Calcium pretreatment increases thermotolerance of Laminaria japonica sporophytes

Calcium is a secondary messenger in plant signaling,and its concentration changes spatially and temporally during the course of heat stress.In the present study,potassium antimonate was used to visualize calcium localization in blades of a marine macroalga,the juvenile Laminariajaponica sporophytes under heat stress (25 ℃).Result showed that loosely bound calcium was mainly distributed on the cell wall under normal conditions (10 ℃),and flowed into the cytoplasm when exposed to heat.The simutaneous assay on the antioxidant system changes was performed.Oxidative damage,as measured by generation of reactive oxygen species (ROS) malondialdehyde (MDA) content,increased significantly during heat stress,and calcium pretreatment alleviated oxidative damage.The assay on the activities of six antioxidant enzymes demonstrated that their enzymatic activities were inhibited when exposed to heat stress,but Ca2+ pretreatment effectively attenuated the inhibition.Results in the present study inferred that calcium homeostasis plays an essential role in L.japonica sporophyte when exposed to heat,and calcium pretreatment could improve its thermo-tolerance.     

金银花U6启动子的克隆及转录活性分析

U6启动子是构建CRISPR/Cas9体系中驱动sgRNA转录的重要元件。利用聚合酶链式反应方法,从华金6号金银花中克隆到3种U6启动子,并分别构建5个不同长度的启动子驱动GUS的植物融合表达载体。以CaMV35S作为阳性对照实验,农杆菌作为阴性对照实验,采用农杆菌瞬时转化法对烟草原生质体进行转染,GUS染色后置于显微镜下观察原生质体的染色数量和效率。根据染色结果,成功筛选出Chr01 U6-F1这一高效转录的启动子,为后期开展金银花的基因编辑奠定了基础。     

柳杉新品种‘福胖’

‘福胖’柳杉是从中国柳杉实生苗中选育出的天然变异新品种。树体矮化，属于灌木，萌蘖能力强，树冠呈窄卵形；雄花期2月底到3月初，雄球花长椭圆形，短作穗状花序，雌花少或无。具有绿化观赏价值，适宜在四川成都平原及盆地的低山丘陵区海拔400～1 200 m作绿化植物栽培，也可以培育为室内观赏植物。     

山葵组培苗瓶外生根技术研究

以山葵试管苗为试材,研究山葵瓶外生根技术。结果表明:用浓度500 mg/L IBA配合松树下腐殖质土,生根率达到75%。该技术可减少组培苗培育环节,缩短山葵育苗生产周期,降低苗木生产成本。