基于CNKI数据库的金银花临床应用文献计量分析研究

本文以中国知网(CNKI)数据库收录的1 038篇中文文献为研究对象，采用CiteSpace软件对金银花临床应用方面的研究现状和趋势进行可视化分析，以期为后续研究及强化金银花临床应用提供参考。结果表明，金银花临床研究文献年发文量变化较大(1-57篇/年),2014年发文量最多，为57篇；发文作者整体呈现“小集中、大分散”态势，作者间合作主要在团队内部；发文机构主要集中在山东中医药大学、北京中医药大学等中医药高校及山东中医药大学附属医院、中国中医科学院广安门医院、上海中医药大学附属曙光医院等各大医院；金银花、药理作用、化学成分、绿原酸、中药等关键词在知识图谱中具有重要作用。金银花临床应用研究大致可分为4个阶段：第一个阶段，金银花、清热解毒、生甘草等关键词是研究热点；第二个阶段集中在清热解毒应用及配伍使用方面；第三个阶段除原有研究外，还注重质量控制、中药制剂研发等；第四个阶段，研究向用药规律、关联规则、数据挖掘、免疫功能、免疫调节等方向延伸，这些也是未来的研究热点。综上所述，金银花临床应用处于持续研究中，其功能不断扩展，必将在更多的疾病防治中发挥更大的作用。金银花后续诸多研究集中在数据挖掘...     

Isolation and purification of high polar glycosides from aerial parts of Gynostemma pentaphyllum (Thunb.) Makino by linear gradient counter-current chromatography coupled with inner-recycling mode

Gynostemma pentaphyllum (Thunb.) Makino represents the popular health food and supplemental product with broad pharmacological activities. The highly polar glycosides, including flavonoids and saponins, are major effective active components that contain diverse sugar positions and quantities, which result in diverse chemical polarities, making it challenging to separate and isolate these components. The present work described the rapid and efficient linear gradient counter-current chromatography to preparatively separate glycosides from aboveground parts of G. pentaphyllum. Besides, the ethyl acetate and n-butanol binary mobile phases were achieved through adjusting associated proportions. Six glycosides, including quercetin-3-O-neohesperidoside ( 1 ), kaempferol-3-O-robinobioside ( 2 ), kaempferol-3-O-neohesperidoside ( 3 ), gypenoside LVI ( 4 ), ginsenoside Rb3 ( 5 ), and gypenoside XLVI ( 6 ), were isolated at the purities greater than 98%. Moreover, electrospray ionization mass spectrometry and nuclear magnetic resonance tandem mass spectrometry were conducted for structural identification. According to our findings, the established linear gradient counter-current chromatography was an efficient approach to separate the highly polar glycosides from aboveground parts of G. pentaphyllum. Our proposed strategy can be used to separate active compounds from other complex natural products.