定浓度硫化物或酸会危害中华乌塘鳢健康状况,硫化物和低pH值不是红树林地埋管道生态养殖的限制因子,但仍是潜在的危害因子。     

Saponins Extracted from Tea (Camellia Sinensis) Flowers Induces Autophagy in Ovarian Cancer Cells

Tea flower saponins (TFS) possess effective anticancer properties. The diversity and complexity of TFS increases the difficulty of their extraction and purification from tea flowers. Here, multiple methods including solvent extraction, microporous resin separation and preparative HPLC separation were used to obtain TFS with a yield of 0.34%. Furthermore, we revealed that TFS induced autophagy—as evidenced by an increase in MDC-positive cell populations and mCherry-LC3B-labeled autolysosomes and an upregulation of LC3II protein levels. 3-MA reversed the decrease in cell viability induced by TFS, showing that TFS induced autophagic cell death. TFS-induced autophagy was not dependent on the Akt/mTOR/p70S6K signaling pathway. TFS-induced autophagy in OVCAR-3 cells was accompanied by ERK pathway activation and reactive oxygen species (ROS) generation. This paper is the first report of TFS-mediated autophagy of ovarian cancer cells. These results provide new insights for future studies of the anti-cancer effects of TFS.