直观推导式演进特征投影法分辨枸杞类胡萝卜素的异构体

采用高效液相色谱-二极管阵列检测方法,初步分离了枸杞子中的类胡萝卜素。以乙腈-二氯甲烷(体积比为60∶40)为流动相,流速为1.0 mL/min,用C18柱从枸杞类胡萝卜素中分离出7个峰。但由于类胡萝卜素的结构多样性,尤其是顺反异构体的出现使得分离并不完全。利用所得三维数据提供的信息和化学计量学方法直观推导式演进特征投影法(HELP),对其中某些在二维色谱中被定性为单组分的峰进行解析,结果发现原来在二维色谱中被定性为单组分的峰大多是一些多组分峰。以其中第4个峰为例对其进行解析,得到了该类胡萝卜素的同分异构体色谱流出曲线及紫外光谱信息。该方法表明,化学计量学方法与现代分析手段有机结合,大大降低了此类复杂体系对色谱分离的要求,对同分异构体的分析具有一定的借鉴和指导意义。     

细胞呼吸爆发的微量量热学研究

The heat production of Wistar rat polymorphonuclear leukocytes (PMN) was measured by an LKB 2277 Thermal Activity Monitor. When PMN were activited with phorbol-12-myristate13-acetate (PMA), the respiratory burst was recorded by greatly incr eased heat production. Experiment was also carred out in the present of the inhibitor, Total Flavonoids of Lycium Barbarum L. (TFL). The respiratory burst heat production peak was disappeared, but the heat production curve was higher than that of PMA because TFL increased the metabolic activities of PMN.     

“枸杞花粉口服液”对巨噬细胞功能的影响

作者观察了“枸杞花粉口服液”对小鼠腹腔巨噬细胞形态、酸性磷酸酶变化及对酶母细胞的吞噬活性，结果表明：“枸札花粉口服液”可以激活巨噬细胞，吴活化状态ＡＣＰａｓｅ活性增高，对酵母细胞吞噬活性增强，说明“枸杞花粉口服液”可提高机体免疫功能。     

4种蜜源植物花蜜腺比较解剖学研究

荠、酸枣、老瓜头和宁夏枸杞４种植物花蜜腺形态结构各异。荠具４枚颗粒状花托蜜腺；酸枣蜜腺位于花盘表面；老瓜头的花蜜腺位于５个花丝凹糟中，形成蜜腺分泌毛；宁夏枸杞花蜜腺位于子房基部。４种花蜜腺都属结构蜜腺。它们发育早期均无特殊的原始细胞，都是由花器官表层细胞恢复分裂能力，分化形成蜜腺原基，进一步发育成蜜腺。在泌蜜前后，４种蜜腺组织细胞的液泡化程度发生了规律性的变化；荠和宁夏枸杞花蜜腺中多糖物质也发生了同样的变化．     

柴达木盆地产枸杞的资源状况及其栽培繁育研究进展

本文从柴达木盆地产枸杞的生境、资源量及其分布规律,主要种类及其分布,栽培繁育等三个方面,对柴达木枸杞的资源和栽培繁育的研究进展进行了综述,以期为柴达木产枸杞的生物学属性、栽培繁育及其产业化的深入研究提供相应的参考.     

宁夏枸杞花粉形态的扫描电镜观察

首次报道了宁夏枸杞３个品种、一个变种的花粉在扫描电镜下的形态特征．结果表明：供试花粉均为小花粉类型，椭圆形，或近球形，三孔沟，等极，表面有夹带二岐分叉的条状纹饰作纵向排列．具不同条状纹饰和条纹表面的不规则细横纹是供试花粉的区别点．花粉的大小和形状，在供试各品种之间也有不同程度的差异     

白枸杞瘤瘿螨的发生与为害

描述了新疆枸杞园白枸杞瘤瘿螨的形成特征,调查研究了该螨的发生规律、危害及其扩散蔓延方式,提出了防治建议。     

Determination of Taurine in Lycium Barbarum L. and Other Foods by Capillary Electrophoresis with Electrochemical Detection

A method based on capillary electrophoresis (CE) with electrochemical detection (ED) was developed for the determination of taurine in Lycium Barbarum L., LIPOVIYAN beverage and milk powder. The effects of some important factors such as the acidity of the running buffer, separation voltage, injection time, and applied potential to working electrode were investigated. Operated in a wall‐jet configuration, a 300 μm diameter carbon‐disk electrode was used as the working electrode, which exhibits good responses at +1.05 V (vs. SCE) for taurine. Excellent linearity was obtained in the concentration range from 5.0×10^?4 mol/L to 5.0×10^?6 mol/L. The detection limit (S/N=3) was 1.0×10^?7 mol/L. This proposed method has been successfully applied to analyze the actual samples with satisfactory assay results.     

Genetic Transformation of Lycium barbarum L. via A. tumefaciens

A system for transformation and regeneration of Lycium barbarum L. , an important Chinese medical plant, has been established. Young stem segments from Lycium barbarum L. were infected with Agrobacterium tumefaciens C58cl(pGV3850: :ineo1130), and the transformed calli selected from the callus induction medium containing 50 fig/ml kanamycin could regenerate buds on differentiation medium containing 25 μg/ml kanamycin. 30% of the regenerated buds were normal in morphology. The normal buds could develop into whole plantlets after they were transferred to the rooting medium to induce roots. Nopa-line detection, NPT-Ⅱ enzyme activity assay and Southern blotting hybridization indicated that the foreign genes had been integrated into the genome of Lycium barbarum L. and expressed in the plant. In the processes of experiments, it was found that (i) after the pre-processes, the explants which formed callus quickly were easy to transform ; (Ⅱ) the rate of normal regenerated plants from transgenic calli was hig     

Low‐temperature headspace‐trap gas chromatography with mass spectrometry for the determination of trace volatile compounds from the fruit of Lycium barbarum L.

The total saccharides content of Lycium barbarum L. is very high, and a high temperature would result in saccharide decomposition and the emergence of a large amount of water. Moreover, the volatile compounds from the fruit of L. barbarum L. are rather low in concentration. Hence, it is difficult for a conventional headspace method to study the volatile compounds from the fruit of L. barbarum L. Since headspace‐trap gas chromatography with mass spectrometry is an excellent method for trace analysis, a headspace‐trap gas chromatography with mass spectrometry method based on low‐temperature (30°C) enrichment and multiple headspace extraction was developed to explore the volatile compounds from the fruit of L. barbarum L. The headspace of the sample was extracted in 17 cycles at 30°C. Each time, the compounds extracted were concentrated in the trap (Tenax TA and Tenax GR, 1:1). Finally, all the volatile compounds were delivered into the gas chromatograph after thermal desorption. With the method described above, a total of 57 compounds were identified. The identification was completed by mass spectral search, retention index, and accurate mass measurement.