石荠苧不同药用部位总黄酮及微量元素含量的测定

采用分光光度法测定了石荠芋不同药用部位总黄酮的含量,采用电感耦合等离子发射光谱法测定了其中微量元素的含量。结果表明,叶中总黄酮含量最高,根次之,茎最少;根、茎、叶中均含有Cu、Fe、Mn、Zn、Co等多种人体必需的微量元素,其中Fe含量最高,且叶中明显高于根、茎;3个部位均未检出有害重金属As,而Hg、Pb含量均未超标。该结果可为石荠芋不同药用部位的利用和药材的开发提供科学依据。     

鱼腥草总黄酮的提取及其方法对比

采用水提法、有机溶剂提取法、索式抽提法、超声波法等4种方法分别提取鱼腥草不同部位（根、茎、叶及全草）的黄酮,结果表明：索式提取法为鱼腥草总黄酮的最佳提取方法,鱼腥草不同部位的总黄酮含量由高到低依次为叶、全草、茎、根.     

Polycyclic Phenol Derivatives from the Leaves of Spermacoce latifolia and Their Antibacterial and α-Glucosidase Inhibitory Activity

Three new polycyclic phenol derivatives, 2-acetyl-4-hydroxy-6H-furo [2,3-g]chromen-6-one (1), 2-(1′,2′-dihydroxypropan-2′-yl)-4-hydroxy-6H-furo [2,3-g][1]benzopyran-6-one (2) and 3,8,10-trihydroxy-4,9-dimethoxy-6H-benzo[c]chromen-6-one (8), along with seven known ones (3–7, 9 and 10) were isolated for the first time from the leaves of Spermacoce latifolia. Their structures were determined by spectroscopic analysis and comparison with literature-reported data. These compounds were tested for their in vitro antibacterial activity against four Gram-(+) bacteria: Staphyloccocus aureus (SA), methicillin-resistant Staphylococcus aureus (MRSA), Bacillus cereus (BC), Bacillus subtilis (BS), and the Gram-(−) bacterium Escherichia coli. Compounds 1, 2, 5 and 8 showed antibacterial activity toward SA, BC and BS with MIC values ranging from 7.8 to 62.5 µg/mL, but they were inactive to MRSA. Compound 4 not only showed the best antibacterial activity against SA, BC and BS, but it further displayed significant antibacterial activity against MRSA (MIC 1.95 µg/mL) even stronger than vancomycin (MIC 3.9 µg/mL). No compounds showed inhibitory activity toward E. coli. Further bioassay indicated that compounds 1, 4, 5, 6, 8 and 9 showed in vitro α-glucosidase inhibitory activity, among which compound 9 displayed the best α-glucosidase inhibitory activity with IC₅₀ value (0.026 mM) about 15-fold stronger than the reference compound acarbose (IC₅₀ 0.408 mM). These results suggested that compounds 4, 8 and 9 were potentially highly valuable compounds worthy of consideration to be further developed as an effective anti-MRSA agent or effective α-glucosidase inhibitors, respectively. In addition, the obtained data also supported that S. latifolia was rich in structurally diverse bioactive compounds worthy of further investigation, at least in searching for potential antibiotics and α-glucosidase inhibitors.     

淮山水溶性多糖的提取与高效液相色谱分离的研究

采用正交实验法探讨淮山水溶性多糖提取工艺条件，结果表明，水提多糖最佳条件为料液比1：8，70℃下浸提3h一次，当浸提液浓缩6倍，可得到多糖的最高沉降率；碱提淮山多糖最佳条件为料液比1：10，提取时间8个小时，NaOH浓度1.25％．同时，用高效液相色谱检测碱提粗多糖可能含有鼠李糖、阿拉伯糖、果糖、葡萄糖和半乳糖．     

不同因子对“金丰一号”金银花生根的影响

研究了基本培养基,IBA,活性炭和蔗糖对“金丰一号”金银花生根的影响,结果表明:基本培养基为1/2 MS时,生根效果较佳;IBA 3 mg·L-1,时,试管苗生长较好,生根也快;从节约成本出发,蔗糖的浓度为15 g·L-1对生根较为有利.即适于生根的培养基为1/2 MS+IBA 3 mg·L-1+0.2 g·L-1活性...     

鱼腥草叶总黄酮含量的测定及鉴别

以芦丁为标样,选择测定波长为502nm,用分光光度法测定鱼腥草叶总黄酮含量,结果表明:芦丁浓度在8.12~48.72μg/mL范围内,吸光度和浓度呈良好的线性关系(R2=0.9998),测定的平均回收率为99.4%,精密度试验RSD=0.49%,重现性试验RSD=2.07%。该方法精密度高,结果稳定可靠。此外用纸色谱和显色反应对鱼腥草叶黄酮作了初步鉴别。     

Elsholtzia ciliata (Thunb.) Hyland: A Review of Phytochemistry and Pharmacology

In this paper, the confusion of the sources of medicinal materials was briefly expounded, and the differences among the varieties were pointed out. At the same time, the chemical components and pharmacological properties of Elsholtzia ciliata (Thunb.) Hyland (E. ciliata) were reviewed. The structures of 352 compounds that have been identified are listed. These mainly include flavonoids, terpenoids, phenylpropanoids, alkaloids, and other chemical components. They have antioxidant, anti-inflammatory, antimicrobial, insecticidal, antiviral, hypolipidemic, hypoglycemic, analgesic, antiarrhythmic, antitumor, antiacetylcholinesterase, and immunoregulator activities. At present, there are many researches using essential oil and alcohol extract, and the researches on antioxidant, anti-inflammatory, anti-microbial, and other pharmacological activities are relatively mature. This paper aims to summarize the existing research, update the research progress regarding the phytochemicals and pharmacology of E. ciliate, and to provide convenience for subsequent research.     

大叶苦丁茶抗氧化活性研究进展

大叶苦丁茶是我国南方一种药食两用的传统饮品与药材,也是市场上广泛流通、被普遍当作“苦丁茶正品”的别样茶(non-Camellia teas),具有清热解毒、抗氧化、抗炎、止血凉血、抗菌等多种功效,其次生代谢物质化学成分复杂,主要包括多酚、多糖、黄酮、三萜等多种活性成分。大叶苦丁茶活性成分是天然的抗氧化剂,具有绿色、无毒、高效等特点,在食用油脂储藏、食品保鲜、化妆品、保健品以及抗氧化药物等方面具有良好的应用前景。本文以大叶苦丁茶化学成分的抗氧化作用为主线,综述大叶苦丁茶中具有代表性的多酚、多糖、黄酮、三萜等化学成分的含量、组成及其抗氧化作用,探讨大叶苦丁茶的应用并对其进行展望,拟为大叶苦丁茶的开发与利用提供参考。     

宜兴百合的组织培养和快速繁殖

以组织培养获得的宜兴百合小鳞茎为试验材料,研究了不同基本培养基、NAA与6-BA的配比、矮壮素（CCC）、2,4-D、活性炭、蔗糖及悬浮培养方法对小鳞茎增殖和膨大的影响．结果表明,最佳诱导培养基为MS培养基,有利于提高分化率的最佳配方为：MS＋0．15mg／LNAA＋2．0mg／L6-BA,小鳞茎膨大增重的最佳培养基为：MS＋0．15mg／LNAA＋2．0mg／L6-BA＋0.1mg／L CCC＋6—8％蔗糖,培养方式为液体振荡培养优于固体培养．