聚焦光声鲜红斑痣诊断技术的模拟实验研究

对鲜红斑痣皮肤(PWS,port wine stain)的客观评估是应用激光成功治疗PWS的关键.本文提出了一种基于聚焦换能器的光声深度分辨技术来实现对PWS皮肤的评估.通过利用聚焦换能器,该技术不但可以实现定位病灶位置的目的,还可以减少非聚焦区光声信号的干扰.模拟样品测试结果表明,聚焦光声深度分辨技术可实现对PWS皮肤的病灶深度、表皮黑色索含量以及病变层厚度的有效检测.初步的实验结果显示了该技术在PWS皮肤诊断方面的应用前景.     

侵染西瓜的黄瓜绿斑驳花叶病毒快速检测

黄瓜绿斑驳花叶病毒（ CGMMV）是危害西瓜生产的重要病害,造成西瓜果实腐烂,失去商业价值。应用透射电子显微镜和单抗dot?ELISA技术对采自浙江温岭的西瓜病叶进行快速病原检测,电镜负染色观察到病株汁液中存在大量刚直杆状的病毒粒子,超薄切片观察到细胞质内存在整齐排列的杆状病毒聚集体,与烟草花叶病毒属（ Tobamovirus）的形态特征相符;进一步采用dot?ELISA技术进行血清学检测,显示西瓜病叶与CGMMV特异性抗体呈阳性反应,检测结果表明感染西瓜的杆状病毒为CGMMV。从而为侵染西瓜的CGMMV提供快速检测途径。     

光通量在血液中的分布研究

光通量在血液中的分布是激光医学研究所必须了解的.葡萄酒色痣是皮肤的常见病之一,而用激光治疗是经常使用的手段.设血液在整个皮肤内是均匀分布的,而不是封闭在离散的血管内,那么通常可模拟葡萄酒色痣的激光治疗.在这个假设条件下,计算和分析了它的结果.     

小剂量HpD配合可变脉宽532nm激光治疗鲜红斑痣的实验研究

目的:为寻求治疗鲜红斑痣疗效佳、对正常皮肤损伤小、副反应小的方法。方法:以不同剂量HpD配合不同剂量带同步冷却装置的532nm激光分成四个治疗组,照射鲜红斑痣动物模型来克享大公鸡鸡冠,并与单纯激光组比较,激光治疗前及治疗后6周进行疗效评估。结果:5个组间疗效有非常显著的差异(p<0.01),治疗组的疗效优于对照组。四个治疗组间疗效有差异(p<0.05),提高HpD剂量可提高疗效。任一组能量密度组间疗效有差异(p<0.05),提高能量密度可提高疗效。结论:小剂量HpD配合可变脉宽532nm激光治疗鲜红斑痣可以提高疗效。提高能量密度和HpD剂量可提高疗效。     

An expanding negative detection method for the visualization of DNA in polyacrylamide gels by eosin Y

A sensitive and easy technique has been developed for the negative detection of DNA following PAGE using eosin Y. After electrophoresis, gels are fixed and stained within 40 min to provide a detection limit of 0.1-0.2 ng of single DNA band, which appears as transparent and colorless under the opaque gel matrix background. The sensitivity of the new stain is fourfold better than zinc-imidazole negative and ethidium bromide stains. Furthermore, the newly developed staining method has been successfully applied to RNA visualization in polyacrylamide gels. In addition, the inclusion of inorganic salts in staining solution was also investigated, which has great effect on the staining efficiency.     

Microcantilevers as gas‐phase sensing platforms: Simplification and optimization of the production of polymer coated porous‐silicon‐over‐silicon microcantilevers

The asymmetric roughening of silicon microcantilevers using different vapor stain‐etching conditions is studied with the aim of optimizing face selective coating of microcantilevers by polymers through simple dipping. The effect of roughening is studied by following the time‐dependent guest‐induced bending of silicone microcantilevers coated with a poly‐4‐vinylpyridine sensing layer. A correlation between the surface roughness of the microcantilevers and their time‐dependent guest‐induced bending is gained from combining high resolution scanning electron microscopy studies of the surface of the microcantilevers as well as their cross‐sections with time‐dependent guest‐induced microcantilever bending. The purpose of the present work is to lay the foundations for a small and relatively simple gas‐phase sensing tool based on a microcantilever platform capable of offering wide range sensing capabilities. © 2013 Wiley Periodicals, 2014 , 52, 141–146     

溴化亚铜脉冲激光光动力学疗法治疗鲜红斑痣临床研究

本采用省化亚铜脉冲激光学方法治疗鲜红斑痣并进行临床研究，共４０例，８４个区域，共疗效与病变类型、ＨＰＤ剂量、功率密度与能量关系予以阐述。     

铜蒸气激光与光敏剂联合治疗鲜红斑痣的临床研究

铜蒸气激光与光敏剂ＰＳＤ—００７进行ＰＤＴ治疗鲜红斑痣３４０例，总有效率达９８．８％其中优良率７６．６％，无遗留任何疤痕。与单纯铜激光或氩激光光源相比，其优率Ｐ＜０．０１     

A statistical comparison of silver and SYPRO Ruby staining for proteomic analysis

Silver staining has been the method most commonly employed for high sensitivity staining of proteins following two-dimensional gel electrophoresis. Whilst this method offers detection in the nanogram range it does have major drawbacks including a lack of linearity, nonstoichiometric staining of proteins, a lack of compatibility with the microchemical preparation of proteins for identification by mass spectrometric techniques, and a highly subjective assessment of the staining endpoint. SYPRO Ruby is a relatively new, ruthenium complex-based stain which is reported to offer advantages over silver, particularly in overcoming the limitations cited above. We describe a series of experiments where several protein staining procedures commonly employed are compared. To enable optimization of the in situ digestion procedure, a statistical approach has been undertaken. The effects of a variety of staining, digestion, and analysis protocols on the downstream processing of a test radiolabeled protein were studied. The data confirms that as well as offering sensitivity similar to silver, SYPRO Ruby staining is reproducible, linear, and offers a higher level of compatibility with the identification of proteins by mass spectrometry.     

A rapid and simple 8‐quinolinol‐based fluorescent stain of phosphoproteins in polyacrylamide gel after electrophoresis

In order to obtain an easy and rapid protocol to visualize phosphoproteins in SDS‐PAGE, a fluorescent detection method named 8‐Quinolinol (8‐Q) stain is described. 8‐Q can form ternary complexes in the gel matrix contributed by the affinity of aluminum ion (Al³⁺) to the phosphate groups on the proteins and the metal chelating property of 8‐Quinolinol, exhibiting strong fluorescence in ultraviolet light. It can visualize as little as 4～8 ng of α‐casein and β‐casein, 16～32 ng of ovalbumin and κ‐casein which is more sensitive than Stains‐All but less sensitive than Pro‐Q Diamond. The protocol of 8‐Q requires only 70 min in 0.75 mm mini‐size or 1.0 mm large‐size gels with five changes of solutions without destaining step; Pro‐Q takes at least 250 min with 11 changes of solutions. In addition, the new method was confirmed by the study of dephosphorylation and LC‐MS/MS, respectively. The approach to visualize phosphoprotein utilizing 8‐Q could be an alternative to simplify the analytical operations for phosphoproteomics research.