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71.
Elena V. Schmalhausen Vladimir I. Muronetz Natalya K. Nagradova 《Applied biochemistry and biotechnology》2000,88(1-3):211-220
D-Glyceraldehyde-3-phosphate dehydrogenase (GAPDH, EC 1.2.1.12) catalyzes the oxidative phosphorylation of its substrate in
a two-step reaction. Asa result of the first, oxidativestep, the covalent intermediate where in 3-phosphoglyceroyl moiety
is bound to Cys149 of the active center is subjected to nucleophilic attack by inorganic phosphate, but remains resistant
to hydrolytic decomposition. This ensures tight coupling of oxidation with phosphorylation in glycolysis. In this article,
we present the experimental evidence for the conversion of GAPDH into a form capable of performing the reaction in the absence
of inorganic phosphate. The structural basis for this conversion is the oxidation of a cysteine residue (probably Cys 153)
into a sulfenic acid derivative under mild conditions to affect the integrity of the essential Cys 149. As a result, an intram
olecular transfer of 3-phosphoglyceroyl group from the active center Cys 149 to Cys 153 becomes possible with subsequent hydrolysis
of the sulfenyl carboxylate intermediate. 相似文献
72.
The metabolic profiling of kiwifruit (Actinidia deliciosa, Hayward cultivar) aqueous extracts and the water status of entire kiwifruits were monitored over the season (June-December) using nuclear magnetic resonance (NMR) methodologies. The metabolic profiling of aqueous kiwifruit extracts was investigated by means of high field NMR spectroscopy. A large number of water-soluble metabolites were assigned by means of 1D and 2D NMR experiments. The change in the metabolic profiles monitored over the season allowed the kiwifruit development to be investigated. Specific temporal trends of aminoacids, sugars, organic acids and other metabolites were observed.The water status of kiwifruits was monitored directly on the intact fruit measuring the T2 spin-spin relaxation time by means of a portable unilateral NMR instrument, fully non-invasive. Again, clear trends of the relaxation time were observed during the monitoring period.The results show that the monitoring of the metabolic profiling and the monitoring of the water status are two complementary means suitable to have a complete view of the investigated fruit. 相似文献
73.
An effective method for producing methyl 4-bromo-3-hydroxybutyrate enantiomers was developed using an engineered protein. Escherichia coli transformant cells containing a mutant β-keto ester reductase (KER-L54Q) from Penicillium citrinum and a cofactor-regeneration enzyme such as glucose dehydrogenase (GDH) or Leifsonia sp. alcohol dehydrogenase (LSADH) were used to produce methyl (S)-4-bromo-3-hydroxybutyrate from methyl 4-bromo-3-oxobutyrate. On the other hand, the production of methyl (R)-4-bromo-3-hydroxybutyrate was achieved by asymmetric reduction of methyl 4-bromo-3-oxobutyrate with a mutant phenylacetaldehyde reductase (PAR-HAR1) from Rhodococcus sp. ST-10. 相似文献
74.
Potential cycling was used for oxidation of chloropromazine and producing an electroactive redox couples which strongly adsorbed on the electrode surface modified with carbon nanotubes and ionic liquid nanocomposite. The modified electrode shows excellent electrocatalytic activity toward NADH oxidation. The differential pulse voltammetry detection provided high sensitivity, 0.5835 A M?1, low detection limit, 80 nM at concentration range up to 20 μM. An ethanol biosensor was also developed by immobilizing alcohol dehydrogenase enzyme onto nanocomposite. Differential pulse voltammetric detection of ethanol gives linear responses over the concentration range 40 μM–1.5 mM with detection limit 5 μM and sensitivity 1.97 μA mM?1. 相似文献
75.
The conversion of 23-nor-5,20(22)E-choladienic acid-3β-ol and other intermediates of the putative norcholanic acid pathway of cardenolide biosynthesis by recombinant 3β-hydroxysteroid dehydrogenase from Digitalis lanata in dehydrogenation and reduction reactions was investigated. 23-nor-4,20(22)E-choladienic acid-3-one was found to be a substrate of recombinant progesterone 5β-reductases from D. lanata and Arabidopsis thaliana. The role of various substrates in cardenolide biosynthesis is discussed. 相似文献
76.
《Analytical letters》2012,45(13):2305-2316
Abstract A modified spectrophotometric method for serum glutamic-oxaloacetic transaminase (SGOT) assay was developed. A crude cell-free extract from Streptomyces aureofaciens which showed a high level of malate dehydrogenase (MDH) activity (E.C. 1.1.1.37) was used as the enzymatic indicator. The lyophilized microbial preparation was used without previous purification and was quite stable under refrigeration for one year. Serum sample assays using both the method utilizing the crude cell extract and an enzymatic commercial kit showed good correlation. 相似文献
77.
LIANG Wei~ HU Hongying~ GU Xin~ CHE Yuling~ WANG Hui~ GUO Yufeng~ SONG Yudong~ . State Key Laboratory of Freshwater Ecology Biotechnology Institute of Hydrobiology Chinese Academy of Sciences Wuhan Hubei China . Environment Simulation Pollution Control State Key Joint Laboratory Department of Environmental Science Engineering Tsinghua University Beijing China 《武汉大学学报:自然科学英文版》2006,11(3):737-741
0 IntroductionMioclroogincuatlri pernotsce pslsaeys afo vrer wyais tmepwoarttaernt t rroelaetmine nbti- .A widerange of micronutrientsis required byactiva-ted sludge[1-5]. Adequate trace nutrient concentra-tions ,including metal ions and vitamins , are re-quiredtosupport all the genera presentinthe activa-ted sludgetreatment systemfor a diverse communityto survive. The lack of specific nutrients may resultin an unbalanced mixed culture,leading to poor-quality effluent , reduced treatment effic… 相似文献
78.
EffectsofHe-NelaserirradiationonenzymeactivityinmilkingGoats'seminalplasmaYUEWenbin;ZHENGXiaozhong;CUIBaowei;LIKelu(ShanxiAgr... 相似文献
79.
谷氨酸氧化酶发酵液经(HN4)2SO4沉淀后用透析法获得酶粗品。利用GO测定谷氨酸发酵液中谷氨酸的含量与传统的瓦氏法结果一致,相关系数为0.986,相关方程为:y-0.928x+0.127(其中y,x分别表示氧化酶法与瓦氏法测定的值)。且这种方法比瓦氏法方便、简单、分析成本低、耗时少。利用GO法测定血清中谷草转氨酶和谷丙转氨酶的含量,与传统的试剂盒法比较,相关系数分别为0.978、0.984,相关 相似文献
80.
Polymorphisms in genes involved in folate metabolism as maternal risk factors for Down syndrome in China
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Objective: To explore the relationship between genetic polymorphisms in methylenetetrahydrofolate reductase (MTHFR), methionine synthase reductase (MTRR), the central enzymes in folate metabolism that affects DNA methylation and synthesis, and the risk of Down syndrome in China. Methods: Genomic DNA was isolated from the peripheral lymphocytes of 64 mothers of children with Down syndrome and 70 age matched control subjects. Polymerase chain reaction and restriction fragment length polymorphism were used to examine the polymorphisms of MTHFR 677C→T, MTRR 66A→G and the relationship between these genotypes and the risk of Down syndrome was analyzed. Results: The results show that the MTHFR 677C→T polymorphism is more prevalent among mothers of children with Down syndrome than among control mothers, with an odds ratio of 3.78 (95% confidence interval (CI), 1.78-8.47). In addition, the homozygous MTRR 66A→G polymorphism was independently associated with a 5.2-fold increase in estimated risk (95% CI, 1.90-14.22). The combined presence of both polymorphisms was associated with a greater risk of Down syndrome than the presence of either alone, with an odds ratio of 6.0 (95% CI, 2.058-17.496). The two polymorphisms appear to act without a multiplicative interaction. Conclusion: MTHFR and MTRR gene mutation alleles are related to Down syndrome, and CT, TT and GG gene mutation types increase the risk of Down syndrome. 相似文献