溴化亚铜脉冲激光光动力学疗法治疗鲜红斑痣临床研究

用ＣｕＢｒ脉冲激光光动力学疗法治疗鲜红斑痣８例２１个区域，其中１１个区红色完全褪去，７个区残留少量浅色，３个区红色明显褪去，２０个区域无疤痕形成。     

Background-free,fast protein staining in sodium dodecyl sulfate polyacrylamide gel using counterion dyes,zincon and ethyl violet

A background-free, fast protein staining method in polyacrylamide gel electrophoresis using an acidic dye, zincon (ZC) and a basic dye, ethyl violet (EV) is described. It is based on the counterion dye staining technique that employs two oppositely charged dyes to form an ion-pair complex in staining solution. The selective binding of free dye molecules to proteins in acidic solution produces bluish violet-colored bands. It is a rapid and end-point staining procedure, involving only fixing and staining steps that are completed in 1-1.5 h. The detection limit of this method is 8-15 ng of protein that is comparable to the sensitivity of the colloidal Coomassie Brilliant Blue G (CBBG) stain. Due to its sensitivity and speed, this stain may be more practical than any other dye-based stains for routine laboratory purposes.     

Modified silver staining in 2DE improves protein detection even at extremely low sample concentration

The typical concentration of protein loaded varies from 0.13 to 1.40 μg/μL for a classical silver staining method in 2DE gel. Here, we present a simple modified classical silver staining method by modifying the silver impregnation and development reaction steps. This modified method detects the protein spots at extremely low loaded concentrations, ranging from 0.0048 to 0.0480 μg/μL. We recommend this modified silver staining as an excellent method for the limited biological samples used for silver‐stained 2DE analysis. Altogether, the protocol takes close to two days from first dimension separation to second dimension separation, followed by silver staining, scanning, and analysis.     

Fluorescent staining of protein in SDS polyacrylamide gels by salicylaldehyde azine

As a noncovalent fluorescence probe, in this study, salicylaldehyde azine (SA) was introduced as a sensitive fluorescence‐based dye for detecting proteins both in 1D and 2D polyacrylamide electrophoresis gels. Down to 0.2 ng of single protein band could be detected within 1 h, which is similar to that of glutaraldehyde‐silver stain, but approximately four times higher than that of SYPRO Ruby fluorescent stain. Furthermore, comparative analysis of the MS compatibility of SA stain with SYPRO Ruby stain indicated that SA stain is compatible with the downstream of protein identification by LC‐MS/MS. Additionally, the probable mechanism of the SA stain was investigated by molecular docking. The results demonstrated that the interaction between SA and protein was mainly contributed by hydrogen bonding and hydrophobic forces.     

鲜红斑痣光动力治疗数学模型及临床验证

为了研究光动力治疗(PDT)中各个因素作用的规律,帮助临床采取有效的治疗方案,针对鲜红斑痣(PWS)组织特性,将光动力治疗中组织光分布、单线态氧产生、光敏剂漂白过程和光敏剂扩散过程结合起来,建立适合于光动力治疗鲜红斑痣病变的系统模型。利用建立的模型,对临床中出现的第二光斑治疗效果差的问题进行仿真研究,发现影响其治疗效果的因素,并通过仿真实验提出改进其治疗效果的新方案。通过临床实验,证明了新方案的有效性和模型的有效性。研究结果说明,针对特定的病例条件建立仿真模型,通过仿真实验可以为临床和理论研究提供一种有效的分析方法。     

光纤光栅传感器温度-应变分离技术的研究

应变、温度分离是影响光纤布喇格光栅(FBG)传感器实际应用的一个重要问题.该文通过分析FBG应变、温度交叉敏感产生的原因,提出了一种基于双FBGs的用于小应变、小温度变化范围的FBG封装.其中一个FBG只感知温度,另一个FBG既受温度的影响也受应变的影响,通过结果计算就可以分离出应变、温度,而且采用温度增敏后还可以提高温度的测试精度.     

超低间隙钛合金低温动态力学性能

本文利用实验应变模态分析方法，在低温条件下（液氦到室温）研究了航天材料超低间隙钛合金ＴＡ７－Ｄ试样的动态力学性能，使用时域系统识别技术分析了材料在不同温度点的模态参数，并根据基频给出了其动态模量随温度的变化。     

细胞凋亡过程中细胞骨架改变的激光共聚焦显微镜观察

目的：了解细胞凋亡过程中细胞骨架的形态学改变。方法：应用高浓度全反式维甲酸诱导卵巢癌细胞凋亡,激光共聚焦显微镜观察经荧光染色的细胞微管蛋白的形态学变化。结果：凋亡早期,微管蛋白在荧光相由正常按一定方向分布的丝网状结构变成杂乱分布的网状结构,晚期呈细颗粒状分布,荧光强度随凋亡发展而逐渐变弱。讨论：在凋亡过程中,细胞骨架随凋亡发展出现相应的形态学改变,凋亡早期,细胞骨架在分布上出现变化,伴随细胞形态出现变化,凋亡晚期,细胞骨架蛋白发生降解,此时的细胞形态改变是不可逆的。     

高温高压固态复分解反应法生长氮化镓的应变性质研究

本文采用高温高压下固态复分解反应法生长氮化镓.用X射线衍射仪、扫描电子显微镜、激光拉曼光谱仪对其进行分析,结果表明生成了六角纤锌矿结构的氮化镓晶体.该样品在宏观上受到了张应力的作用,退火后,宏观的应变状态由张应变向压应变转变;晶体微观应力减小,晶粒尺寸变大,晶体质量变好.