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11.
Determination of bioactive compounds in traditional Chinese medicines and biological samples is usually interfered with by coexisting components in matrices. In this work, we prepared novel multilayer functional graphene/polydopamine‐modified polytetrafluoroethylene microtube for selective solid‐phase microextraction of three bioactive compounds in Fructus Psoraleae. Functional graphene/polydopamine‐modified polytetrafluoroethylene microtube showed good extraction efficiency toward bavachin, isobavachalcone, and bavachinin; enrichment from 357‐ to 737‐fold was obtained for these compounds. For qualitative analysis, an online solid‐phase microextraction with high‐performance liquid chromatography method was developed, which showed low limits of detection of 0.02 ng/mL by using UV detection, which is significantly more sensitive than previously reported methods. The proposed method has been used to determine bavachin, isobavachalcone, and bavachinin in Fructus Psoraleae, the contents of three compounds were quantified to be 64.0, 324.0, and 384.5 μg/g; recoveries were 93.4–101.1%. The proposed method has also been applied to determine bavachin, isobavachalcone, and bavachinin in rat plasma samples after oral administration of Fructus Psoraleae.  相似文献   
12.
Ligustri Lucidi Fructus (LLF) is the fruits of Ligustrum lucidum Ait. (Oleaceae). This review based on nearly 80 literary sources discusses the knowledge of chemistry and biological effects of this species. Several types of chemical constituents considered as the characteristic and active constituents from LLF were isolated including 40 triterpenoids, 48 iridoids, 10 flavones, 10 phenylethanoid glycosides and others. Various extracts and individual compounds derived from this species have been found to possess a variety of pharmacological effects, e.g. anti-tumour, hepatoprotective, immune regulating, antioxidative and anti-ageing effects, anti-inflammation and reducing hypercholesterolaemia effects and so on. The results of data analysis on the chemical, pharmacological characteristics of LLF support the view that this species has many therapeutic properties and indicate its potential as an effective herbal remedy. Finally, some suggestions for further research on chemical and pharmacological properties are given in this review. Theoretical basis was given for further exploiting and utilising LLF.  相似文献   
13.
A rapid and sensitive hydrophilic interaction ultra high performance liquid chromatography coupled with triple‐quadrupole linear ion‐trap tandem mass spectrometry method was validated for the simultaneous determination of 20 nucleobases, nucleosides, and nucleotides (within 3.5 min), and then was employed to test the functional food of Luo‐Han‐Guo samples. The analysis showed that the Luo‐Han‐Guo was rich in guanosine and uridine, but contained trace levels of the other target compounds. Chemometrics methods were employed to identify 40 batches of Luo‐Han‐Guo samples from different cultivated forms, regions and varieties. Unsupervised hierarchical cluster analysis and principal component analysis were used to classify Luo‐Han‐Guo samples based on the level of the 20 target compounds, and the supervised learning method of counter propagation artificial neural network was utilized to further separate clusters and validate the established model. As a result, the samples could be clustered into three primary groups, in which correlation with cultivated varieties was observed. The present strategy could be applied to the investigation of other edible plants containing nucleobases, nucleosides, or nucleotides.  相似文献   
14.
Just as natural saponins transform into aglycones, secondary glycosides and their derivatives using biotransformation technology, steroidal saponins may also undergo similar transformation after stir-frying. The purpose of this study was to elucidate the variations and the reasons for these variations in the contents of steroidal saponins in Fructus Tribuli (FT) during a stir-frying treatment. Stir-fried FT was processed in different time–temperature conditions. An UHPLC–MS/MS method was established and fully validated for quantitative analysis. In addition, the simulation processing products of tribuluside A, terrestroside B, terrestrosin K, terrestrosin D and 25R-tribulosin were determined by qualitative analysis using UHPLC–Q-TOF–MS. The established UHPLC–MS/MS method provides a rapid, flexible, and reliable method for the quality assessment of FT. The present study revealed that furostanol saponins with a C22-OH group could transform into corresponding furostanol saponins with a C-20–C-22 double bond (FSDB) via dehydroxylation. Additionally, FSDB could be successively converted into its secondary glycosides via a deglycosylation reaction. The transformation of spirostanol saponins into corresponding aglycones via deglycosylation led to a decrease in spirostanol saponins and an increase in aglycones. The results of this research provided scientific evidence of variation and structural transformation among steroidal saponins. These findings might be helpful for elucidating the processing mechanism of FT.  相似文献   
15.
北五味子的液相色谱指纹图谱的建立   总被引:7,自引:1,他引:7  
付绍平  杨博  陈彤  鱼红闪  金凤燮 《色谱》2008,26(1):64-67
利用反相高效液相色谱法建立中药材北五味子的指纹图谱,为科学评价及有效控制北五味子质量提供了新方法。实验分析了10个不同产地的北五味子样品,采用国家食品药品监督管理局推荐的“中药色谱指纹图谱相似度评价系统(2004 A版)”计算处理,建立了由26个指纹峰和19个共有峰组成的北五味子指纹图谱,确定了5个主要的指纹峰。通过夹角余弦法和相关系数法计算了北五味子10个样本与指纹图谱间的相似度,得到了满意的结果。所建立的指纹图谱可以用来区别不同产地北五味子药材的优劣,为进一步控制北五味子的质量提供依据。  相似文献   
16.
高速逆流色谱分离纯化蔓荆子中的活性成分   总被引:2,自引:0,他引:2  
管仁军  王岱杰  于宗渊  王晓  蓝天凤 《色谱》2010,28(11):1043-1047
应用高速逆流色谱法(HSCCC)分离纯化蔓荆子中的活性成分。以石油醚-乙酸乙酯-甲醇-水(体积比为3:6:3.6:3)为两相溶剂体系,在转速为800 r/min、流速为1.5 mL/min、检测波长为254 nm的条件下进行分离,所得馏分经高效液相色谱法(HPLC)检测,并经电喷雾电离(ESI)质谱和核磁共振谱(NMR)鉴定化合物的结构。从250 mg蔓荆子粗提物中一次性分离得到4个化合物,分别为23 mg对羟基苯甲酸、15 mg 3,6,7-三甲基槲皮万寿菊素、24 mg蔓荆子黄素和5 mg蒿黄素,其纯度约为93.1%、 97.3%、 98.7%和98.5%。该法具有简便、快速、重复性好的优点,为分离蔓荆子中的活性成分提供了新的方法。  相似文献   
17.
构建了一种新的微乳体系,用于微乳液相色潜同时分析吴茱萸药材中的辛弗林、吴茱萸碱和吴茱萸次碱.用甲醇超声提取方法制备样品,通过对表面活性剂种类及浓度、酸度、添加剂等影响因素进行考察,得到最佳微乳体系的组成为:3.0%SDS-6.0%正丁醇-0.6%正辛烷-1.0%甲酸-1.2%乙腈-88.2%水.选择Diamonsil ...  相似文献   
18.
A simple, sensitive and selective method of high-performance liquid chromatography with electrochemical detection (HPLC-ECD) has been developed for simultaneous determination of bavachin and isobavachalcone in Fructus Psoraleae. At optimized conditions, bavachin and isobavachalcone could be well separated within 15 min at a detection potential of +0.80 V with 0.03 mol/L acetate buffer solution (pH 5.17)/acetonitrile (2:3, v/v) as the mobile phase. The relationships between peak areas and concentrations were linear from 8.26 × 10(-7) to 1.21 × 10(-4) mol/L for bavachin, and from 1.01 × 10(-8) to 1.61 × 10(-4) mol/L for isobavachalcone, respectively. The method offered excellent linearity with regression coefficient R(2) >0.995. The method presented detection limits (S/N = 3) of 8.81 × 10(-9) mol/L for bavachin and 1.17 × 10(-10) mol/L for isobavachalcone. It indicates that the sensitivity of electrochemical detection is ten times higher than that of diode array detection (DAD). The mean recoveries around 98% with a relative standard deviation less than 3.1% for the two analytes have been obtained. The proposed separation and detection procedures were successfully applied to the simultaneous determination of bavachin and isobavachalcone in traditional Chinese medicine.  相似文献   
19.
Fructus cnidii (Chinese name shechuangzi) is the fruit produced by Cnidium monnieri (L.) Cusson (Umbelliferae). It is a perennial herb that is used to treat skin-related diseases and gynecopathyell. Recent pharmacological studies have revealed crude extracts or components isolated from fructus cnidii possess antiallergic, antipruritic, antidermatophytic, antibacterial, antifungal, and antiosteoporotic activities. Osthole and imperatorin are the major compounds present in shechuangzi. They are often used as standards for the evaluation of the quality of shechuangzi products.  相似文献   
20.
微波辅助提取-GC/MS联用分析苍耳子中油脂成分的研究   总被引:2,自引:0,他引:2  
采用微波辅助提取-GC/MS联用分析苍耳子中的油脂成分.以V(环已烷):V(丙酮)=1:1为提取溶剂,优化了微波辅助提取的条件.以正二十烷为内标,鉴定出了13种油脂成分,各组分相对保留时间的相对标准偏差(RSD)小于0.15%,相对峰面积的RSD<3.0%.对不同批次以及不同产地的苍耳子样品进行了对比研究.该方法可用于苍耳子的鉴定和质量控制.  相似文献   
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