多维色谱串联质谱分析肝癌患者血浆中的低丰度差异蛋白

建立了分析肝癌患者血浆中低丰度差异蛋白的新的多维色谱串联质谱方法.以5例肝细胞癌(HCC)患者血浆为研究对象,选用免疫亲和色谱Agilent MARS Spin Cartridge去除血浆中6种主要高丰度蛋白;离线RP-HPLC预分级血浆低丰度蛋白并收集差异表达蛋白组;液相色谱-芯片(HPLC-CHIP,包含一支纳升级Zorbax 300SB-C18富集柱和一支纳升级Zorbax 300SB-C18分析柱)富集、分离差异表达蛋白组胰酶酶解肽混合物,线性离子阱质谱在线分析,以Spectrum Mill MS Proteomics Workbench自动分析MS和MS/MS数据后在UniProtKB/SWISS-PORT数据库中进行搜索,鉴定得到27种差异蛋白,其中23种蛋白与各种疾病或肿瘤相关,IMP3、ARNT2和GRIP1可能成为诊断HCC的潜在生物标志物.     

Metabolomic profiling of human urine in hepatocellular carcinoma patients using gas chromatography/mass spectrometry

With the technique of metabolomics, gas chromatography/mass spectrometry (GC/MS), urine or serum metabolites can be assayed to explore disease biomarkers. In this work, we present a metabolomic method to investigate the urinary metabolic difference between hepatocellular carcinoma (HCC, n = 20) male patients and normal male subjects (n = 20). The urinary endogenous metabolome was assayed using chemical derivatization followed by GC/MS. After GC/MS analysis, 103 metabolites were detected, of which 66 were annotated as known compounds. By a two sample t-test statistics with p < 0.05, 18 metabolites were shown to be significantly different between the HCC and control groups. A diagnostic model was constructed with a combination of 18 marker metabolites or together with alphafetoprotein, using principal component analysis and receiver-operator characteristic curves. The multivariate statistics of the diagnostic model yielded a separation between the two groups with an area under the curve value of 0.9275. This non-invasive technique of identifying HCC biomarkers from urine may have clinical utility.     

Evaluation of lung tumor perfusion by dynamic contrast-enhanced MRI

The characterization of solid pulmonary lesions with imaging methods remains a diagnostic challenge. The aim of this study was to correlate kinetic parameters of dynamic perfusion magnetic resonance imaging (MRI) with histological tumor classification. Dynamic contrast-enhanced MRI of 31 patients with pulmonary masses (five benign lesions, 26 malignant tumors) was acquired in the tumor areas every 20 s for a mean duration of 124 s. Contrast uptake (CU) was measured by signal analysis in regions of interest (ROIs). The beginning and duration of CU, maximum CU (MCU, % of baseline), maximum contrast upslope (%/s) and the delay to the maximum contrast upslope (s) were calculated. All lesions were classified histologically. The beginning of CU correlated significantly with the MCU delay in all lesions (P=.033). The frequency of a plateau phase was higher in malignant tumors compared to benign lesions (P=.031). Masses with a high MCU showed more frequently a washout of contrast medium after a plateau phase (P=.006) and a higher maximum contrast upslope (P<.001). The MCU delay time was shorter in adenocarcinoma than in squamous cell carcinoma (P=.004). These results indicate that dynamic contrast enhanced MRI might become instrumental in differentiating benign from malignant intrapulmonary tumors and distinguishing adenocarcinoma from squamous cell carcinoma.     

海藻多糖的实验研究——Ⅱ.鼠尾藻和铜藻多糖对EC109细胞株的抑制和对cAMP-PDE的影响

比较了七种海藻多糖在体外抑制人食管癌细胞株(EC109)的生物活性.结果表明,浓度为100毫克/毫升的不同多糖作用48小时后均出现明显抑制,细胞大部分脱落.抑制率在75～95％之间;当浓度为50毫克/毫升作用后抑制率维持在30％以上;25毫克/毫升以下浓度的鼠尾藻多糖仍表现出显著的抑制效果. 各种多糖对组织环化腺苷酸磷酸二酯酶(cAMP-PDE)的体外抑制表明,唯有褐藻淀粉硫酸酯对兔脑cAMP-PDE活性有明显作用,抑制率达74.5％.     

JH11Fab′片段的制备及其在人肝癌裸鼠模型的放射免疫显像

利用胃蛋白酶酶切单克隆抗体ＪＨ１１,经ＳｅｐｈａｄｅｘＧ－１５０分离获得ＪＨ１１Ｆａｂ′,得率约１５％．用氯胺Ｔ碘标法制备１３１Ｉ－ＪＨ１１Ｆａｂ′,观测其在荷人肝细胞癌ＢＥＬ－７４０２裸鼠模型的定位显像作用,腹腔注射１３１Ｉ－ＪＨ１１Ｆａｂ′后２４～１２０ｈ,肿瘤部位放射性物质浓集,并清楚显像,其中尤以９６ｈ为佳．１３１Ｉ－ＪＨ１１Ｆａｂ′注射后９６ｈ,在１３种器官的Ｔ／ＮＴ比值均大于３．０,肿瘤的定位指数为３．８０．这表明ＪＨＦａｂ′片段在肝细胞癌的定位显像和导向治疗中有一个更好的应用前景．     

大肠癌与溃疡性结肠炎患者血清微量元素测定

用ＩＣＰ－ＡＥＳ方法，对３０例大肠癌，２６例溃疡性结肠炎进行血清钼，锌，铬铁，铜，锰，铅，铝，硒，铋，钡，钛，钴，钒等微量元素测定，并与２０例健康者对照。结果显示大肠癌组血清钼，锌，铬，低于对照组钼，锌，铬，Ｐ分别〈０．０１，〈０．０５，〈０．０５；溃疡性结肠炎组钼低于对照组，Ｐ〈０．０５。提示微量元素代谢异常可能直接影响酶系统，使自由基失控，体内促氧化和抗氧化平衡失调，促使肿瘤的发生。     

Expression of integrin β1 and its roles on adhesion between different cell cycle hepatocellular carcinoma cells (SMMC-7721) and human umbilical vein endothelial cells

To investigate expression of integrin β₁ and its roles on adhesion between different cell cycle hepatocellular carcinoma cell (HCC) and human umbilical vein endothelial cells (HUVEC), the synchronous G₁ and S phase HCC were achieved through thymine-2-deoxyriboside and colchicines sequential blockage method and double thymine-2-deoxyriboside blockage method, respectively. Expression of integrin β₁ on hepatocellular carcinoma cells was detected with flow cytometer. Further, the adhesive force of HCC to HUVEC and the role of integrin β₁ in this adhesive course were studied by micropipette aspiration technique. The results showed that percentage of each cyclic phases of the controlled HCC (non-synchronous) are: G₂+M phase, 11%; G₁ phase, 54%; S phase, 36%; the synchronous rates of G₁ and S phase HCC amount to 74 and 98%, respectively. The expressive fluorescent intensity of integrin β₁ in G₁ phase HCC is depressed significantly than the values of S phase and controlled HCC. Accordingly, the adhesive forces of G₁ phase HCC to HUVEC was significantly lower than the value of S phase cells (P<0.01), but it has no remarkable difference when compared the adhesive force values of S phase HCC with control; the contribution of integrin β₁ was about 50% in the adhesion of HCC to HUVEC. It suggested that HCC would be synchronized preferably in G₁ and S phase with thymine-2-deoxyriboside and colchicines, the adhesive molecule integrin β₁ expressed in a high lever in HCC and presented differences in vary cell cycle, and integrin β₁ played an important roles in adhesion of HCC to HUVEC. Possibly, S phase HCC take a great action in this adhesive course.     

100例鼻咽癌患者与健康人头发中微量元素含量的比较

用原子吸收和火焰光谱分析法检测鼻咽癌患者100例，并以本地区100例健康人及同期我院内科住院非肿瘤患者150例为对照组，进行头发锌、铜、铁，钙，镍等的含量进行测定，结果发现鼻咽癌患者头发中钙及镍的含量比对照组明显升高，而头发中锌、铜的含量明显降低。     

Effect of gambogenic acid in attenuating diethylnitrosamine (DEN)-induced hepatocellular carcinoma in rat model

Liver cancer, specifically hepatocellular carcinoma has been a widespread problem among general population. This study aims to investigate the modulating mechanism of gambogenic acid, a phenolic xanthonoid, in diethylnitrosamine (DEN)-induced liver cancer in rats. Male Wistar albino rats were clustered into four groups (n = 6). Group I served as control treated with normal saline. Hepatocellular carcinogenesis was induced in rats by single intraperitoneal (i.p.) administration of DEN in saline (200 mg/kg b.w.) for groups II and III. Group III received oral administration of gambogenic acid (20 mg/kg b.w.) one hour post DEN administration, whereas group IV received oral administration of gambogenic acid (20 mg/kg b.w.) alone. Rats were sacrificed after 16 weeks to determine the levels of hepatic biomarkers, oxidative stress markers, hematological profile and histopathological changes. Gambogenic acid significantly ameliorated the expressions of oxidative stress markers TBARS, GSH (P < 0.05), enzymatic antioxidants GPx, CAT, SOD, GST (P < 0.05), apoptosis mediators (P < 0.05), and serum biomarkers for liver damage and tumor formation (P < 0.05) compared with DEN-induced model group. Hepatocellular levels of 8-OHdG were significantly diminished (P < 0.05) by gambogenic acid against the damage incurred by DEN. Liver histopathological derangements caused by DEN were reversed by gambogenic acid. The results clearly impacted the effect of gambogenic acid in attenuating DEN-induced hepatocellular carcinoma in rats mediated through NF-kβ pathway and hepatocellular oxidative damage.     

紫杉醇、顺铂方案诱导化疗联合放射治疗晚期鼻咽癌近期疗效观察

目的观察紫杉醇(TAX) 顺铂(DDP)方案诱导化疗联合放射治疗晚期鼻咽癌的近期疗效。方法观察组36例鼻咽癌患者给予紫杉醇 顺铂方案化疗,第3周期化疗后1周开始放疗；对照组36例给予单纯放疗。两组放疗方法、剂量相同。结果初治鼻咽癌对TAX DDP方案化疗有较高的反应率,使33.3%病人的T分期和44.4%的N分期降低；近期疗效：观察组鼻咽部及颈淋巴结CR率分别为91.7%及88.9%,较对照组的72.2%和66.7%高(P＜0 05)；化疗及放疗毒副反应两组无明显差别(P＞0.05)。结论TAX DDP方案用于局部晚期鼻咽癌的诱导化疗,具有疗效好、毒副反应轻、患者依从性高等优点,是中晚期鼻咽癌诱导化疗较理想的化疗方案。