Initiation of Protein Synthesis with Non‐Canonical Amino Acids In Vivo

By transplanting identity elements into E. coli tRNA^fMet, we have engineered an orthogonal initiator tRNA (itRNA^Ty2) that is a substrate for Methanocaldococcus jannaschii TyrRS. We demonstrate that itRNA^Ty2 can initiate translation in vivo with aromatic non‐canonical amino acids (ncAAs) bearing diverse sidechains. Although the initial system suffered from low yields, deleting redundant copies of tRNA^fMet from the genome afforded an E. coli strain in which the efficiency of non‐canonical initiation equals elongation. With this improved system we produced a protein containing two distinct ncAAs at the first and second positions, an initial step towards producing completely unnatural polypeptides in vivo. This work provides a valuable tool to synthetic biology and demonstrates remarkable versatility of the E. coli translational machinery for initiation with ncAAs in vivo.     

五阶势MKdV方程的一个不变性

§1Introduction Asweknow,Backlundtransformation[1-3]isaverypowerfulwayforfindingnonline evolutionequations.Inrecentdecades,Painlevéanalysis[4]hasbecomeaverypopu methodtoobtainBacklundtransformation.Inreference[5],fordevelopingthetheory Painlevéanalysis,AndrewPickeringintroduceanewexpansionvariableZwhichsatisf thefollowingRicattiSystem:Zx=1-AZ-BZ2,Zt=-C+(AC+Cx)Z-(D-BC)Z2.(1.Astheapplicationofthenewexpansionvaraible,thepotentialfifth-orderMKd equation(PMKdV5)-vxt+(vxxxxx-10k2v2…     

基于交比不变性的投影仪标定

提出了一种新的投影仪标定方法以提高数字光栅投影三维测量中投影仪标定的准确性.该方法结合二次投影技术和交比不变性进行投影仪标定.采用二次投影技术解决投射图案与标定板图案互相干扰的问题;采用交比不变性以避免引入相机的标定误差.接着进行了对比实验,以验证所提方法的有效性.选取需要相机参数的传统投影仪标定方法以及根据全局单应性...     

基于方差约束与几何不变特性的图像匹配算法

为解决较多图像匹配算法主要通过测量关键点之间的距离来实现特征匹配,忽略了图像的结构信息,使其存在较多的误匹配的问题,本文设计了方差约束耦合几何不变特性的图像匹配算法。借助于Forstner算子计算像素点的兴趣值,以检测图像的特征;计算图像的梯度信息,获取图像的方向值,并切割图像特征的圆形邻域,从而获取扇形子域;以图像的方向值为基础,通过计算扇形子域中的灰度不变矩,输出对应的特征向量;引入区域方差函数,获取图像的结构信息,将其加入至图像特征的匹配过程,以约束欧式距离的测量结果,实现图像特征匹配;最后利用匹配点间的几何不变特性,对匹配特征去伪求真,优化匹配结果。测试数据表明：相对于已有的匹配技术,在对无变换图像、缩放图像以及旋转图像匹配时,所提算法拥有更高的匹配准确度,分别达到了96.56%、95.38%和93.52%。     

On the invariant translation approximation property for discrete groups

Recently J.Roe considered the question of whether for a discrete group the reduced group -algebra is the fixed point algebra of Ad acting on the uniform Roe algebra . is said to have the invariant translation approximation property in this case. We consider a slight generalization of this property which, for exact , is equivalent to the operator space approximation property of . We also give a new characterization of exactness and a short proof of the equivalence of exactness of and exactness of for discrete groups.

     

一种利用旋转结构的分布式信号DOA估计方法

某些领域中由于多径影响信号往往具有分布特性,此时传统波达方向(DOA)参数估计算法往往失效.针对此类问题提出了一种无需搜索的分布式信号DOA估计算法,该方法利用已知信号有用信息逐一估计分布信号在相邻阵元间产生的旋转因子,利用DOA旋转不变结构估计分布目标DOA参数.与点目标ESPRIT方法相比该方法能够有效克服多径扩展影响,不敏感于多径分布的具体形式,并在一定程度上抑制相位误差影响.仿真实验证明了其有效性.     

ISAR成像中一种距离对准的新方法

本文采用一维距离像相关模型,提出一种ISAR成像中距离对准的新方法,该方法充分考虑了一维距离像间的直流漂移和归一化系数,且具有较好的对准效果,通过实测数据验证了该方法的有效性。     

相干视频地杂波模拟的通用仿真模型

针对相干视频地杂波模拟中非高斯相关随机序列的产生,提出一种通用的仿真模型。使用迭代算法和随机向量法完成零记忆非线性变换过程,迭代算法用来计算非线性变换过程中高斯序列相关性的变化,而随机向量法用来完成线性变换,仿真实验数据证明利用这两种方法可精确地产生具有指定概率密度和功率谱的非高斯相关随机序列,逼真地复现既包含振幅又包含相位的雷达相干视频散射信号。同时给出了迭代算法使用过程中的注意问题及地杂波模型仿真的过程。     

Non-Noether conserved quantity constructed by using form invariance for Birkhoffian system

Based on the invariance of Birkhoffian equations under the infinitesimal transformations of groups, the definition and the criterion of a form invariance for a Birkhoffian system are established. The condition under which the form invariance can lead to a non-Noether conserved quantity and the form of the conserved quantity are deduced by relyingon the total time derivative along the trajectory of the equations, and two corollaries in special cases are presented. An example is finally given to illustrate the application of the results.     

语言计量研究中的上小三角矩阵压缩存储算法

三角矩阵的压缩存储是科学研究和工程计算领域经常遇到的问题。在语言计量研究的实际应用当中,需要用到一类主对角线元素全为零的特殊三角矩阵川、三角矩阵。通过对相关压缩算法的考察,给出了上小三角矩阵中行主顺序压缩存储算法的正向公式和逆向公式,还给出了算法在语言计量研究中的应用。基于算法的后续计量研究将有助于进一步揭示人类语言结构与演化的规律。