Far infrared-assisted extraction followed by capillary electrophoresis for the determination of bioactive constituents in the leaves of Lycium barbarum Linn.

In this work, a method based on capillary electrophoresis with amperometric detection and far infrared-assisted extraction has been developed for the determination of rutin, gentisic acid, and quercetin in the leaves of Lycium barbarum Linn. The effects of detection potential, irradiation time, and the voltage applied on the infrared generator were investigated to acquire the optimum analysis conditions. The detection electrode was a 300-μm-diameter carbon disc electrode at a detection potential of +0.90 V. The three analytes could be well separated within 12 min in a 40 cm length fused-silica capillary at a separation voltage of 12 kV in a 50 mM borate buffer (pH 9.2). The relation between peak current and analyte concentration was linear over about 3 orders of magnitude with the detection limits (S/N = 3) of 0.31, 0.48, and 0.78 μM for rutin, gentisic acid, and quercetin, respectively. The proposed method has been applied to determine the three bioactive constituents in real plant samples.     

黑果枸杞色素中原花青素含量测定

采用紫外-可见分光光度法测定黑果枸杞色素中的原花青素的含量,黑果枸杞色素中原花青素含量是22g/100g。该方法操作简单、灵敏度高,适宜检测色素中原花青素的含量,为以后的黑果枸杞色素的开发与利用提供理论依据。     

枸杞花药愈伤组织细胞悬浮培养与植株再生

采用枸杞花药进行离体培养,建立细胞系,诱导植株再生,结果在含不同激素的４种培养基上都诱导出愈伤组织,诱导率在１．７％￣１６．９％,愈伤组织在ＭＳ＋２,４－Ｄ０．５ｍｇ／Ｌ的固体培养基上获得大量单细胞,在液体培养基中获得含有大量胚状体的愈伤组织块,收集悬浮培养物转移到ＭＳ＋６ＢＡ０．２ｍｇ／Ｌ的固体培养基上,胚状体能够萌发形成大量绿色小芽,转入生根培养基（ＭＳ＋ＮＡＡ０．２ｍｇ／Ｌ）中,２０ｄ后得到     

Antihyperlipidemic glycosides from the root bark of Lycium chinense

Three new glycosides (1–3), together with six known ones (4–9), were isolated from the root bark of Lycium chinense. Their structures were elucidated on the basis of MS and NMR spectroscopic data. Five compounds (3, 5, 6, 8, and 9) exhibited potent antihyperlipidemic activities in HepG2 cells as assessed by Oil Red O staining and significant inhibition of intracellular triglyceride (TG) levels, whereas two compounds (5 and 9) significantly reduced total cholesterol (TC) levels.     

气质联用法分析枸杞水溶性粗多糖中单糖组成

采用红外、气质联用技术分析了枸杞水溶性多糖中单糖组成及其含量.结果表明,枸杞多糖里含有的单糖包括阿拉伯糖,鼠李糖,木糖,甘露糖,半乳糖,葡萄糖,其含量比例为13.19∶3.41∶2.92∶10.53:18.92:9.82.     

枸杞对家兔红细胞膜磷脂成分及电泳率的影响

采用家兔血细胞和血小板，以薄层色谱扫描法和显微（细胞）电泳法探讨枸杞提取物（ＬｃＭｅ）对家兔红细胞膜磷脂成分及电沪率的影响。结果表明：实验组红细胞膜磷脂成分中ＰＣ显增多，而ＳＭ、ＰＥ都程度不同的减少，特别是ＰＳ含量、ＳＭ／ＰＣ、ＰＳ／ＰＣ比值显减小。血小板及红血细胞的电沪率显高于对照组，ＬｃＭｅ对维持细胞膜的稳态有重要作用。     

枸杞髓部细胞悬浮培养及其高频率植株再生的研究

分离优质枸杞单细胞在含不同激素的４种ＭＳ培养基上都诱导出了愈伤组织，诱导频率５６．７％￣９５．７％；愈伤组织经２￣３次继代培养后，再在液体培养基中进行振荡悬浮培养，建立稳定的单细胞悬浮系。经分化培养、生根培养得到了完整的再生植株。