灵芝三萜类化合物高效液相指纹图谱研究

建立灵芝中所含三萜类组分(triterpenoid components,TC)的高效液相指纹图谱.利用Agilent 1100高效液相色谱仪对10批次灵芝TC样品进行了分析.色谱条件为:ZORBAX 300SB C18分析柱(5 μm,250.0 mm×4.6 mm),流动相为甲醇和质量分数为5%的醋酸(体积比4∶6)混合溶液,检测波长252 nm.结果显示,10批次TC样品均出现8个主要特征峰,相对保留时间α分别为0.69,0.78,0.88,0.92,1.00,1.27,1.48和1.61,并且10批次TC样品的8个主峰面积均在70%以上,重叠率均在90%以上.     

Lanostane triterpenoids from cultivated fruiting bodies of the basidiomycete Ganoderma australe

A new lanostane triterpene (1), together with three known compounds (2–4), were isolated from cultivated fruiting bodies of the basidiomycete Ganoderma australe. The structure was elucidated on the basis of NMR spectroscopic and mass spectrometry data. The olefinic geometry of methyl australate (2) was revised from 20(22)Z to 20(22)E. These compounds (1–4) were different from the lanostanes isolated from mycelial cultures of the same strain source.     

松杉灵芝化学成分及药理活性的研究进展

松杉灵芝(Ganoderma lucidum(Curtis)P.Karst)的化学成分包括三萜、多糖、甾体、脂类、氨基酸和微量元素等。松杉灵芝具有广泛的药理学活性,主要包括抗肿瘤、抗高血脂、抗氧化、消炎等作用。本文对松杉灵芝的化学成分、药理作用进行了综述。     

灵芝多糖的研究进展

灵芝多糖是灵芝的主要有效成分之一。主要就近年来灵芝多糖的提取、分离纯化、含量测定、结构分析、硒化修饰和抗氧化、免疫调节、降血糖、抗肿瘤活性等方面的研究进行了综述。     

不同中药提取液对灵芝摇瓶培养的影响

实验采用将9味中药的水提取液浓度0．125g/mL分别加入灵芝二级摇瓶发酵培养基中接种培养,观察中药对灵芝茵液体培养中生长量及产灵芝胞外多糖的影响,并初步研究了四味中药板蓝根、茵陈、垂盆草、茯苓不同浓度对灵芝液体培养的影响。结果显示：茵陈、垂盆草、火巷、桔梗、黄苓、板蓝根、连翘7味中药能抑制胞外多糖的分泌,茯苓可明显地促进灵芝的生长,增强胞外多糖的分泌。茯苓浓度在0．045g／mL-0．165g／mL能增加灵芝生物量,而在浓度0．045g/mL-0．085g/mL时能促进灵芝胞外多糖量,茵陈在浓度0．045g/mL-0．165g／mL范围内时对灵芝的生长具有促进作用,但对胞外多糖存在抑制作用,垂盆草、板蓝根对灵芝胞外多糖量和生物量有一定程度的抑制作用。     

灵芝子实体抗肿瘤成分提取的研究

按表按排正交实验,优化渗漉法提取灵芝中抗肿瘤成分的条件,为灵芝的开发利用提供理论指导,方法乙醇泡后用渗漉法提取有效成分,以渗漉液对ＨｅＬａ细胞的伤害程度作为衡量抗肿瘤成分相对含量的指标。结果灵芝子实体的醇提物在体外具有抑制ＨｅＬａ细胞生长的作用;在影响渗漉条件的４因素中,温度影响最大,渗漉用液量影响最小,结论灵芝子实体中含有抑制ＨｅＬａ细胞生长的成分,以浸泡１ｄ,漉速０．５ｍＬ．ｍｉｎ＾－１,洗脱     

生物转化灵芝功能食品新工艺研究

对灵芝菌生物转化功能食品的生产工艺进行了初步研究,以可食和粮食类培养基代替传统的灵芝培养基,采取先液后固培养灵芝菌新工艺,使经选择的培养基转化成具有灵芝功能的目标保健食品。     

Antitumor Active Protein-containing Glycans from the Body of Ganoderma tsugae

To explore the effects of traditional herbal medicine Ganoderma tsugae(G. tsugae) on immunomodulatory and antitumor activities, the crude polysaccharides of G. tsugae were purified by filtration, diethylaminoethyl(DEAE) sepharose-fast flow chromatography and sephadex G-100 size-exclusion chromatography. Two main fractions, protein- containing glycans CSSLP-1 and CSSLP-2, were obtained via the gradient elution. The protein content, molecular weight, and monosaccharide composition of the two fractions were analyzed. Furthermore, the influence of the protein-containing glycans from G. tsugae on the activation of human acute monocytic leukemia cell line(THP-1) and their antitumor activities to the human hepatocellular liver carcinoma cell(HepG-2) in vitro were evaluated. The results indicate that CSSLP-1 and CSSLP-2 could increase the pinocytic activity of THP-1 cells and induce THP-1 cells to produce the cytokines of TNFα and IL-2, significantly. CSSLP-1 and CSSLP-2 also played an inhibiting effect on the cancer cell(HepG-2). Moreover, the anti-proliferation activity of CSSLP-1 and CSSLP-2 increased with the participation of TNFα and IL-2 or other antitumor factors induced from THP-1 cells by G. tsugae protein-containing glycan fractions.     

大豆粉用于灵芝发酵液制取灵芝醋的研究

将大豆粉作为氮源用于灵芝发酵液的培养,可明显改善发酵液口感利于灵芝醋的生产。试验表明,灵芝发酵液培养基配比为在20%的马铃薯汁中加入葡萄糖2.0%、大豆粉1.5%、KH2PO40.2%、MgSO40.05%,经发酵制取的灵芝发酵液多糖含量可达到0.94 g/L,同时制成的灵芝醋各项指标较好。     

不同部位灵芝及子实体提取物中总三萜酸含量测定

建立不同部位灵芝及子实体提取物中总三萜酸含量测定方法.灵芝样品用乙醇提取,依次经氯仿、5%碳酸氢钠萃取,酸化后再用氯仿萃取、浓缩至干,称重,即得灵芝总三萜酸量.结果:灵芝子实体总三萜酸为(8.58±0.25)mg/g,孢子粉为(3.48±0.03)mg/g,菌丝体仅为(1.75±0.09)mg/g;菌盖总三萜酸含量为(12.62±0.22)mg/g,菌柄为(7.66±0.08)mg/g.10批次灵芝子实体的总三萜酸含量范围为4.3416.39 mg/g.灵芝子实体提取物中的总三萜酸含量以醇-水提取物的最高为(208.70±5.54)mg/g;碱水提取物的最低,仅为(123.07±4.99)mg/g.HPLC初步分析子实体和孢子及子实体提取物间三萜酸成分大部分相同.本法适用于灵芝子实体及其提取物、灵芝孢子和菌丝体总三萜酸的含量测定,结果可靠,可为灵芝产品质量指标提供依据.