Homotopy of the classical configuration space for the two-magnon sector of a magnetic Heisenberg ring

A finite Heisenberg magnetic ring with an arbitrary single-node spin and two spin deviations from the ferromagnetic saturation is considered as the system of two Bethe pseudoparticles. The set of all relevant magnetic configurations spans a surface which can be recognised as a Mőbius strip. The dynamics of the system imposes the double twist of all regular orbits of the translation symmetry group.     

基于Web平台的机器翻译系统

用户可以通过Internet访问和使用机器翻译系统，在线机器翻译系统可以帮助用户阅读网上外文材料。用户一般通过购买相关软件或访问提供在线翻译的网站来获取翻译服务，但是专用软件价格昂贵，或者获得的在线翻译效果不能令人满意。通过Web服务与J2EE技术的结合，提出了将机器翻译拳统包装为Web服务的机制，使企业和个人能够以较低的代价方便地享受更高质量的翻译服务。     

Finite-size scaling corrections for the eight-vertex model

Finite-size scaling corrections are calculated analytically for two of the maximal eigenvalues of the transfer matrix in the isotropic eight-vertex model. The valuec=1 for the conformal anomaly of the Virasoro algebra is confirmed.     

Density-and-temperature-dependent volume translation for the SRK EOS: 1. Pure fluids

A mathematical framework for applying a density-and-temperature-dependent volume translation in a thermodynamically consistent manner was developed. Volumetric equations of state (EOS)s that incorporate this translation procedure can be used to generate derived properties, such as fugacity and enthalpy departure, that are based on isothermal departure or residuals from ideal gas state conditions. This kind of translation serves to improve the original EOS and not simply act as a correlation for molar volumes. A density-and-temperature-modified translation of this type was applied to the Soave–Redlich–Kwong EOS and was shown to possess accuracy for saturation pressure predictions equivalent to the untranslated EOS, as well as greatly improved density predictions compared to what is available when using only constant valued translation. The EOS translated in this manner retains many of the important features of the untranslated EOS, such as explicit calculation of volume roots, while having the representation capabilities of substantially more complicated models, such as the extended virial equation of Benedict, Webb, Rubin, and Starling.     

A new size extensive multireference perturbation theory

A new multireference perturbation series is derived based on the Rayleigh–Schrödinger perturbation theory. It is orbitally invariant. Its computational cost is comparable to the single reference Møller–Plesset perturbation theory. It is demonstrated numerically that the present multireference second‐ and third‐order energies are size extensive by two types of supermolecules composed of H₂ and BH monomers. Spectroscopic constants of as well as the ground state energies of H₂O, NH₂, and CH₂ at three bond lengths have been calculated with the second multireference perturbation theory. The dissociation behaviors of CH₄ and HF have also been investigated. Comparisons with other approximate theoretical models as well as the experimental data have been carried out to show their relative performances. © 2013 Wiley Periodicals, Inc.     

Chemical Evolution of a Bacterial Proteome

We have changed the amino acid set of the genetic code of Escherichia coli by evolving cultures capable of growing on the synthetic noncanonical amino acid L ‐β‐(thieno[3,2‐b]pyrrolyl)alanine ([3,2]Tpa) as a sole surrogate for the canonical amino acid L ‐tryptophan (Trp). A long‐term cultivation experiment in defined synthetic media resulted in the evolution of cells capable of surviving Trp→[3,2]Tpa substitutions in their proteomes in response to the 20 899 TGG codons of the E. coli W3110 genome. These evolved bacteria with new‐to‐nature amino acid composition showed robust growth in the complete absence of Trp. Our experimental results illustrate an approach for the evolution of synthetic cells with alternative biochemical building blocks.     

Initiation of Protein Synthesis with Non‐Canonical Amino Acids In Vivo

By transplanting identity elements into E. coli tRNA^fMet, we have engineered an orthogonal initiator tRNA (itRNA^Ty2) that is a substrate for Methanocaldococcus jannaschii TyrRS. We demonstrate that itRNA^Ty2 can initiate translation in vivo with aromatic non‐canonical amino acids (ncAAs) bearing diverse sidechains. Although the initial system suffered from low yields, deleting redundant copies of tRNA^fMet from the genome afforded an E. coli strain in which the efficiency of non‐canonical initiation equals elongation. With this improved system we produced a protein containing two distinct ncAAs at the first and second positions, an initial step towards producing completely unnatural polypeptides in vivo. This work provides a valuable tool to synthetic biology and demonstrates remarkable versatility of the E. coli translational machinery for initiation with ncAAs in vivo.     

In vitro translation with [<Superscript>34</Superscript>S]-labeled methionine,selenomethionine, and telluromethionine

Heteroisotope and heteroatom tagging with [³⁴S]-enriched methionine (Met), selenomethionine (SeMet), and telluromethionine (TeMet) was applied to in vitro translation. Green fluorescent protein (GFP) and JNK stimulatory phosphatase-1 (JSP-1) genes were translated with wheat germ extract (WGE) in the presence of Met derivatives. GFPs containing Met derivatives were subjected to HPLC coupled with treble detection, i.e., a photodiode array detector, a fluorescence detector, and an inductively coupled plasma mass spectrometer (ICP-MS). The activities of JSP-1-containing Met derivatives were also measured. GFP and JSP-1 containing [³⁴S]-Met and SeMet showed comparable fluorescence intensities and enzyme activities to those containing naturally occurring Met. TeMet was unstable and decomposed in WGE, whereas SeMet was stable throughout the experimental period. Thus, although Te was the most sensitive to ICP-MS detection among S, Se, and Te, TeMet was less incorporated into the proteins than Met and SeMet. Finally, the potential of heteroisotope and heteroatom tagging of desired proteins in in vitro translation followed by ICP-MS detection was discussed. Figure TeMet was less incorporated into GFP than Met and SeMet due to its instability in WGE