血小板相关抗体及膜糖蛋白检测在免疫性血小板减少症患者诊断中的价值

目的探讨血小板表面血小板相关抗体(血小板相关免疫球蛋白)及血小板膜糖蛋白在免疫性血小板减少症中的诊断及预后评价应用价值.方法采用流式细胞术(FCM)测定了70例血小板减少症患者及20名正常人血小板表面血小板相关免疫球蛋白(PAIgG)及血小板膜糖蛋白(CD61,CD41,GPIIb/IIIa).结果初治30例特发性血小板减少性紫癜(ITP)患者中血小板相关抗体PAIgG在血小板表面阳性百分率均高于正常对照组(P<0.01),血小板膜糖蛋白均低于正常对照组(P<0.01);20例经激素治疗的ITP患者PAIgG与正常对照组相比差异无统计学意义(P>0.01),血小板膜糖蛋白与正常对照组相比差异无统计学意义(P>0.01);继发性血小板减少症患者(慢性再生障碍性贫血、白血病、类风湿关节炎)血小板相关抗体在血小板表面阳性百分率均高于正常对照组(P<0.01),血小板膜糖蛋白均低于正常对照组(P<0.01);20例治疗有效患者治疗后血小板相关抗体较治疗前下降(P<0.01),血小板膜糖蛋白上升,其差异有统计学意义(P<0.01).结论 FCM用于免疫性血小板减少症患者的血小板相关抗体及血小板膜糖蛋白检测,具有灵敏、快速、简便的特点,是适用于临床诊断的新方法,对免疫性血小板减少症的诊断及疗效观察有较好的实用价值.     

糖芯片的检测及应用

糖芯片技术具有样品少、通量高和特异性强等优点,是一种糖组学研究的新的技术平台和强大的分析工具,已经广泛用于糖和蛋白质的特异性作用、酶活性和抑制剂、病毒入侵机理、细菌检测和免疫反应等方面的研究.本文简要介绍了糖芯片的原理、制备和信号的检测技术(荧光标记法、质谱法、SPR法等),分析了糖芯片在各个领域的应用及其发展前景.     

一种新型的制造元设计方案

建立了制造元设计问题的整数规划模型,设计了一种基于疫苗接种的免疫算法,构造了一种基于免疫计算的制造元设计问题解决方案。实验结果表明,本文方案优于已有方案,能够有效地解决大中等规模的制造元设计问题,具有较好的应用价值。     

基于主体网络建模研究溃疡性结肠炎发展过程中的免疫反应动力学

Ulcerative colitis,an inflammatory bowel disease,is a chronic inflammatory disorder that results in ulcers of the colon and rectum without known etiology.Ulcerative colitis causes a huge public health care burden particularly in developed countries.Many studies suggest that ulcerative colitis results from an abnormal immune response against components of commensal microbiota in genetically susceptible individuals.However,understanding of the disease mechanisms at cellular and molecular levels remains largely elusive.In this paper,a network model is developed based on our previous study and computer simulations are performed using an agent-based network modeling to elucidate the dynamics of immune response in ulcerative colitis progression.Our modeling study identifies several important positive feedback loops as a driving force for ulcerative colitis initiation and progression.The results demonstrate that although immune response in ulcerative colitis patients is dominated by anti-inflammatory/regulatory cells such as alternatively activated macrophages and type Ⅱ natural killer T cells,proinflammatory cells including classically activated macrophages,T helper 1 and T helper 17 cells,and their secreted cytokines tumor necrosis factor-α,interleukin-12,interleukin-23,interleukin-17 and interferon-γ remain at certain levels (lower than those in Crohn''s disease,another inflammatory bowel disease).Long-term exposure to these proinflammatory components,causes mucosal tissue damage persistently,leading to ulcerative colitis.Our simulation results are qualitatively in agreement with clinical and laboratory measurements,offering novel insight into the disease mechanisms.     

基于遗传算法的路由选择问题的研究

针对多目标优化问题,应用免疫遗传算法的基本思想,提出了一种求解满足带宽-时延约束多组播路径问题的两层遗传算法。在算法中设计了一种基于节点连接路径的具有树状结构的染色体表示方法及可以实现树状染色体交叉和变异的算子。数值实验结果表明,文中提出的算法可以有效找到多组播路由问题的优化解。     

未来的丙型肝炎疫苗

丙型肝炎病毒（hepatitis C virus,HCV）是丙型肝炎的病原体,是导致输血后肝炎和非甲非乙型肝炎的主要致病因子。HCV感染常导致慢性肝炎、肝硬化和肝细胞癌。HCV在细胞内和动物体内复制能力很差,没有理想的细胞模型和动物模型。丙型肝炎目前尚无疫苗,还有很多病人在等待治疗。研制预防性和治疗性疫苗,以控制HCV感染有着重要意义。经过几代科技工作者的努力和奉献,丙型肝炎疫苗研究取得了重大进展。用核酸疫苗作首次免疫,用多肽疫苗或树突状细胞疫苗作加强免疫,不但有预防作用,而且有治疗效果。核酸疫苗将为改善人类和动物健康作出巨大贡献。     

Upper-lower solutions for nonlinear parabolic systems and their applications

The method of upper-lower solutions for nonlinear parabolic systems without the assumption of quasi-monotonicity is obtained. An application is provided, by using the method developed in this paper, involving the existence of positive solutions to certain time-dependent interaction systems arising in biological and medical sciences. Furthermore, the existence of the ω-limit of given system is studied.     

Fibre-fluorescence immunosensor based on evanescent wave detection

A fibre-optic sensor capable of detecting an immune reaction between fluorescently labelled antigens and antibodies bound at the waveguide surface is described. In a configuration where skew rays were detected, a signal level was found that made detection of a concentration of 10^?9 M antigen possible.     

稚甲鱼二重感染现象分析

本文对１－４月龄的稚甲鱼出现蝗二重感染现象进行了分析，认为是稚甲鱼免疫系统发育不健全，水质的严重污染，致病细菌的数量多，毒力强所致。     

复杂性集类的递归论结构

本文使用间隙语言方法研究了复杂性集类的递归论结构,得到了复杂性集类集合剖分的许多新结果。