花粉管通道法转基因技术在甜瓜品种河套蜜瓜上的应用

为建立甜瓜(Cucumis melo L.)简便易行转化频率高的转基因技术,以甜瓜品种河套蜜瓜为受体材料,以含gus基因的植物双元表达载体pPZP221为外源基因供体,进行了花粉管通道法转基因研究.自交授粉后分别于1、2、3、4、5、6、7、8、9、10 h,切去柱头上端1/3部分,立即滴加质粒DNA溶液,果实成熟后收获转化种子.对T0代植株进行PCR检测,结果表明不同时间处理所得到的T0代植株均具有较高的转化频率,其中授粉后7 h滴加DNA溶液所获得的转化率为28.3%.对一部分PCR阳性的T0代植株基因组DNA进行Southern杂交分析,结果表明所有样品均出现特异性杂交条带,证明外源基因已整合到受体植物基因组中.     

Effect of Biodegradable Coatings on the Growth of Aspergillus flavus In Vitro,on Maize Grains,and on the Quality of Tortillas during Storage

The fungus Aspergillus flavus causes serious damage to maize grains and its by-products, such as tortilla. Currently, animal and plant derivatives, such as chitosan and propolis, and plant extract residues, respectively, are employed as alternatives of synthetic fungicides. The objective of this research was to evaluate the efficacy of several formulations based on propolis-chitosan-pine resin extract on the in vitro growth of A. flavus, the growth of maize grain plantlets and the quality of stored tortillas at 4 and 28 °C. The most outstanding formulation was that based on 59.7% chitosan + 20% propolis nanoparticles + 20% pine resin extract nanoparticles; since the in vitro conidia germination of A. flavus did not occur, disease incidence on grains was 25–30% and in tortillas, 0% infection was recorded, along with low aflatoxin production (1.0 ppb). The grain germination and seedling growth were markedly reduced by the nanocoating application. The percentage weight loss and color of tortillas were more affected by this coating compared to the control, and the rollability fell within the scale of non-ruptured at 4 °C and partially ruptured at 28 °C. The next step is to evaluate the toxicity of this formulation.     

关于L-fuzzy子环的L-fuzzy同态的新刻画

给出L-fuzzy子环的L-fuzzy同态和L-fuzzy子环的L-fuzzy模的L-fuzzy同构的一种新刻画.     

刺槐(Robinia pseudoacacia)无性系种群结构与生长动态的研究

研究了刺槐克隆种群的种群结构和生长动态.结果表明,刺槐克隆种群中幼年分株数量较多,死亡率较高;成年个体数量较少,死亡率较低,而且相对稳定,种群数量结构成金字塔型;无性系产生分株的时间较晚,分株的数量正处于上升阶段.无性系中刺槐基株的基径、高度、冠幅远远大于分株.刺槐分株在幼年时期高生长和冠幅的生长均较快,当生长到一定的年龄逐渐缓慢下来.克隆分株的生长、数量动态主要受克隆整合的内部调节机制和外部环境中灌木层、草本层竞争的影响.     

苦瓜的营养成分研究

用自动定氮仪、氨基酸自动分析仪分析测定苦瓜 (Momordica charantia L .) 5个品种 (当地栽培苦瓜、长白苦瓜、长白 1号苦瓜、 89- 1苦瓜、 92 - 2苦瓜 )的干物质含量、维生维 C含量、可溶性糖含量、蛋白质和氨基酸、矿物质含量及其种子的蛋白质含量。结果表明 ,苦瓜果实含干物质 6 %～ 7% ,品种间的差异不显著 (P>0 .0 5 ) ;每 10 0 g嫩苦瓜中含维生素 C6 0～ 80 mg,可溶性糖 1.0 8～ 1.5 5 mg,品种间的差异都不显著 (P >0 .0 5 ) ;苦瓜果实的蛋白质含量为 1.3%～ 1.8%、总氨基酸含量为 1.3%～ 1.5 % ,种子蛋白质含量高达 39%～4 5 % ,各种成分相对含量有显著性差异 (P <0 .0 1) ,不同品种之间存在蛋白质多态性变化。苦瓜种子的营养价值很高。     

辐射诱变愈伤组织选育耐寒型蕨菜

用γ射线对具有100%分化率的蕨菜愈伤组织进行辐射处理,将未致死的愈伤组织于1~2℃无光条件下处理20 d,再把未冻死的愈伤组织所分化的试管苗进行低温处理.将低温处理后的试管苗叶片、叶柄诱导的愈伤组织和分化的试管苗再进行低温选择,获得了耐寒蕨菜试管苗,由这种试管苗所形成的孢子苗仍然具有耐寒性.结果证明用剂量为5.16×10-1C.kg-1的γ射线对蕨菜的愈伤组织进行辐射处理,可选育出耐寒型蕨菜.     

构树生长特性研究

本文采集贵阳市花溪区3株构树,通过树干解析方法,研究其树木生长特性。结果表明:构树生长快,11年生时构树单株平均胸径总生长量已达17.35 cm、胸径连年生长量大,一般1.5 cm左右,树高总生长量13.43 m、材积总生长量0.1572m3,胸径速生期在5-7 a。     

网格图的多重2-分离L(2,1)-标号

研究了两种网格图;正三角形,正六边形网格图。研究了它们的n重2-分离L(2,1)-标号以及n重2-分离L(2,1)-圆标号。用Kn表示n个点的完全图,图G的n重2-分离L(2,1)-标号就是复合图G[Kn]的L(2,1)-标号。通过对两种网格图的顶点循环地分配标号集,得到了正三角形网格的n重2-分离L(2,1)-标号数取值范围,并且完全确定了正六边形网格的n重2-分离L(2,1)-标号数。     

壮药岗松油的研究概况

岗松油为桃金娘科植物岗松（Baeckea frutescens L.）的枝叶经水蒸气蒸馏提取的挥发油,其主要化学成分为蒎烯、1-8桉叶素、芳樟醇、石竹烯、律草烯等化合物。采用薄层色谱法和气相色谱法作为岗松油药材的质量控制方法,芳樟醇和桉油精为其质量监控的重要指标之一。岗松油对化学性肝损伤有明显的预防作用;对巴豆油引起的小鼠耳部炎症有明显的抗炎作用;对小鼠神经系统有一定的抑制作用;对麻醉猫的呼吸系统、心血管系统无明显的影响。岗松油主要用于外用抗菌消炎、保洁护肤、洗液等,还可以应用于香料工业和轻化工等领域。岗松油应用前景广阔,应对其进行系统深入研究开发,使其资源优势转化为产业优势。     

Expression of lysine-rich protein gene and analysis of lysine content in transgenic wheat

Expression vector pBPC102, which carries winged bean lysine-rich protein (wblrp) gene and dihydropicolinate synthase (DHDPS) gene, was transferred into hexaploid winter wheat cv. Jinghua No.l, Jing411, You899 and Yangnongl5 explants of immature inflorescence and immature embryos by particle bombardment. More than 100 transgenic plants were obtained under the selection of s-(2-aminoethyl)-L-cysteine (AEC). Confirmed transgenic plants of To and TI generation by PCR and PCR-Southern blotting analyses showed successful integration of wblrp gene into wheat genome. Analysis of transgenic plant lines of T2 by Northern dot-blotting showed good expression of wblrp gene in offspring seed. The content of free lysine in leaves, contents of bound lysine and total proteins in seeds of T2 transgenie wheat lines were determined and analyzed. Among 34 tested transgenic lines, levels of free lysine content in leaves of 9 transgenic lines are 2~3times higher than un-trans-formed wild-type cultivars. Among 17 analyzed transgenic lines, bound lysine content of 4 transgenic lines is more than 10% higher than that of wild-type cultivars. Our research suggests that introducing wblrp gene into wheat is an effective way to improve its nutrition quality.