连翘的组织培养研究

通过组织培养,介绍了连翘的培养方法,并对结果进行了分析,探讨了不同条件下对培养结果的影响。     

石杉碱甲的含量测定方法及在蛇足石杉不同产地居群中的含量比较

采用Waters 1525-2487高效液相色谱仪,测定了产自浙江遂昌大西坑、遂昌九龙山、磐安大盘山、庆元百山祖、临安西天目山、金华南山、金华北山和福建武夷山等8个产地居群蛇足石杉(茎叶)中石杉碱甲的含量.结果发现,磐安大盘山居群蛇足石杉的石杉碱甲含量最高,百山祖和天目山居群的含量最低.因此,人工栽培时可以考虑以磐安大盘山产的蛇足石杉作为种源,并注意以该居群生长环境条件作为栽培的参数.     

Complete assignments of (1)H and (13)C NMR spectroscopic data for two new triterpenoid saponins from Ilex pernyi

Ilexpernoside A and ilexpernoside B, two new pentacyclic C(4)-nortriterpenoid saponins, were isolated from the leaves of Ilex pernyi Franch. Their chemical structures were determined by MS, NMR spectroscopy and chemical analysis. Complete assignments of the (1)H and (13)C NMR spectroscopic data were achieved by 1D and 2D NMR experiments (HSQC, HMBC, (1)H-(1)H COSY and NOESY).     

景天属三种植物药不同提取部位及总黄酮抗肿瘤作用研究

利用MTT法检测景天属三种植物药垂盆草、佛甲草及凹叶景天的乙酸乙酯和正丁醇提取部位及总黄酮提取物对人肝癌细胞株HepG2、人食管癌细胞株EC109及人结肠癌细胞株SW480的体外增值抑制作用.结果表明,三种植物药的乙酸乙酯和正丁醇提取部位及总黄酮提取物都有显著的抗肿瘤作用,且各植物药总黄酮提取物的抗肿瘤活性最强.     

Comprehensive analysis of maté (Ilex paraguariensis) compounds: development of chemical strategies for matesaponin analysis by mass spectrometry

Ilex paraguariensis (maté) is an important plant from southern Brazil, Paraguay, Uruguay and Argentina, where its leaves are widely used in hot or cold beverages. We now present a comprehensive ULPC-PDA-MS analysis of the compounds found in its leaves. The analysis was rapid, less than 13 min and many compounds were identified, among these, the chlorogenic acid series, and other quinic acid derivatives, xanthines, and several saponins, many of which have not been previously described, including many isomers. The isomerism could occur in the aglycone moiety, namely ursolic or oleanolic acid, or in the carbohydrate moiety. Additionally, many acetylated saponins were detected. The structure of known and novel saponins was determined using per-O-methylation with ESI-MS analysis, as well as with GC-MS of their partially O-methylated alditol acetate (PMAA) derivatives, along with ESI-MS analysis of the O-isopropylidene (IPP) derivatives provided fundamental information on interglycosidic linkages, avoiding the purification steps.     

Rapid quantification of four major bioactive alkaloids in Corydalis decumbens (Thunb.) Pers. by pressurised liquid extraction combined with liquid chromatography-triple quadrupole linear ion trap mass spectrometry

A new method based on pressurised liquid extraction (PLE) followed by liquid chromatography-triple quadrupole linear ion trap mass spectrometry (LC-QTrap-MS) analysis has been developed for the identification and quantification of four major alkaloids in extracts of Corydalis decumbens (Thunb.) Pers. PLE extractions were performed using 90% ethanol; temperature was set at 100 °C and pressure at 1500 psi. HPLC analysis was performed on a Waters XBridge™ C₁₈ column (150 mm × 2.1 mm i.d., 3.5 μm) eluted by a mobile phase of acetonitrile and 0.2% acetic acid. Data acquisition was carried out in multiple reaction monitoring transitions (MRMs) mode, monitoring two MRM transitions to ensure an accurate identification of target compounds in the samples. Additional identification and confirmation of target compounds were performed using the enhanced product ion modus (EPI) of the linear ion trap. The novel LC-QTrap-MS platform offers the best sensitivity and specificity for characterization and quantitative determination of the four alkaloids in C. decumbens (Thunb.) Pers. and fulfils the quality criteria for routine laboratory application.     

超高效液相色谱-四极杆串联飞行时间质谱分析黄酮类化合物及小毛茛茎叶的成分

为了探索液相色谱-质谱联用(LC-MS)技术在快速识别中药及天然产物成分中的应用,以黄酮对照品为研究前体,药用植物小毛茛为研究对象,采用超高效液相色谱/二极管阵列检测器-电喷雾四极杆串联飞行时间质谱(UPLC/DAD-ESI/Q-TOF MS)分析了黄酮类化合物同系物及同分异构体的色谱、质谱特性。结果显示:黄酮氧苷和黄酮碳苷的紫外吸收光谱及二级质谱具有显著性差异,糖苷化位置同保留时间、二级质谱碎片及相对丰度具有相关性。将该方法应用于小毛茛茎叶醇提液的分析,结合其酸水解液的分析,解析了22个黄酮醇糖苷和3个苷元。方法简便,具有可操作性。     

Three New 24‐Nortriterpenoids from the Roots of Ilex asprella

Asprellols A–C ( 1 – 3 , resp.), three new 24‐nortriterpenoids, were isolated from the CHCl₃‐soluble fraction of 95% EtOH extract of the roots of Ilex asprella, together with a known nortriterpenoid. The structures of the new compounds were elucidated as 2,6β,20β‐trihydroxy‐3‐oxo‐11α,12α‐epoxy‐24‐norursa‐1,4‐dien‐28,13β‐olide ( 1 ), 2,6β‐dihydroxy‐3‐oxo‐11α,12α‐epoxy‐24‐norursa‐1,4,20(30)‐trien‐28,13β‐olide ( 2 ), and 2,6β‐dihydroxy‐3‐oxo‐11α,12α‐epoxy‐24‐noroleana‐1,4‐dien‐28,13β‐olide ( 3 ) on the basis of spectroscopic analyses.     

浙江省野生牛奶子资源调查

对利用价值较高的野生植物资源牛奶子（Elaeagnus umbellata Thunb.）在浙江省的分布进行了实地调查。结果表明,牛奶子在浙江省各地分布较为广泛,但是由于开荒造田、乱砍滥伐、生态自身竞争等原因,很多地区的牛奶子种质资源遭受严重破坏,个别地区现存数量急剧下降。对浙江省野生牛奶子种质资源的保护、扩增以及合理开发利用等相关研究亟待进一步加强。     

苦丁茶药材有效成分动态积累研究

【目的】研究不同月份苦丁茶(Ilex kudingcha.)药材中有效成分熊果酸和齐墩果酸含量变化的动态积累。【方法】采用反相高效液相色谱法测定熊果酸和齐墩果酸在苦丁茶药材中的含量。【结果】当年10月至次年2月份苦丁茶样品中熊果酸和齐墩果酸的含量分别为1.12%~1.47%及0.16%~0.21%,3~9月份样品中熊果酸和齐墩果酸的含量分别为0.87%~1.21%及0.13%~0.18%。【结论】苦丁茶药材的最佳采收期应为当年10月至次年2月份。