A New Application of Carbon Nanotubes Constructing Biosensor

Carbon nanotubes used for constructing biosensor was described for the first time. Single-wall carbon nanotubes (SWNTs) functionalized with carboxylic acid groups were used to immobilize glucose oxidase forming a glucose biosensor. The biosensor response can be determined by amperometric method at a low applied potential (0.40V).     

层状嵌接式钴酞菁／NaCIO体系环氧化催化研究

层状嵌接式钴酞菁／ＮａＣＩＯ体系环氧化催化研究胡希明皮宗新谷云骊黄仲涛＊（华南理工大学应用化学系＊化学工程系广州５１０６４１）生物体系的叶绿素和血红蛋白将配位活性中心包覆于蛋白质肽链所构成的空间立体环境中，近年来利用无机材料的刚性，将金属酞菁固载于分...     

醇脱氢酶电极的研制

通过化学交联法将醇脱氢酶(ADH)固定在玻碳电极表面(Φ=5mm),使用N-甲基吩嗪甲基疏酸盐(PMS)和铁氰化钾为介体、间接测定酶促反应中生成的还原辅酶Ⅰ(NADH)。工作电位+300mv(vs.SCE)乙醇测定的线性范围为0.05～1.0mmol/L,响应时间小于20s。如果电极每天测定30次,可以使用两周。并对电极的选择性进行了讨论。     

长链烷氧取代金属卟啉的合成及催化性能研究

本文报道了一类长链烷氧取代金属卟啉化合物3的合成.研究了由化合物3与表面活性剂:十二烷基三甲基溴化铵所形成的混合胶束体系作为仿细胞色素P-450加氧酶模拟物对苯乙烯环氧化的催化活性.结果表明:在胶束体系中长链烷氧取代金属卟啉的催化效率优于对称四甲苯基金属卟啉,由此证实:混合胶束中,疏水内核提供的模拟酶的疏水微环境,在苯乙烯环氧化反应中起着重要作用.     

催化反应热诱导激光光偏转分析法测定尿素

报道用催化反应热诱导激光光偏转法测定痕量尿素。发现将脲酶制成一定浓度的乙醇浓液能显著地增强激光光偏转信号强度。探讨了增强作用机理以及光偏转号强度与尿素浓度的关系，并应用于尿样中尿素含量的测定，结果满意。     

聚邻苯二胺修饰电极抗坏血酸氧化酶生物传感器的研究

本文报道了聚邻苯二胺／抗坏血酸氧化酶生物传感器，用这种传感器测定人体血清中的抗坏血酸，线性范围在１．０×１０＾－４～２．５×１０＾－７ｍｏｌ／Ｌ之间，响应时间为７ｓ，检测限为１．０×１０＾－８ｍｏｌ／Ｌ。该传感器具有选择性好、灵敏度高和响应时间短等特点。     

A New Amperometric Biosensor Based on HRP/Nano-Au/L-Cysteine/Poly（o-Aminobenzoic acid）-Membrane-Modified Platinum Electrode for the Determination of Hydrogen Peroxide

The third generation amperometric biosensor for the determination of hydrogen peroxide （H2O2） has been described. For the fabrication of biosensor, o-aminobenzoic acid （oABA） was first electropolymerized on the surface of platinum （Pt） electrode as an electrostatic repulsion layer to reject interferences. Horseradish peroxidase （HRP） absorbed by nano-scaled particulate gold （nano-Au） was immobilized on the electrode modified with polymerized o-aminobenzoic acid （poABA） with L-cysteine as a linker to prepare a biosensor for the detection of H2O2. Amperometric detection of H2O2 was realized at a potential of ＋20 mV versus SCE. The resulting biosensor exhibited fast response, excellent reproducibility and sensibility, expanded linear range and low interferences. Temperature and pH dependence and stability of the sensor were investigated. The optimal sensor gave a linear response in the range of 2.99×10^-6 to 3.55×10^-3 mol·L^-1 to H2O2 with a sensibility of 0.0177 A·L^-1·mol^-1 and a detection limit （S/N = 3） of 4.3×10^-7 mol·L^-1. The biosensor demonstrated a 95% response within less than 10 s.     

镀金和碳纳米管修饰金电极上吸附态葡萄糖氧化酶比活性的EQCM研究

采用石英晶体微天平(EQCM)技术监测了裸金电极、镀金和碳纳米管修饰金电极上葡萄糖氧化酶(GOD)的吸附过程. 通过EQCM测量吸附固定的GOD质量, 并实时检测酶反应产物H2O2的氧化电量, 求算了各表面上吸附态GOD的比活性(ESAi). 结果表明, 各表面上均可吸附一定的GOD, 且吸附态GOD均有一定的酶活性; 修饰CNTs可增大酶吸附量和酶电极对葡萄糖的响应电流, 但ESAi随CNTs修饰量的增大而降低; Au电极上电镀金后, 酶吸附量和酶电极对葡萄糖的响应电流亦增大, 但ESAi与裸金电极上的基本一致.     

离子液体中的生物催化反应

本文综述了近年来离子液体中生物催化反应的研究进展。离子液体作为新的绿色溶剂,用于生物催化反应具有以下特点:在离子液体中酶有良好的稳定性、选择性和反应活性。离子液体可溶解极性大的反应物, 产物易分离,酶和离子液体可重复使用。对离子液体中的生物催化进行了展望。     

Preparation and Properties of Urease Immobilized onto Glutaraldehyde Cross-linked Chitosan Beads

Urease was immobilized onto the glutaraldehyde cross-linked chitosan beads that were prepared under microwave irradiation. The activity and the yield of activity of immobilized urease was 10.83 U/g B and 47.7%, respectively. The conditions of urease immobilization were optimized. The properties of the immobilized urease were investigated and compared with that of the free enzyme.