采用正交设计和响应曲面设计法优化芦苇随机扩增多态DNA体系

采用正交设计和响应曲面设计(RSD)优化适合于芦苇[Phragmites australis (Cav.)Trin.ex steud]增多态DNA(RAPD)的反应体系,结果确定芦苇RAPD反应体系的最佳方案为：在PCR扩增程序,冷启动,94℃变性1min,36℃复性90s,72℃延伸2min,40个循环;72℃延伸7min,4℃保存;25μl反应体系包括模板DNA 60ng、三磷酸脱氧核苷酸(dNTP)7．5mmol、Taq酶1．5u、引物5pmol。     

基于AT89S52的数字温度计设计

以DJ-598K实验系统为开发平台,以AT89S52为主控芯片设计数字温度计,重点介绍基于DS18B20的温度采集模块和基于SG12864的LCD显示模块的硬件设计和软件实现.     

基于PID算法的水温控制系统的设计

为了实现水温控制系统的设计要求,通过对各个模块电路方案的比较和论证,最后确定了以STC89C52单片机为核心的硬件电路,选用DS18B20温度传感器测量水温。系统可以实时地显示温度,并能通过温度设置来对水的温度进行调节,同时具有报警功能。另外,串口通讯程序的设计使得可以同步地在电脑上显示出水温的曲线。核心的控制算法基于PID控制。该设计实例也适用于高校单片机及相关课程的教学实践活动。     

基于LSSVM概率输出与证据理论融合的变压器故障诊断

为了利用相对较少的故障数据样本对变压器主要故障类型进行较准确的判断,基于智能互补和数据融合的思想,提出基于最小二乘支持向量机LSSVM( least square support vector machine)概率输出与证据理论融合的故障诊断方法。该诊断方法具有以下特点：可融合蕴含变压器运行状态的多种特征信息,输出变压器各种故障的概率,为变压器检修提供更多的可用信息;充分发挥了LSSVM在小样本情况下具有较强泛化能力的优势。算例结果表明,该诊断方法的故障诊断准确率达到91.1%,优于传统的IEC三比值法(故障诊断准确率75.6%)及LSSVM分类法(故障诊断准确率82.2%),有效降低了诊断误判的风险。     

Development of an enzyme-linked immunosorbent assay (ELISA)-like fluorescence assay to investigate the interactions of glycosaminoglycans to cells

Sulfated glycosaminoglycans were labeled with biotin to study their interaction with cells in culture. Thus, heparin, heparan sulfate, chondroitin 4-sulfate, chondroitin 6-sulfate and dermatan sulfate were labeled using biotin-hydrazide, under different conditions. The structural characteristics of the biotinylated products were determined by chemical (molar ratios of hexosamine, uronic acid, sulfate and biotin) and enzymatic methods (susceptibility to degradation by chondroitinases and heparitinases). The binding of biotinylated glycosaminoglycans was investigated both in endothelial and smooth muscle cells in culture, using a novel time resolved fluorometric method based on interaction of europium-labeled streptavidin with the biotin covalently linked to the compounds. The interactions of glycosaminoglycans were saturable and number of binding sites could be obtained for each individual compound. The apparent dissociation constant varied among the different glycosaminoglycans and between the two cell lines. The interactions of the biotinylated glycosaminoglycans with the cells were also evaluated using confocal microscopy. We propose a convenient and reliable method for the preparation of biotinylated glycosaminoglycans, as well as a sensitive non-competitive fluorescence-based assay for studies of the interactions and binding of these compounds to cells in culture.     

基于LabVIEW与单片机的温度控制系统设计

温度已经成为生产、科研的一项重要环境参数,对于该参数的采集与控制已经显得非常重要。本系统利用LabVIEW软件进行PC上位机设计,采用串口通信的方式实现与单片机进行温度数据传输及指令控制,温度数据采集用集成温度传感器DS18B20实现。该系统硬件电路设计简单,功能完善,易于修改且扩展性强。     

Statistical solution to the capacity problem in direct-sequence code-division multiple-access communication systems

Tohru Kohda Department of Computer Science and Communication Engineering, Kyushu University, Fukuoka 812-8581, Japan Minoru Tabata Department of Mathematical Sciences, Osaka Prefecture University, Osaka 599-8531, Japan Email: eshima{at}med.oita-u.ac.jp Received on September 21, 2005; Accepted on May 2, 2006 Capacity in the direct-sequence code-division multiple-access(DS/CDMA) communication system was considered according to thecode acquisition performance with the conventional serial-searchmethod, because code acquisition needs a difficult operation.Since capacity in DS/CDMA systems is defined by the maximumnumber of users that can simultaneously transmit their signalswith the same carrier frequencies, assuring a larger capacityis very important in DS/CDMA systems with respect to the economyof frequency source. This paper reconsiders the capacity problemthrough a statistical approach to code acquisition. First, aDS/CDMA system model is reviewed. Second, properties of thecounting method for code acquisition are discussed. It is provedthat the method can acquire the target user's code under anynumber of interference users that simultaneously transmit theirsignals, and that the method can guarantee a considerable precisionin code acquisition. Third, an observation time necessary forcode acquisition is given from a statistical discussion, andthe original counting method is modified into a simpler one.It is concluded that the capacity of the DS/CDMA communicationsystem can be set by the ‘bit error-rate-based capacityor signal-to-noise ratio-based capacity’, i.e. ‘post-acquisition-basedcriterion’, rather than the ‘acquisition-based capacity’.     

结合可信度和DS证据理论的ECOC多类分类研究

针对多类目标识别问题,利用三符号纠错输出编码作为将多类分解为若干个二类问题的结构框架,用改进的证据理论作为融合策略,将每一个二分器的输出作为证据之一进行融合;同时对分类器的可信度进行估计,并将得到的信任度量值融入证据中,从而提高多类分类的正确率。实验中分别对UCI数据集和三种一维距离像数据集进行测试,结果表明本文提出的基于分类器可信度的多类目标识别方法能有效地提高复杂环境下多传感器目标识别的正确率。     

利用UG和3DS MAX的结合实现冲模三维虚拟动态装配

利用UG建立了冲模装配体及其零件的三维实体模型,结合3DSMAX,从后往前制作拟实动画,把冲模装配的过程进行了三维动态处理．实现了冲模三维虚拟动态装配,利用这一方法能保证冲模设计的准确性,同时可指导装配轻松地完成冲模组装．     

基于DS18B20模块固化的CRC校验漏检率补偿算法研究

为了提高DS18B20温度数据的可靠性,通过降低DS18B20模块自身CRC校验的漏检率,提出了一种温度采集补偿算法。该算法对DS18B20模块采集到的温度数据进行分析,排除数据队列中相对错误率较高的数据,再经滤波和迟滞处理后作为实际温度数据。结果表明,在给定的精度下,选定相应的数据队列长度及排除数据数,该算法不仅能够降低DS18B20的CRC漏检率,同时能平滑温度显示数据,有效抑制温度显示的闪烁现象。