3-[(4'-吗啉基)-羰基或乙氧羰基]-4-苯氨基喹啉化合物的合成及抗肿瘤活性

以间氯苯胺与乙氧基亚甲基丙二酸二乙酯(EMME)为原料, 经缩合、 高温环合、 水解、 氯代和亲核取代等反应设计并合成了16个3位为(4'-吗啉)-羰基或乙氧羰基的4-苯氨基喹啉化合物(Ⅰ₁~Ⅰ₁₀, Ⅱ₁~Ⅱ₆). 目标化合物结构经MS及¹H NMR确证. 以MTT法, 采用表皮生长因子受体(EGFRs)高表达的人癌细胞(HeLa, HepG2, BGC-823)对目标化合物进行活性测试, 结果表明, 该类化合物对HeLa, HepG2和BGC-823细胞增殖具有一定程度的抑制作用. 其中化合物Ⅱ₄~Ⅱ₆ 对 HepG2 细胞抑制作用较强, 化合物Ⅱ₁和Ⅱ₅对BGC-823细胞抑制作用较强. 初步探讨了化合物结构与生物活性的关系.     

可高亲和结合两种不同抗原的抗体

抗体和抗原的相互作用面常常被描述成锁和钥匙的关系,说明一个抗体只能适合一种抗原。美国加州基因技术公司Germaine Fuh和同事分离出一种治疗用单克隆抗体Herceptin的异构体,其结合位点可以同时结合人表皮生长因子受体2（HER2）和血管内皮生长因子（VEGF）     

三种花灌木叶表皮特征扫描电镜观察

利用扫描电镜观察了燕山东段三种花灌木木半夏（Elaeagnus multiflora Thunb．）、达乌里胡枝子（Lespedeza daurica （Laxm．）Sehindl．）和蚂蚱腿子（Myripnois dioica Bge．）的叶表皮特征。结果表明：①蚂蚱腿子的上下表皮均无表皮毛分布;木半夏上下表皮都有表皮毛分布;达乌里胡枝子的表皮毛仅分布在下表皮。②达乌里胡枝子的上下表皮都有气孔分布;木半夏、蚂蚱腿子仅下表皮有气孔分布。③达乌里胡枝子的气孔下陷;木半夏、蚂蚱腿子的气孔不下陷。④木半夏、达乌里胡枝子、蚂蚱腿子的上下表皮细胞表面平缓。     

考虑井储和表皮的复合油藏干扰试井分析

目前,国内外的干扰试井理论已有了相当程度的发展,均质油气藏的干扰试井理论及分析方法已比较完善,双重介质油气藏干扰试井理论相对也比较完整。但是,有关复合油气藏试井的试井理论和分析方法还很少,尤其是考虑井储和表皮的复合油气藏试井理论更少。因此,提出了考虑井筒储集和表皮系数影响的复合油藏试井模型,并利用拉氏变换进行了求解,制作了相应的样版曲线。     

Promoter of soybean early nodulin gene<Emphasis Type="Italic">enod2B</Emphasis> is induced by rhizobial Nod factors in transgenic rice

Nod factors, which are signaling molecules produced by Rhizobia, are the principal determinants of host specificity in Rhizobium-legume symbiosis. Nod factors can elicit a number of characteristic developmental responses in the roots of legumes, such as depolarization of the membrane potential in epidermal cells, specific expression of early nodulin genes and changes in the flux of calcium in root hairs, deformation of root hairs, cell division in the root cortex and formation of the nodule primordinm. Whether the rice plant can respond to signaling molecules (i.e. Nod factors) is an important question, as it could establish the potential for symbiotic nitrogen fixation in rice. The promoter of the soybean (Glycine max) early nodulin gene Gmenod2B fused to the β-glucuronidase (GUS) reporter gene was used as a molecular marker to explore whether Nod factors can be recognized by rice cells as signaling molecules. Transgenic rice plants harboring the chimeric gene Gmenod2BP-GUS were obtained via an Agrobacterium tumefaciens-mediated system. NodNGR factors produced by a broad-host-range Rhizobium strain NGR234(pA28) were used as probes to investigate the activity of the Gmenod2B promoter in rice. Our results showed that the early nodulin gene Gmenod2B promoter was induced by NodNGR factors in transgenic rice, and that it was specifically expressed in rice plant roots. Moreover, GUS gene expression driven by the Gmenod2B promoter in transgenic rice was regulated by nitrogen status. These findings indicated that rice possessed the ability to respond to Nod factor signals, and that this signal transduction system resulted in activation of the Gmenod2B promoter. Thus, we predict that the Nod-factor inducible nodulin expression system, which is similar to Rhizobium-legume symbiosis, may also exist in rice.     

消化性溃疡根除幽门螺杆菌前后血中前列腺素E_2和表皮生长因子变化的临床研究

本文重点研究消化性溃疡(PU)中幽门螺杆菌(HP)与前列腺素E_2(PGE_2)和表皮生长因子(EGF)之间的关系。胃镜证实良性消化性溃疡39例,HP均阳性,男26例,女13例,平均年龄41.2岁,接受德诺、弗莱莫新、灭滴灵“三联”治疗,停药4周后复查胃镜了解PU和HP治疗情况,并在治疗前和复查后分别给患者抽血2ml用放免方法检测PGE_2和EGF的变化情况。结果HP根除前PGE_2高于正常组,EGF低于正常组,HP根除后,PGE_2和EGF均接近正常,说明HP的存在不仅起着溃疡病的致病作用,而且影响保护胃肠粘膜的胃肠激素水平,同时可将PGE_2和EGF作为判断根除HP疗效的指标之一。     

蜕皮周期中荒漠沙蜥表皮的组织学变化

应用半薄切片技术，对荒漠沙蜥表皮在蜕皮周期中的组织学变化进行了观察，荒漠沙蜥的表皮在一个完整的蜕皮周期可分为６个发育时期，其中，在时期Ⅰ即休止期中，存在一个特殊的发育时相２，即中层胀厚期，因此，休止期可细分为４个发育时相，此外，冰冻切片，苏凡Ⅲ染色，仅中层染为鲜艳橙色，显示中层不但存在，而且是富含脂数物质的结构，因此，中层可能对荒漠沙蜥皮肤防止水分蒸发的渗透屏障的建立起重要作用。