High-resolution Hadamard transform microscope fluorescence imaging: quantifying the DNA content in single cells

This study presents a novel miniaturized Hadamard transform fluorescence imaging microscope, by combining a conventional fluorescence microscope with Hadamard transform multiplexing encoding using a one-dimensional movable mask to realize spatial resolution and a linear CCD for multichannel detection. The microscope can provide high-resolution automatically-generated 0–255 gray level HT images for morphological analysis and visualization of a single cell, and normalized HT images for cellular quantitative measurements. The microscopes imaging capability was applied to measure the DNA content in human lymphocyte, chicken erythrocyte and eel erythrocyte, and a comparative study was performed. The results show that the calibrated DNA content in a chicken erythrocyte is 2.32 pg when human lymphocyte is used as the standard, and eel erythrocyte may be a potentially reliable and novel standard for determining DNA contents in other species because it has a stable DNA value of 2.06 pg, with a CV of 4.3% when 20 eel erythrocytes are measured. The results also demonstrate that the HT imaging microscope should be valuable in the fields of medicine and cell biology.     

顺磁性聚酯金属配合物的合成及其驰豫性能的研究

本文通过二乙三胺五乙酸（ＤＴＰＡ）或乙二胺四乙酸（ＥＤＴＡ）的双酸酐与二元醇或二元酚进行聚合反应，制备了两个系列共１５种新的聚酯型大分子配体及其顺磁性金属配合物，用核磁、红外光谱以及元素分析表征了配体和配合物的结构。初步试验结果表明，与相应的小分子金属配合物相比，聚酯金属配合物具有较高的弛豫性能。     

The participation of the anion and alkyl substituent of diaryliodonium salts in photo‐initiated cationic polymerization reactions

The photo‐initiated cationic polymerization (PCP) of epoxides using diaryliodonium salt photoacid generators (PAGs) bearing alkyl groups and anions was investigated. The properties and reactivities of a series of iodonium salts containing various cations and anions were compared in the context of a PCP reaction. The products from the decomposition of the cations of these salts were analyzed using gas chromatography‐mass spectrometry (GC‐MS) spectra. The relationship between the molecular structure of the salts and their reaction mechanism in the PCP reaction was investigated. Based on the results of the investigation, it was concluded that the structures of the cations and anions of theiodonium salts affect the PCP reaction rate, which was controlled by the products from the diaryliodonium salts. As part of an additional investigation, the diaryliodonium salts‐epoxide materials were applied to 254 nm‐photo‐patterning. Copyright © 2006 John Wiley & Sons, Ltd.     

LyP-1 peptide-functionalized gold nanoprisms for SERRS imaging and tumor growth suppressing by PTT induced-hyperthermia

The gold nanoprisms (GNPs) have exhibited special plasmonic properties for biomedical applications because of their unique shapes and dimensions. Based on their optical performance, the NIR dye IR780 not only enabled the GNPs-based nanosystem as SERRS nanoparticles for Raman-encoded molecular imaging, but also enhanced the plasmonic photothermal property by laser irradiation. Meanwhile, the GNPs/IR780-Lyp-1 by introduction of tumor-homing peptide segment LyP-1, which presents high affinity to p32 protein, demonstrated the increased enrichment in tumor region and enhanced photothermal therapy efficacy.     

Photochemical Ionic-Conductivity Switching Systems of Photochromic Crown Ethers for Information Technology

Design of two types of ion-conducting systems using photochromic crown ethers as the photocontrol agents is described; one type is based on the phase transition of azobenzene derivatives induced by their photoisomerization and the other based on the molecular control of metal ion complexation by crowned spirobenzopyrans. The photoresponsive ion-conducting systems are applicable to electrostatic imaging and photorefractive materials.     

抛物型等效法研究指数型梯度折射率纤维的成像

本文采用抛物型等效法研究指数型梯度折射率纤维的成像特性。这种方法计算比严格解析法方便,精度比锥形等效法高,且适用范围大。     

太赫兹网络中基于优先级调度的低开销双信道MAC协议

针对现有太赫兹通信网络双信道MAC协议存在控制开销冗余和在竞争信道时缺少优先级调度策略等问题,提出了一种太赫兹网络中基于优先级调度的低开销双信道MAC协议（Low Overhead Dual-channel MAC Protocol Based on Priority Scheduling,LO-PSMAC）,包含通信距离预判、优先级调度策略的CSMA/CA和精简THz频段MAC帧三种机制,可有效提升信道利用率和整体网络吞吐量,同时减小控制开销和降低数据平均时延。仿真结果表明,所提协议与现有太赫兹双信道MAC协议相比,MAC层吞吐量和信道利用率分别提升了7.14%和14.75%,数据平均时延降低了14.21%。     

335 GHz非平衡式肖特基二极管三倍频器

基于混合集成的方式,采用对称锥形渐变线匹配结构设计了335 GHz非平衡式三倍频器。在保证单模传输的条件下,该匹配结构不仅能够固定二极管位置,而且可以增大匹配效果,解决了高频段倍频器3 dB带宽较窄的问题。实测结果表明,该倍频器在330~356 GHz频率范围内输出功率均大于5 mW。驱动功率为220 mW时,有最高输出功率11.2 mW,由它作为核心器件组成的固态太赫兹本振源,能够驱动超外差接收机中670 GHz二次谐波混频器。     

基于拓扑绝缘体异质结的宽带太赫兹探测器

二维材料中的新量子态对凝聚态物理和现代光电器件的发展具有重要意义。然而具有宽带、室温和快速响应能力的太赫兹光电探测技术,由于缺乏暗电流和光吸收之间的最佳平衡,仍然面临着巨大的挑战。在这项研究中,作者合成了新型拓扑绝缘体材料GeBi₄Te₇,并搭建了其与Bi₂Te₃的范德华异质结,以实现高灵敏度的太赫兹光电探测器。在平面金属-材料-金属结构中实现了在室温下将低光子能量太赫兹波段直接转化为光电流。结果表明,基于Bi₂Te₃-GeBi₄Te₇的太赫兹光电探测器能够实现0.02～0.54 THz的宽谱探测,且具有很高的光响应率(在0.112、0.27、0.5 THz下分别为592 V·W^-1、203 V·W^-1、40 V·W^-1),响应时间小于6μs。值得注意的是,它被用于高频太赫兹的成像应用演示。这些结果为Bi₂Te₃     

基于直线运动机构的大变倍比红外变焦成像系统

针对同时兼顾大范围搜索和精确识别目标的迫切需求,研制了一种大变倍比红外变焦成像系统,设计两片独立运动的变倍镜及一片补偿镜,通过两个变倍镜级联的方式获得大变倍比。结合系统运动镜片多及变焦曲线复杂的特点,采用直线运动机构实现镜片变焦运动,使用集成编码器及螺纹丝杆的直线电机作为驱动。通过有限元仿真开展了系统力学分析,所设计镜片最大位移为3.04×10^-3 mm。成像系统适用于中波红外制冷式640×512焦平面阵列探测器,变倍比达到55倍。实验室成像及外场实景成像的结果表明,系统在焦距由6 mm至330 mm连续变化的过程中成像清晰、像质良好,验证了系统的连续变焦成像性能,该设计合理可靠。研究成果在搜索、跟踪、侦察、监视等方面有广阔的应用前景。