1995年石河子地区冬春小麦生长期间气象条件分析

<正> 1995年石河子地区种植的46.26万亩冬春小麦喜获丰收,总产比1994年增长了35%,平均亩产289公斤,比1993年的270公斤增加了19公斤,单产总产均创历史最好记录,涌现出了122、142、143、总场、148、151、152等四亩产超过300公斤的团场,石总场万亩小麦超过500公斤高产田,现仅从气象条件对小麦生长发育的利弊影响作分析,为今后冬春小麦的生长期间合理管理提供了可靠的依据.     

补充供水对半干旱区旱地春小麦水分亏缺的补偿效应研究

大田试验设１１个水分处理，包括不灌溉（对照）和灌１、２次水、灌时期分别为春小麦三叶期、拨节期、抽穗斯和开花期。试验结果表明：三叶期灌溉的４个处理消除了苗期水分亏缺，通过提高小穗和数穗粒数而明显增产。     

春小麦产量及穗部主要性状的配合力分析

用选定的8个春小麦亲本进行4×4不完全双列杂交,对所得的F1代的产量等9个性状配合力进行分析.结果表明,各性状的一般配合力效应值与表型值之间都存在极显著正相关且在16个杂交组合间存在由基因型引起的真实遗传差异,其中,产量、株高、主穗长度、蛋白质含量在杂交组合间的表现差异是加性基因和非加性基因共同作用的结果,有效穗数、单株粒数、单株粒重是由非加性基因决定的.父本组对主穗小穗数的影响较大,母本组对千粒重的影响较大.各性状对产量的影响比较大而且都是正面影响,对蛋白质含量的影响较小,且有负面影响.母本格莱尼、毛娃娃与父本陕西超大穗、青春533的一般配合力综合效应值高,杂交组合互助红×阿勃、毛娃娃×陕西超大穗特殊配合力综合效应值高于其他组合.     

Cloning, Expression and Purification of Wheat Acetyl-CoA Carboxylases CT Domain in E.coil

The entire gene of carboxyltransferase(CT) domain of acetyl-CoA carboxylase(ACCase) from Chinese Spring wheat(CSW) plastid was cloned firstly,and the 2.3 kb gene was inserted into PET28a+ vector and expressed in E.coil in a soluble state.The (His)6 fusion protein was identified by SDS-PAGE and Western blot.The recombinant protein was purified by affinity chromatography,and the calculated molecular mass(Mr) was 88000.The results of the sequence analysis indicate that the cloned gene(GeneBank accession No.EU124675) was a supplement and revision of the reported ACCase CT partial cDNA from Chinese Spring wheat plastid.The recombinant protein will be significant for us to investigate the recognizing mechanism between ACCase and herbicides,and further to screen new herbicides.     

春小麦品种Waxy蛋白亚基的电泳检测

淀粉粒束缚淀粉合成酶,亦称蜡质蛋白（Waxy蛋白）,是合成禾谷类作物胚乳直链淀粉的关键酶。直链淀粉的含量大小对小麦的最终用途影响较大,特别对面条品质的影响尤为重要。本实验用SDS-PAGE电泳方法对国内外115份春小麦品种（系）Waxy蛋白进行了鉴定,从中鉴定出Wx—B1亚基缺失材料16份;Wx—D1亚基缺失材料2份;而全部材料都含有Wx—A1亚基。3个亚基出现频率为Wx—A1〉Wx-D1〉Wx—B1。在所检测材料中没有发现3个亚基都缺失的或其中2个亚基缺失的类型。在缺失Wx—B1亚基的材料中以新疆的春小麦品种居多,占缺失Wx-B1亚基材料的56．3％。     

Cloning, Expression and Purification of Wheat Acetyl-CoA Carboxylases CT Domain in E. coil

The entire gene of carboxyltransferase（CT） domain of acetyl-CoA carboxylase（ACCase） from Chinese Spring wheat（CSW） plastid was cloned firstly, and the 2.3 kb gene was inserted into PET28a^＋ vector and expressed in E. coil in a soluble state. The （His）6 fusion protein was identified by SDS-PAGE and Western blot. The recombinant protein was purified by affinity chromatography, and the calculated molecular mass（Mr） was 88000. The results of the sequence analysis indicate that the cloned gene（GeneBank accession No. EU124675） was a supplement and revision of the reported ACCase CT partial cDNA from Chinese Spring wheat plastid. The recombinant protein will be significant for us to investigate the recognizing mechanism between ACCase and herbicides, and further to screen new herbicides.     

三十烷醇对春小麦的生理效应

本文探讨了三十烷醇对春小麦品种赛洛斯的生理效应,试验研究说明:三十烷醇对春小麦的生长量、叶绿素含量、硝酸还原酶和性细胞透性方面,均有明显的生理效应,为在大田应用三十烷醇提供了一定的理论依据。从试验选定的三个处理浓度试验结果比较来看,以0.5ppm为最佳。