Amine containing cationic methacrylate copolymers as efficient gene delivery vehicles to retinal epithelial cells

Non‐viral gene delivery vectors have emerged as potential alternatives in the field of gene therapy by replacing the biological viral vectors. DNA–cationic polymer complexes are one of the most promising systems to target many inborn or acquired diseases without the utilization of conventional drugs. Despite the excellent binding efficiency of cationic polymers, the gene transfection seems limited to date. In this work, a series of ammonium‐based block‐copolymers with different alkyl side chains (ethyl, butyl, and hexyl) and functionality (alcohol, amine, and alkyl) have been prepared to evaluate their capacity to deliver genetic material. First, different ionic liquid monomers with different pendent functional groups were prepared and characterized. Then, polyplexes elaborated with different polymers at several polymer DNA ratios (w/w) were characterized in terms of size, zeta potential, and DNA binding, release, and protection capacity. Finally, the transfection efficiency and cell viability was evaluated in ARPE19 cells. We found that only the systems containing the amine pendent group were able to transfect ARPE19 cell and, that this amine containing polymer was less cytotoxic even at high polymer/DNA ratios (30:1). In conclusion, our studies suggested that the proper selection of the pendent group substantially impacts overall transfection efficiency of cationic polymers. © 2016 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2017 , 55, 280–287     

Novel materials for bioanalytical and biomedical applications: Environmental response and binding/release capabilities of amphiphilic hydrogels with shape‐persistent dendritic junctions

The binding and release capabilities of a hydrogel series, constructed of hydrophilic poly(ethylene glycol) segments and hydrophobic dendritic junctions [poly (benzyl ether)s], are evaluated in aqueous media. The environmental response of the amphiphilic networks is also tested in water at three pH values: 1.5, 7.0, and 10.1. The highest swelling ratio is observed under acidic conditions and varies between 3.7 and 6.5, depending on the crosslinking density and dendrimer generation. Gel specimens with embedded indicators react within 3–6 s with a clear color switch to the change in the pH of the surrounding medium. The experiments with model anionic and cationic indicators and stains show that the hydrogels have basic interiors. The gel binding capabilities depend on the water solubility of the substrate and on the size of the incorporated dendritic fragments. Model release studies have been performed at 37 °C and pHs 1.5, 7.0, and 10.1. The observed phenomena are explained by the transformations in the structure and charge that both the networks and the model compounds undergo with the changes in the pH of the aqueous medium. © 2005 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 43: 4017–4029, 2005     

本地应用服务交付平台的应用场景和总体要求

为适应宽带有线电视家庭网络上智能电视业务的融合与创新发展需要,研究了家庭网络本地应用服务交付平台(LASDP)的使用场景和总体要求.主要工作包括本地应用交付平台的总体描述、应用场景参考架构、应用服务场景和总体要求4部分.开放、标准和敏捷的本地应用服务交付平台,将有益于家庭用户、应用服务提供商和有线玩过运营商.     

基于融合CDN构建未来智能内容管道

详细探讨了传统CDN向融合CDN的演进方案、融合CDN构建智能内容管道的方法及在管道中的作用.为了更好地支撑业务发展,融合CDN需要与承载网络相互渗透,具备网络感知、业务感知、用户感知三大能力.为了保障移动业务的更好体验,提出CDN的网络位置应该下移到无线网络中,并根据无线网络的特性进行适配和优化.     

Quantifying the effect of local interconnects on on-chip power distribution

Existing methods to analyze and optimize on-chip power distribution networks typically focus only on global power network modeled as a two-dimensional mesh. In practice, current is supplied to switching transistors through a local power network at the lower metal layers. The local power network is connected to a global network through a stack of vias. The effect of these vias and the resistance of the local power network are typically ignored when optimizing a power network and placing decoupling capacitors. By modeling the power distribution network as a three-dimensional mesh, the error due to ignoring via and local interconnect resistances is quantified. It is demonstrated that ignoring the local power network and vias can both underestimate (by up to 45%) or overestimate (by up to 50%) the effective resistance of a power distribution network. The error depends upon multiple parameters such as the width of local and global power lines and via resistance. A design space is also generated to indicate the valid width of local and global power lines where the target resistance is satisfied. It is shown that a wider global network can be used to obtain a narrower local network, providing additional flexibility in the physical design process since routability is an important concern at lower metal layers. At high via resistances, however, this approach causes significant increase in the width of a global power network, indicating the growing significance of local power network and vias.