铜绿假单胞菌耐药机理

铜绿假单胞菌是耐药性十分突出的一类院内感染条件致病革兰氏阴性细菌．本文从生物被膜、细胞密度依赖式毒力因子、外排系统、基因盒一整合子系统及铜绿假单胞菌噬菌体几方面对其耐药性机理进行了概述，并提出了未来对微生物耐药性研究和应用的一些策略。     

混凝去除铜绿微囊藻(Microcystis aeruginosa)的效果研究

取纯培养条件下的铜绿微囊藻水样，分别用三氯化铁、聚合氯化铝、硫酸铝等3种药剂作混凝去除铜绿微囊藻烧杯试验，结果表明：聚合氯化铝混凝效果优于其他2种药剂，具有药剂投加量少(30～40mg／L)、矾花形成快、矾花沉降性能好、除藻效率高等特点．在聚合氯化铝最佳混凝条件下，投加聚丙烯酰胺助凝试验，发现添加6mg／L的聚丙烯酰胺只使除藻效率在原有基础上提高3％．铜绿微囊藻水样的pH对聚合氯化铝的混凝效果有明显影响，产生最佳混凝的pH值范围为6．0～7．0之间．     

铜绿微囊藻对浮游动物生长繁殖的影响

分离并纯化培养草履虫、绿眼虫及枝角类的大型氵蚤等浮游动物 ,同时培养产毒铜绿微囊藻 .逐日观察、计数稳定期的铜绿微囊藻培养液在不同密度时对不同种类浮游动物生长繁殖的影响 .结果表明 :铜绿微囊藻对不同类别浮游动物的影响因藻类浓度变化而异 ,铜绿微囊藻对草履虫的抑制影响不明显 ;对绿眼虫的生长繁殖能较强抑制 ,当铜绿微囊藻的密度大于 9× 1 0 6 个 /L时 ,绿眼虫几乎无法生存 ;当铜绿微囊藻密度大于 9× 1 0 9个 /L时 ,对大型氵蚤表现出一定程度的抑制     

铜绿微囊藻对UV-B的适应及其种内差异

 在人工UV-B辐照下培养铜绿微囊藻的3个单细胞纯种株系(FACHB905,FACHB85,FACHB69),结果发现:UV-B辐射下藻细胞数目和叶绿素a含量下降,而细胞干重和胞内外多糖含量上升,水溶性和脂溶性色素的吸收光谱改变;铜绿微囊藻对UV-B的适应存在种内差异.     

池塘微囊藻水华的研究

微囊藻水华会造成池塘缺氧或产生有毒物质 ,影响鱼类的生长和生存 .就微囊藻水华的成因进行了研究 ,同时还讨论了微囊藻水华的治理方法     

An ecological study on dynamics of toxic Microcystis blooms in a eutrophic park pond, Wuhan, China

The results of ecological investigation of Microcystis blooms and their toxicity in a eutrophic park pond ( where the annual average of total nitrogen and total phosphorus was 6.1 mg'L^-1 and 1. 79 mg'L^-1. respectively) are presented. The blooms were mainly contributed by M. aeruginosa occurring in the period from May to October when water temperature ranged from 16 to 33 °C . Three remarkable growth peaks of Microcystis during the period were observed with chlorophyll a level of 0. 73 mg*L^-1. 44 mg*L^-1. and 1. 30 mg'L^-1. respectively. The blooms were independent of phosphorus, but highly dependent on ammonium. A level of ammonium of 9. 5 mg'L^-1 could trigger the outbreak of the bloom, while that below 0. 89 mg'L^-1 could inhibit its formation. In other words, ammonium in higher concentrations could promote blooming, while that in lower concentrations could be inhibitory. Microcystis toxicity tended to increase with the blooming process, but the toxic peaks lagged behind their corresponding growth peaks.     

浸没式UV-C技术对铜绿微囊藻生长的抑制效应

以“水华”蓝藻中常见的铜绿微囊藻(Microcystis aeruginosa)为受试藻种，探索一种用于蓝藻“水华”控制的方法，即浸没式UV-C技术．并通过一系列实验，分别从群体、细胞、分子水平，对此技术控制蓝藻生长的抑制效应予以阐释．     

Study on ultrasonic treatment for degradation of Microcystins (MCs)

In recent years, The ecological environment of rivers and lakes have been seriously polluted, and the eutrophication of water bodies has become increasingly prominent, which not only seriously affects the living environment of surrounding residents, but also poses a major threat to the ecological security of water environment. The growth of algae is characterized by short cycle, rapid reproduction and great harmfulness. Conventional algal removal technology is expensive, easy to produce secondary pollution, and difficult to effectively inhibit algae outbreaks, therefore, a new environmental protection technology, ultrasonic algae removal technology, has been put forward. Under the background of ecological environment pollution, in this paper, the effect of ultrasonic technology on degradation of Microcystins (MCs) under different conditions and is investigated. Results show that Microcystins removal rate reaches 81% when Microcystin solution with a concentration of 12.43 mu/L is treated by ultrasound (1200 W) for 5 min; the removal rate of Microcystin reaches 99% after 15 min of ultrasound treatment (1200 W), and almost all of them are removed; no matter wastewater containing Microcystis is treated by ultrasound alone or ultrasound-coagulation method, the levels of Microcystins in the water do not increase. The results also prove that ultrasound can directly destroy the wall and kill algae, inhibit the growth activity of un-killed algae and degrade Microcystins. In addition, the technical principle and application prospect of ultrasonic algae removal instrument in ecological environment are introduced. The paper provided certain direction and theoretical support for the subsequent improvement of ultrasonic algae removal technology.     

Wound Healing,Antimicrobial and Antioxidant Properties of Clinacanthus nutans (Burm.f.) Lindau and Strobilanthes crispus (L.) Blume Extracts

Clinacanthus nutans is known to be an anticancer and antiviral agent, and Strobilanthes crispus has proven to be an antidiuretic and antidiabetic agent. However, there is a high possibility that these plants possess multiple beneficial properties, such as antimicrobial and wound healing properties. This study aims to assess the wound healing, antioxidant, and antimicrobial properties of Clinacanthus nutans and Strobilanthes crispus. The Clinacanthus nutans and Strobilanthes crispus leaves were dried, ground, and extracted with ethanol, acetone, and chloroform through cold maceration. In a modified scratch assay with co-incubation of skin fibroblast and Methicillin-resistant Staphylococcus aureus, Clinacanthus nutans and Strobilanthes crispus extracts were assessed for their wound healing potential, and the antimicrobial activities of Clinacanthus nutans and Strobilanthes crispus extracts were performed on a panel of Gram-positive and Gram-negative bacteria on Mueller–Hinton agar based on a disc diffusion assay. To assess for antioxidant potential, 2,2-diphenyl-1-picrylhydrazyl (DPPH), total phenolic and total flavonoid assays were conducted. In the modified scratch assay, Clinacanthus nutans extracts aided in the wound healing activity while in the presence of MRSA, and Strobilanthes crispus extracts were superior in antimicrobial and wound healing activities. In addition, Strobilanthes crispus extracts were superior to Clinacanthus nutans extracts against Pseudomonas aeruginosa on Mueller–Hinton agar. Acetone-extracted Clinacanthus nutans contained the highest level of antioxidant in comparison with other Clinacanthus nutans extracts.     

苦参与环丙沙星合用对铜绿假单胞菌生物膜的影响

采用体外复制铜绿假单胞菌生物膜模型,研究了苦参水煎液与环丙沙星联合应用对铜绿假单胞菌生物膜的影响及协同杀菌效果,结果显示,1/16、1/4最小抑菌浓度(Minimal Inhibitory Concentration MIC)的苦参水煎液可显著增加1/41、/2 MIC的环丙沙星对铜绿假单胞菌生物膜的抑菌活性,两药合用具有明显的协同作用.