不同山药种质资源的腺苷含量比较研究

目的为了解山药资源腺苷含量情况,进一步对该部分资源的腺苷含量进行分析。方法采用高效液相色谱法测定不同种质资源山药腺苷含量。结果不同种质薯蓣腺苷含量差异极显著,其中腺苷含量最高的是山东兖州的山药(0.199%);最低是河南焦作的山药(0.019%);总平均含量为0.095%。结论不同种质来源的山药腺苷含量差异较大,提示临床用药应注意不同种质来源,同时也为薯蓣的引种提供依据。     

Rapid quantification of four major bioactive alkaloids in Corydalis decumbens (Thunb.) Pers. by pressurised liquid extraction combined with liquid chromatography-triple quadrupole linear ion trap mass spectrometry

A new method based on pressurised liquid extraction (PLE) followed by liquid chromatography-triple quadrupole linear ion trap mass spectrometry (LC-QTrap-MS) analysis has been developed for the identification and quantification of four major alkaloids in extracts of Corydalis decumbens (Thunb.) Pers. PLE extractions were performed using 90% ethanol; temperature was set at 100 °C and pressure at 1500 psi. HPLC analysis was performed on a Waters XBridge™ C₁₈ column (150 mm × 2.1 mm i.d., 3.5 μm) eluted by a mobile phase of acetonitrile and 0.2% acetic acid. Data acquisition was carried out in multiple reaction monitoring transitions (MRMs) mode, monitoring two MRM transitions to ensure an accurate identification of target compounds in the samples. Additional identification and confirmation of target compounds were performed using the enhanced product ion modus (EPI) of the linear ion trap. The novel LC-QTrap-MS platform offers the best sensitivity and specificity for characterization and quantitative determination of the four alkaloids in C. decumbens (Thunb.) Pers. and fulfils the quality criteria for routine laboratory application.     

苦丁茶的微量元素测定及其保健功能初探

用原子吸收光谱法测定了苦丁茶中Zn,Cu,Mn,Fe,Ca,Mg,Sr,Co,Li,Rb,K,Ni的含量，发现其中Zn,Mn,Cu,Mg,K,Rb,Fe,Li的含量比其他茶高得多。并根据测定结果，初步讨论了苦丁茶的保健功能。     

大叶黄杨叶、茎、果挥发油成分及抗病毒作用

研究了大叶黄杨叶、茎、果挥发油的化学成分及抗病毒活性。 采用超临界二氧化碳萃取,应用气相色谱-质谱联用(GC-MS) 法鉴定挥发油化学成分,考察体外抗病毒作用。 共鉴定133个化合物,大叶黄杨叶挥发油中主要有2-乙氧丙烷(41.92%)、(E)-2-己烯-1-醇(17.8%)、 (E)-香叶醇(7.86%)、甲基环己烷(6.60%)等;大叶黄杨茎挥发油中主要有甲氧基苯基肟(33.10%)、二十八烷(14.34%)、α-甲基-α-[4-甲基-3-戊烯基]环氧乙烷甲醇(12.48%)、甲苯(11.88%)、二十一烷(7.74%) 等;大叶黄杨果挥发油中主要有苯甲醛(15.52%)、甲苯(15.03%)、甲基环己烷(14.76%)、(Z)-3-己烯-1-醇(10.98%)等。 大叶黄杨叶、茎、果的环己烷、乙醚萃取挥发油对特定病毒有显著抑制效果。 大叶黄杨叶、茎、果中挥发油萃取部位成分差异明显,有特定抗病毒活性。     

Complete assignments of (1)H and (13)C NMR spectroscopic data for two new triterpenoid saponins from Ilex pernyi

Ilexpernoside A and ilexpernoside B, two new pentacyclic C(4)-nortriterpenoid saponins, were isolated from the leaves of Ilex pernyi Franch. Their chemical structures were determined by MS, NMR spectroscopy and chemical analysis. Complete assignments of the (1)H and (13)C NMR spectroscopic data were achieved by 1D and 2D NMR experiments (HSQC, HMBC, (1)H-(1)H COSY and NOESY).     

Biological characteristics of Edgeworthia tomentosa (Thunb.) Nakai flowers and antimicrobial properties of their essential oils

Edgeworthia tomentosa (Thunb.) Nakai belongs to Thymelaeaceae family, its alabastrum is used as the traditional Chinese medicine ‘Buddleja Officinalis Maxim’. The present study was to elucidate the ultrastructure characteristics of the flower, the phytochemical composition of the aroma essential oils (EOs) and the relevant antimicrobial properties. There were exclusive characters of calyx, ovule, anther and pollen grain of the flowers under scanning electron microscopy. A total of 40 phytochemical components representing 98% of the EOs were successfully identified: monoterpenes and sesquiterpenes were the dominant terpenoids according to Kovats retention index and MS database. EOs exhibited a broad spectrum antimicrobial activity against Gram-positive and Gram-negative bacteria, the best bacteriostatic effect was against Diplococcus pneumonia with MIC and MBC values ranging between 7.8 and 62.5 and 26.0–71.0 μg/mL, respectively. These results demonstrate that the surface microscopic morphological characteristics of Edgeworthia chrysantha Lindl. flowers, are full-scale chemical composition and antimicrobial properties of the EOs.     

超高效液相色谱-四极杆串联飞行时间质谱分析黄酮类化合物及小毛茛茎叶的成分

为了探索液相色谱-质谱联用(LC-MS)技术在快速识别中药及天然产物成分中的应用,以黄酮对照品为研究前体,药用植物小毛茛为研究对象,采用超高效液相色谱/二极管阵列检测器-电喷雾四极杆串联飞行时间质谱(UPLC/DAD-ESI/Q-TOF MS)分析了黄酮类化合物同系物及同分异构体的色谱、质谱特性。结果显示:黄酮氧苷和黄酮碳苷的紫外吸收光谱及二级质谱具有显著性差异,糖苷化位置同保留时间、二级质谱碎片及相对丰度具有相关性。将该方法应用于小毛茛茎叶醇提液的分析,结合其酸水解液的分析,解析了22个黄酮醇糖苷和3个苷元。方法简便,具有可操作性。     

石荠苧不同药用部位总黄酮及微量元素含量的测定

采用分光光度法测定了石荠芋不同药用部位总黄酮的含量,采用电感耦合等离子发射光谱法测定了其中微量元素的含量。结果表明,叶中总黄酮含量最高,根次之,茎最少;根、茎、叶中均含有Cu、Fe、Mn、Zn、Co等多种人体必需的微量元素,其中Fe含量最高,且叶中明显高于根、茎;3个部位均未检出有害重金属As,而Hg、Pb含量均未超标。该结果可为石荠芋不同药用部位的利用和药材的开发提供科学依据。     

溶剂浮选-HPLC法测定茵陈中滨蒿内酯的研究

摘要：建立茵陈中滨蒿内酯的溶剂浮选分离富集方法。方法 考察了浮选溶剂、氮气流速、试液 pH、浮选时间及电解质 NaCl 等因素对浮选效果的影响,优选出最佳浮选条件,并由高效液相色谱测定其含量。结果 对最佳条件下的浮选效果进行了评价。结论 加标回收率92.31 ~ 99.97 %; RSD = 3.20 %.溶剂浮选分离富集方法可行。     

化学发光法测定几种怀山药的抗自由基活性

用不同溶剂将三种怀山药分成极性不同的部位 ,并用鲁米诺—H2 O2 —CuSO4 化学发光体系测定天然产物抗自由基活性的方法检测了这些部位的抗自由基活性 ,比较了不同品种怀山药的质量差异 ,所得结果与传统经验相吻合。