Comparison between two different derivations of the differential equations for free vibration of the ideal fluid column in a communication tube

How would the ideal fluid column in a communication tube moves, the present paper presents two different solutions, one of which has been existing in the literature not less than a half century, while the second is recommended by us with this article. We have deduced the differential equation anew and given the main motion features.According to our opinion, the hitherto, for a long time broadly used, derivation is not undisputable. Noticeably, the results of our new derivation can duely degenerate into Newton-, John Bernoulli- and Daniel Bernoulli-period laws of vibration.     

不同激光参数照射下血管中血栓的演变

目前,激光是治疗葡萄酒色斑(Port Wine Stain,PWS)最有效的疗法.然而,由于选择性光热效应机理研究的欠缺,PWS的临床彻底清除率依然很低(＜20％).本文利用鼠脊视窗模型研究了不同激光参数照射下血管中光凝块和血栓的演变规律,以期为开发新的治疗策略提供依据.实验结果表明,Nd:YAG激光(1064 nm)...     

A simple,time‐saving,microwave‐assisted periodic acid–Schiff´s staining of glycoproteins on 1D electrophoretic gels

We introduce an optimized periodic acid–Schiff´s staining of glycoproteins on 1D electrophoretic gels. Thanks to heating in a household microwave oven the protocol of standard periodic acid–Schiff´s staining has been accelerated from 6 h to below 10 min employing standard chemistry. At the same time, we show that the microwave‐assisted glycoprotein staining is at least as sensitive as the conventional approach. All glycoproteins stained by the microwave‐accelerated procedure were successfully identified using MALDI TOF/TOF mass spectrometry. The ensuing reduction in gel staining time and simplification of the staining protocol should significantly increase laboratory throughput when glycoprotein detection on electrophoretic gels is required in large numbers.     

导热油油垢清洗研究

本文综述了导热油的种类;应用时存在的不足——结垢,结垢的原因;目前国内外清除结垢的清洗剂的种类以及清洗工艺;并提出清洗焦油垢的方向。     

Adaptive Chemical Networks under Non‐Equilibrium Conditions: The Evaporating Droplet

Non‐volatile solutes in an evaporating drop experience an out‐of‐equilibrium state due to non‐linear concentration effects and complex flow patterns. Here, we demonstrate a small molecule chemical reaction network that undergoes a rapid adaptation response to the out‐of‐equilibrium conditions inside the droplet leading to control over the molecular constitution and spatial arrangement of the deposition pattern. Adaptation results in a pronounced coffee stain effect and coupling to chemical concentration gradients within the drop is demonstrated. Amplification and suppression of network species are readily identifiable with confocal fluorescence microscopy. We anticipate that these observations will contribute to the design and exploration of out‐of‐equilibrium chemical systems, as well as be useful towards the development of point‐of‐care medical diagnostics and controlled deposition of small molecules through inkjet printing.     

一种新的混合斑精子分离方法

制备了精子表面膜抗原受精素β蛋白(Fertilinβ)的特异性抗体,通过抗原-抗体反应和抗体固相偶联技术,将抗体连接到琼脂糖球珠上,建立了混合斑精子分离纯化的新方法.首先,通过聚合酶链式反应(PCR)扩增编码人受精素β蛋白第341~373位氨基酸的基因序列,构建PGEX-4T-1/Fertilinβ原核表达质粒;其次,诱导表达GST-Fertilinβ融合蛋白,制备受精素β蛋白多克隆抗体,免疫荧光检测结果表明,受精素β蛋白定位于精子的头后部,并且阴道上皮细胞没有受精素β蛋白的表达;最后,将受精素β抗体与ProteinA琼脂糖球珠连接构建固相抗体系统,将精子吸附于表面,可从混合斑(精子与阴道上皮细胞混合液)中分离纯化精子.本文为性犯罪案件侦破提供了新的方法.     

High‐throughput negative detection of SDS‐PAGE separated proteins and its application for proteomics

A negative detection method for proteins on SDS‐PAGE is described. In this method, Eosin Y (EY) was selectively precipitated in the gel background, which is absent from those zones where proteins are located through the formation of a stable water‐soluble protein–dye complex. Negative staining of proteins using EY, allows high‐sensitivity, low‐cost, and simple protocol. The new described method takes less than an hour to complete all the protocol, with a detection limit of 0.5 ng of single protein band. Comparing with imidazole‐zinc negative stain, EY dye provides broader linear dynamic range, higher sensitivity and reproducibility, and better obvious contrast between the protein bands or spots and background. Furthermore, the novel technique developed here presented a real practical method for simultaneous processing of multiple gels, which makes it possible to perform high‐throughput staining for proteome research. Additionally, we have also compared the influence of staining method on the quality of mass spectra by PMF.     

鲜红斑痣光谱测定系统与临床实验

对进行激光治疗的鲜红斑痣患者的皮肤进行光谱定量分析,有利于正确分析患者病变皮肤的光学特性、调整手术设备的工作参数和提高激光治疗的效果。基于对鲜红斑痣形成机理分析及激光临床治疗过程观察,文章建立了一套自动数据采集和分析的光谱系统,可以用于测定鲜红斑痣皮肤微区在380～780 nm范围内的光谱,光谱分辨率为1 nm。实验可测定不同年龄、不同色素痣的光谱曲线,分析并讨论了影响系统工作的原因和解决方法。     

鲜红斑痣光动力治疗的荧光光谱监测

应用荧光光谱技术监测了鲜红斑痣(PWS)光动力治疗(PDT)中的光敏剂血药浓度与光产物生成。以532 nm倍频Nd∶YAG激光器作为PDT照射光源与荧光激发光源,以光谱仪与ICCD采集荧光光谱。在系统验证实验中,构建含有血卟啉单甲醚(HMME)的小鼠正常皮肤的荧光基本光谱,通过最小二乘光谱拟合区分HMME荧光(624 nm)与光产物荧光(652 nm)。含有PSD-007的病人患区荧光光谱拟合采用相同基本光谱,获得不同病人个体具有显著差异的光敏剂血药浓度曲线与光产物生成漂白曲线。所建立的荧光光谱监测系统与光谱拟合方法可为PDT精确量化剂量学方法的建立提供技术手段,所得结果有利于制定个性化的PDT治疗方案。     

激光与强脉冲光治疗皮肤鲜红斑痣的现状与进展

本文综述了近年来国内外使用激光与强脉冲光治疗皮肤血管性病变鲜红斑痣的现状及研究进展,并对其治疗方法与并发症进行了分析,为提高治愈率和减少副作用提供了临床治疗依据。