Biparametric multicommutated flow analysis system for determination of human serum phosphoesterase activity

A multicommutated flow analysis (MCFA) system constructed of microsolenoid valves and pumps offering simultaneous determination of activity of acid phosphatase (ACP) and alkaline phosphatase (ALP) in human serum samples has been developed. The MCFA system is based on optoelectronic flow-through detector made of two light emitting diodes and operating according to paired emitter detector diode (PEDD) principle. This photometric PEDD device has been dedicated for detection of p-nitrophenol (NP) generated in the course of enzymatic hydrolysis of p-nitrophenyl phosphate and optimized for the determination of NP in human serum samples. The developed PEDD-based MCFA system allows independent optimization of conditions for reaction and detection steps of photometric ACP and ALP bioassays. Moreover, it allows elimination of photometric interferences from serum matrix components according to two-points kinetic mode of measurement. The single measurement cycle takes 12 min, consists of four measurements (two for each phosphoesterase) and enables determination of serum ACP and ALP activities at physiological and pathological levels. The real analytical utility of the developed MCFA system has been confirmed by analysis of control sera as well as real human serum samples from healthy persons and oncological patients.     

情景教学模式在妇产科护理带教中的应用

目的：探讨情景教学对妇科带教的意义；方法：设立实验组和对照组进行实习结果的对比，对照组采用传统教学，实验组采用情景教学；结果：实验组与对照组在无菌观念、配合熟练度、工作积极性、沟通能力、代教满意度等方面，对比都有显著差异（P<0.001）；结论：情景教学对妇科教学意义重大，能够有效提高教学质量.     

Advances in nucleic acid architectures for electrochemical sensing

Nucleic acid–based electrochemical sensors are ideally suited to the detection of molecular targets for which enzymatic detection or direct electrochemical oxidation – reduction reactions are not possible. Moreover, the versatility of nucleic acids in their ability to bind a great variety of target types, from small molecules to single-entity mesoscopic targets, makes them attractive receptors for the development of electrochemical biosensors. In this brief opinion piece, we discuss field advances from the past two years. We hope the works highlighted here will inspire the community to pursue creative designs enabling the detection of larger and more complex targets with a specific focus on analytical validation and translation into preclinical or clinical applications.     

临床医学专业有机化学"课程思政"教学初探

深化高校"课程思政"改革,是落实立德树人理念,全方位对学生开展思想政治教育的有效途径。本文针对临床医学专业的特点,从教师素养的提高、课程思政内容的设计和学生的课外实践等方面对有机化学教学中实施"课程思政"进行了探索和实践。     

A statistical dynamics approach to the study of human health data: Resolving population scale diurnal variation in laboratory data

Statistical physics and information theory is applied to the clinical chemistry measurements present in a patient database containing 2.5 million patients' data over a 20-year period. Despite the seemingly naive approach of aggregating all patients over all times (with respect to particular clinical chemistry measurements), both a diurnal signal in the decay of the time-delayed mutual information and the presence of two sub-populations with differing health are detected. This provides a proof in principle that the highly fragmented data in electronic health records has potential for being useful in defining disease and human phenotypes.     

五年制临床医学实践教学存在的问题及对策探讨

该文通过文献研究和实地考察，结合部分学生的个别访谈，从临床实践教学课程的设置、方法的改进、实践基地的机制建设、教学评价这四个方面有针对性地分析了我国五年制临床实践教学中存在的主要问题及其根源，运用行为主义的强化学习理论，以临床实践运用操作技能为核心，整合有限的教学资源，兼顾学生的个性发展，在遵循学生认知规律的基础上，提出四个方面科学、合理的对策以期为丰富我国五年制临床实践教学的研究提供相关的参考依据。     

探讨宫内节育器在人工流产术后即刻放置的临床疗效

目的:探讨宫内节育器在人工流产术后即刻放置的临床疗效。方法:将自愿要求人工流产终止妊娠短期内无生育要求的健康育龄妇女600例随机分为观察组和对照组各300例。观察组为人工流产术后即刻放置宫内节育器,对照组不予特殊处理。观察两组术后阴道流血量、出血时间、月经复潮时间、痛经、闭经、避孕效果的情况及不良反应。结果:观察组人工流产术后阴道流血量、流血时间、月经复潮时间、痛经、闭经与对照组无明显差异,避孕效果优于对照组(P﹤0.05),无体重增加,无不良反应。结论:人工流产术后即刻放置宫内节育器可有效减少重复流产及宫腔、宫颈粘连、盆腔感染,是一种安全、有效并简单的避孕方法,值得推广。     

困难子宫摘除307例临床分析

目的：探讨困难子宫除的方法和手术原则。方法：对大理州妇幼保健院1982年至2001年困难子宫除307例进行临床分析。结果：下列为困难子宫除情况，即：宫颈肌瘤78例，巨大子宫肌瘤74例，严重粘连的子宫肌瘤67例，阔韧带肌瘤65例，侵蚀性葡萄胎15例，子宫肌瘤并妊娠8例。该组病例手术成功，末发生意外损伤。手术严重出血的病例经及时抢救康复。结论：术前诊断明确，术中处理方法得当，操作认真仔细是做好困难子宫除的三大要素。     

激素治疗急性脊髓损伤的研究进展

综述了激素治疗急性脊髓损伤方面的研究进展 ,内容涉及到其基础研究和临床应用。     

红外光弹三向进光法研究硅晶片的应力状态

本文根据双折射原理,采用红外光弹三向进光法,对硅晶片的二维应力状态进行测量和研究。测定了在器件加工中{111}和{100}硅晶片各层的主应力的大小和方向,并对测量误差、三个进光方向选定和测量结果进行了分析和讨论。