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1.
在1L搅拌发酵罐中对含有重组质粒pRLK14的大肠杆菌进行了培养。结果表明,采用二段培养法,于34℃增殖细菌,在对数增殖后期升温到42℃进行诱导,可以高效表达基因产品半乳糖激酶。诱导期,醋酸浓度增长较快,模拟实验表明,控制醋酸浓度可进一步提高半乳糖缴酶产率。 相似文献
2.
By using optical microstructure(OM), X-ray diffractometer analyses and mechanical properties testing, the abnormal damage of pipe coil in remaining heat boiler has been investigated. The damage is the result of the melting of tube base metal at high temperature caused by the ignition inside the tube. 相似文献
3.
本文报道了一种测角单晶NMR探头。该探头采用单线圃双调谐电路,工作频率在90MHz-110 MHz连续可调,可进行交叉极化大功率去耦实验。文中提出了一种简单而有效的测角装置,可使单晶绕三个互相垂直轴转动实现单晶的NMR测量。作为典型的应用例子,本文利用该探头实现了单晶DGO(2NH2CH2COOH·H2C2O4)屏蔽张量的测量。 相似文献
4.
高效凝胶渗透色谱测定硅油分子量及其分布 总被引:1,自引:0,他引:1
采用HPGPC与蒸汽压渗透(VPO)结合的方法研究了甲基苯基硅油等分子量及其分布测定方法。 相似文献
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The viscosities of aqueous solutions of gelatin at different temperatures were carefully measured in a common glass‐capillary Ubbelohde viscometer at dilute to extremely dilute concentrations. The adsorption effect that occurred in the viscosity measurements was theoretically analyzed and discussed. A theory based on Langmuir isotherms could adequately describe the existing data. Some structural information was obtained by the use of an iterative fitting procedure to treat the reduced viscosity data, which disclosed that individual gelatin chains underwent a coil‐to‐helix transition as the solution cooled from 40 to 15 °C. © 2006 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 44: 1804–1812, 2006 相似文献
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In this paper an algorithm is developed to generate all nondominated extreme points and edges of the set of objective values of a multiple objective linear program. The approach uses simplex tableaux but avoids generating unnecessary extreme points or bases of extreme points. The procedure is based on, and improves, an algorithm Dauer and Liu developed for this problem. Essential to this approach is the work of Gal and Kruse on the neighborhood problem of determining all extreme points of a convex polytope that are adjacent to a given (degenerate) extreme point of the set. The algorithm will incorporate Gal's degeneracy graph approach to the neighborhood problem with Dauer's objective space analysis of multiple objective linear programs. 相似文献
9.
We employ dissipative particle dynamics (DPD) to examine the self‐assembly behavior of A2‐star‐(B‐alt‐C) molecules. We successfully observe various types of hierarchical structure‐within‐structures, such as A‐formed spheres in the matrix formed by B and C alternating layers, hexagonally packed A‐formed cylinders in the matrix with B and C segregated layers, B and C alternating layers‐within‐lamellae, coaxial B and C alternating domains within hexagonally packed BC‐formed cylinders in the A‐matrix, and co‐centric BC‐alternating domains within BC‐formed spheres in the A‐matrix, by increasing the A composition. Generally speaking, the small length‐scale B and C segregated domains are in parallel to the large length‐scale structures. This hierarchical periodicity along the same axis as well as the various characteristic structures, that the A2‐star‐(B‐alt‐C) copolymers display, are quite different from those in A‐block‐(B‐graft‐C) coil‐comb copolymers. Moreover, it is interesting to find that when the copolymer chain length increases, though the hierarchical structure type is maintained, the number of small length‐scale lamellae that can form within the large length‐scale structure increases. These hierarchical structures under various compositions are reported theoretically for the first time in the copolymer systems consisting of the alternating blocks, and are in good agreement with the most recent experimental work by Matsushita and co‐workers (Macromolecules 2007 , 40, 4023). 相似文献
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