Rapid labeling of amino acid neurotransmitters with a fluorescent thiol in the presence of o‐phthalaldehyde

LIF detection often requires labeling of analytes with fluorophores; and fast fluorescent derivatization is valuable for high‐throughput analysis with flow‐gated CE. Here, we report a fast fluorescein‐labeling scheme for amino acid neurotransmitters, which were then rapidly separated and detected in flow‐gated CE. This scheme was based on the reaction between primary amines and o‐phthalaldehyde in the presence of a fluorescent thiol, 2‐((5‐fluoresceinyl)aminocarbonyl)ethyl mercaptan (FACE‐SH). The short reaction time (<30 s) was suited for on‐line mixing and derivatization that was directly coupled with flow‐gated CE for rapid electrophoretic separation and sensitive LIF detection. To maintain the effective concentration of reactive FACE‐SH, Tris(2‐carboxyethyl)phosphine was added to the derivatization reagents to prevent thiol loss due to oxidation. This labeling scheme was applied to the detection of neurotransmitters by coupling in vitro microdialysis with online derivatization and flow‐gated CE. It is also anticipated that this fluorophore tagging scheme would be valuable for on‐chip labeling of proteins retained on support in SPE.     

pyMolDyn: Identification,structure, and properties of cavities/vacancies in condensed matter and molecules

pyMolDyn is an interactive viewer of atomic systems defined in a unit cell and is particularly useful for crystalline and amorphous materials. It identifies and visualizes cavities (vacancies, voids) in simulation cells corresponding to all seven 3D Bravais lattices, makes no assumptions about cavity shapes, allows for atoms of different size, and locates the cavity centers (the centers of the largest spheres not including an atom center). We define three types of cavity and develop a method based on the split and merge algorithm to calculate all three. The visualization of the cavities uses the marching cubes algorithm. The program allows one to calculate and export pair distribution functions (between atoms and/or cavities), as well as bonding and dihedral angles, cavity volumes and surface areas, and measures of cavity shapes, including asphericity, acylindricity, and relative shape anisotropy. The open source Python program is based on GR framework and GR3 routines and can be used to generate high resolution graphics and videos. © 2016 Wiley Periodicals, Inc.     

分子实验室探针在生化分析中的应用研究进展

分子实验室探针（lab-on-a-molecule probe）是基于分子级别的检测,能够提供空间高密度的感应信号,其主要分为分子逻辑门、分子多模式检测以及兼具2者功能的探针,该探针在多分析物的定性以及竞争分析的定量灵敏检测中发挥了重要的作用。对上述3类探针在生化分析（金属阳离子、无机阴离子、氨基酸、蛋白质、致病菌等）中的应用进行了总结。     

Peptide‐Guided Assembly of Repeat Protein Fragments

Herein, we present the peptide‐guided assembly of complementary fragments of designed armadillo repeat proteins (dArmRPs) to create proteins that bind peptides not only with high affinity but also with good selectivity. We recently demonstrated that complementary N‐ and C‐terminal fragments of dArmRPs form high‐affinity complexes that resemble the structure of the full‐length protein, and that these complexes bind their target peptides. We now demonstrate that dArmRPs can be split such that the fragments assemble only in the presence of a templating peptide, and that fragment mixtures enrich the combination with the highest affinity for this peptide. The enriched fragment combination discriminates single amino acid variations in the target peptide with high specificity. Our results suggest novel opportunities for the generation of new peptide binders by selection from dArmRP fragment mixtures.     

区域立体物流网络规划模型与算法

提出长株潭区域立体物流网络建构及其网络优化设计.精细化定义了模式分担率,构建了更切合实际的双准则双层规划模型.下层规划描述各核心城市物流枢纽间基于多模式多层阶交通条件下的用户选择行为,上层规划追求最小化长株潭区域立体物流网络系统广义物流费用并最大化整个网络的物流运输量,以满足城市群区域经济发展对物流提出的更高要求.给出了可以克服Frank-Wolfe方法缺陷的惩罚Lagrange对偶方法求解下层规划算法,设计了基于实数编码和组合变异的双层规划改进遗传算法,算法可以保证搜索到近似全局最优解.     

On F-almost split sequences

Let A be an Artinian algebra and F an additive subbifunctor of Ext,（-, -） having enough projectives and injectives. We prove that the dualizing subvarieties of mod A closed under F-extensions have F-almost split sequences. Let T be an F-cotilting module in mod A and S a cotilting module over F = End（T）. Then Horn（-, T） induces a duality between F-almost split sequences in ⊥FT and almost sl31it sequences in ⊥S, where addrS = Hom∧（f（F）, T）. Let A be an F-Gorenstein algebra, T a strong F-cotilting module and 0→A→B→C→0 and F-almost split sequence in ⊥FT.If the injective dimension of S as a Г-module is equal to d, then C≌（ΩCM^-dΩ^dDTrA^＊）^＊,where（-）^＊=Hom（g,T）.In addition, if the F-injective dimension of A is equal to d, then A≌ΩMF^-dDΩFop^-d TrC≌ΩCMF^-d ≌F^d DTrC.     

森田六元组的几乎分裂序列

令ΛA_1,Λ_2为两个环,M是(A_2-Λ_1)-双模,且N是(Λ_1-Λ_2)-双模.六元组Γ=(Λ_1,Λ_2,N,M,ψ,φ)是一个森田六元组.对于Γ的表示,确定其几乎分裂序列(也称AR-序列)是非常重要的.通过modΛ_1和modΛ_2的右(左)几乎分裂同态、既约同态构造Γ上的相应同态,并进一步确定它的几乎分裂序列.     

Split‐type octonion matrix

The split and hyperbolic (countercomplex) octonions are eight‐dimensional nonassociative algebras over the real numbers, which are in the form , where e_m's have different properties for them. The main purpose of this paper is to define the split‐type octonion and its matrix whose inputs are split‐type octonions and give some properties for them by using the real quaternions, split, and hyperbolic (countercomplex) octonions. On the other hand, to make some definitions, we present some operations on the split‐type octonions. Also, we show that every split‐type octonions can be represented by 2 × 2 real quaternion matrix and 4 × 4 complex number matrix. The information about the determinants of these matrix representations is also given. Besides, the main features of split‐type octonion matrix concept are given by using properties of  real quaternion matrices. Then, 8n × 8nreal matrix representations of split‐type octonion matrices are shown, and some algebraic structures are examined. Additionally, we introduce real quaternion adjoint matrices of split‐type octonion matrices. Moreover, necessary and sufficient conditions and definitions are given for split‐type octonion matrices to be special split‐type octonion matrices. We describe some special split‐type octonion matrices. Finally, oct‐determinant of split‐type octonion matrices is defined. Definitive and understandable examples of all definitions, theorems, and conclusions were given for a better understanding of all these concepts.     

WEAK AND STRONG CONVERGENCE THEOREMS FOR SPLIT GENERALIZED MIXED EQUILIBRIUM PROBLEM

The purpose of this paper is to introduce a split generalized mixed equilibrium problem (SGMEP) and consider some iterative sequences to find a solution of the generalized mixed equilibrium problem such that its image under a given bounded linear operator is a solution of another generalized mixed equilibrium problem. We obtain some weak and strong convergence theorems.