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281.
Voltammetric behaviour and determination of moxifloxacin in pharmaceutical products and human plasma
Erk N 《Analytical and bioanalytical chemistry》2004,378(5):1351-1356
The oxidative behaviour of moxifloxacin was studied at a glassy carbon electrode in different buffer systems using cyclic, differential pulse, and Osteryoung square-wave voltammetry. The oxidation process was shown to be irreversible over the entire pH range studied (2.0–10.0) and was diffusion-controlled. The methods were performed in Britton–Robinson buffer and the corresponding calibration graphs were constructed and statistical data were evaluated. When the proposed methods were applied at pH 6.0 linearity was achieved from 4.4×10–7 to 1.0×10–5 mol L–1. Applicability to tablets and human plasma analysis was illustrated. Furthermore, a high-performance liquid chromatographic method with diode-array detection was developed. A calibration graph was established from 4.0×10–6 to 5.0×10–5 mol L–1 moxifloxacin. The described methods were successfully employed with high precision and accuracy for estimation of the total drug content of human plasma and for pharmaceutical dosage forms of moxifloxacin. 相似文献
282.
Martínez-Pla JJ Martín-Biosca Y Sagrado S Villanueva-Camañas RM Medina-Hernández MJ 《Journal of chromatography. A》2004,1048(1):111-118
Capillary electrophoresis (CE) is a powerful technique for enantiomer separations due to its intrinsic high separation efficiencies, speed of analysis, low reagent consumption and small sample requirements. However, some chiral selectors present strong background UV absorption providing high detection limits. The present paper deals with the application of the partial-filling technique to the separation of bupivacaine enantiomers by capillary electrophoresis using human serum albumin (HSA) as chiral selector. In this procedure the cationic surfactant cetyltrimethylammonium bromide (CTAB) was used as a dinamic capillary coating in order to reduce the electro-osmotic flow and detect both bupivacaine enantiomers out of the chiral selector plug. Several experimental conditions such as CTAB concentration, pH, HSA concentration and plug length, background electrolyte concentration, temperature and voltage were studied. Under the selected conditions it is possible to detect the separated enantiomers out of the HSA plug in less than 4 min using 50 mM Tris pH 8 as background electrolyte with 50 microM CTAB, at 30 degrees C and using a separation voltage of 25 kV. The proposed methodology was then validated for analytical purposes and applied to the analysis of pharmaceutical preparations commercially available. The results obtained with the proposed methodology were in good agreement with those declared by the manufacturers. The simplicity, sample throughput, accuracy, reproducibility and low cost of the proposed method make it suitable for the control of the enantiomeric composition of bupivacaine in pharmaceuticals. 相似文献
283.
血浆样品经0.01mol·L-1盐酸和无水乙醇浸取,在碱性条件下用氯仿萃取,GG-MS-选择离子测量法测定,外标法定量。在选定的色谱条件下,盐酸克仑特罗的检出限为0.05ng,线性范围为0.1-100ng,方法的平均回收率为95.6%,RSD小于6.82%(n=6)。该法毋需衍生化,基质无干扰,简便可靠,适用于生物材料中克仑特罗的快速分析。 相似文献
284.
285.
A total of seven pesticides and eight alkylphenols were monitored using this method for the determination of their trace levels in human cord blood. The pesticides are lindane, diazinon, α-endosulfan, β-endosulfan, endosulfan sulfate, chlorpyrifos and endrin; while the alkylphenols are 4-n-butylphenol, 4-n-pentylphenol, 4-n-hexylphenol, 4-t-octylphenol, 4-n-heptylphenol, nonylphenol, 4-n-octylphenol and bisphenol A. The pesticides and alkylphenols in the cord blood samples were extracted with solid phase extraction IST C18 cartridges and analyzed by selected ion monitoring mode using quadrapole detector in Shimadzu QP-5000 gas chromatograph-mass spectrometer. Trace levels of pesticide and alkylphenols in the range of non-detectable to 15.17 ng ml−1, were detected in the human cord blood samples. This technique of monitoring the levels of endocrine-disruptors in blood samples is consistent, reliable and cost effective while reducing wastage of time and solvents. 相似文献
286.
应用单细胞微凝胶电泳技术研究氯化镉对人血淋巴细胞DNA的损伤效应 总被引:2,自引:0,他引:2
孟紫强 《广东微量元素科学》1998,5(6):4-8
详细介绍了单细胞微凝胶电泳(SCG)技术的原理和操作过程,并应用该技术研究了氯化镉(CdCl2)对人血淋巴细胞DNA的损伤效应,结果表明,CdCl2能引起细胞DNA迁移长度增加,且呈显著的剂量效应关系,对未处理对照细胞的DNA迁移的原因及SCG实验操作过程中应注意的事项也进行了讨论。 相似文献
287.
Hege Rebecka Johansen Christian Thorstensen Tyge Greibrokk Georg Becher 《Journal of separation science》1993,16(3):148-152
On-line coupled supercritical fluid extraction and gas chromatography (SFE-GC) has been utilized for the determination of PCBs and other organochlorine compounds in human milk and blood serum. The procedure involved preconcentration of the sample on C18-silica sorbent in an extraction cell: after precipitation of the proteins up to 20 ml of human milk was concentrated on 0.5 g of sorbent. Serum (up to 5 ml) was applied to the C18 material without pretreatment. Basic alumina was utilized as a selective adsorbent for lipids in the on-line SFE-GC system. The method was used to analyze milk and serum spiked with 0.5 and 10 ng of Aroclor 1260 and the results compared with those obtained by liquid–liquid extraction of serum. 相似文献
288.
微量元素对胎儿发育影响的临床研究 总被引:6,自引:0,他引:6
为了解小于胎龄儿的血锌、铜、铅的变化 ,对 3 8例小于胎龄儿 (其中 1 2例有先天缺陷 )进行了血锌、铜、铅三种微量元素检测 ,并与 3 1例健康足月适于胎龄儿比较。结果表明 ,3 8例小于胎龄儿血锌、铜、铅结果分别为 49 4± 1 9 88μmol/L、 1 0 7± 3 3 μmol/L、 1 5± 0 83 μmol/L ;与正常对照组 (分别为 62 3 9± 1 5 2 9μmol/L、 1 1 0 2± 1 73 μmol/L、 0 82± 0 3 9μmol/L)比较 ,血锌明显低于正常组 ,t=-2 88,P <0 0 1 ;血铅明显高于对照组 ,t =4 6,P <0 0 1 ,有显著性差异。两组血铜无明显差异。小于胎龄儿血锌降低 ,血铅增高 ,而且其先天性缺陷者占3 5 .5 8% ,证明胎儿的低锌高铅状态对其生长发育有重要影响。 相似文献
289.
微量元素锌促进儿童生长发育临床研究 总被引:1,自引:0,他引:1
报道以微量元素锌治疗缺锌患儿118例并与114例作对照观察.结果发现,衡量生长发育与营养的敏感指标:身高、体重、血清白蛋白及血色素等参数,治疗组比对照组都显著提高(P值<0.01).证明锌制剂对缺锌儿童有显著的促进生长发育及改善营养状态作用,作者并对锌的作用机理作了论述. 相似文献
290.
Thermodynamics of the interaction between Ni2+ and human growth hormone (hGH) were determined at 27 °C in Nail solution by isothermal titration calorimetry. A new method to predict protein penetration and the effect of metal ions on the stability of proteins is introduced. The new solvation model was used to reproduce the enthalpies of Ni2+-hGH interaction over the whole range of Ni2+ concentrations. The solvation parameters recovered from the new equation, attributed to the structural change of hGH and its biological activity. 相似文献