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111.
The C1–8 fragment of autolytimycin was synthesized via a reliable 10-step route capable of delivering 41% overall yield at multi-gram scale. As a key step, a chelation-controlled isopropenylation of α-oxygenated aldehydes was established with a reagent combination of diisopropenylzinc, magnesium halide, and a dichloromethane/toluene mixed solvent. Cram-chelate isopropenylation products dominated for aldehydes with a small α-substituents, such as –OMe and –OBn groups, while the Felkin product could be obtained with a bulky –OTBS group. 相似文献
112.
Direct Blue 71 staining as a destaining‐free alternative loading control method for Western blotting
Li Zeng Jing Guo Hong‐Bo Xu Rongzhong Huang Weihua Shao Liu Yang Mingju Wang Jianjun Chen Peng Xie 《Electrophoresis》2013,34(15):2234-2239
In Western blotting, a suitable loading control is indispensable for correcting errors in the total amount of loaded protein. Immunodetection of housekeeping proteins and total protein staining have traditionally been used as loading control methods. Direct Blue 71 (DB71) staining—a novel, sensitive, dye‐binding staining method compatible with immunodetection—may offer advantages over these traditional loading control methods. Three common neuroscientific samples (human plasma, human oligodendrocytes, and rat brain) were employed to assess DB71 staining as a loading control method for Western blotting. DB71, CBB, one traditional housekeeping protein, and one protein of interest were comparatively assessed for reliability and repeatability and linear dynamic range over 2.5–40 μg of protein loaded. DB71's effect on the reliability and repeatability and linear dynamic range of immunoreaction were also assessed. Across all three sample types, DB71 was either equivalent or superior to CBB and housekeeping protein‐based methods in terms of reliability and repeatability and linear dynamic range. Across all three sample types, DB71 staining did not impair the reliability and repeatability or linear dynamic range of immunoreaction. Our results demonstrate that the DB71 staining can be used as a destaining‐free alternative loading control method for Western blotting. 相似文献
113.
One of the most challenging problems in dynamic resource allocation for cognitive radio networks is to adjust transmission power of secondary users (SUs) while quality of service needs of both SUs and primary users (PUs) are guaranteed. Most power control algorithms only consider interference temperature constraint in single user scenario while ignoring the interference from PUs to SUs and minimum signal to interference plus noise ratio (SINR) requirement of SUs. In this paper, a distributed power control algorithm without user cooperation is proposed for multiuser underlay CNRs. Specifically, we focus on maximizing total throughput of SUs subject to both maximum allowable transmission power constraint and SINR constraint, as well as interference temperature constraint. To reduce the burden of information exchange and computational complexity, an average interference constraint is proposed. Parameter range and convergence analysis are given for feasible solutions. The resource allocation is transformed into a convex optimization problem, which is solved by using Lagrange dual method. In computer simulations, the effectiveness of our proposed scheme is shown by comparing with distributed constrained power control algorithm and Nash bargaining power control game algorithm. Copyright © 2014 John Wiley & Sons, Ltd. 相似文献
114.
115.
ANDREA BAIOCCHI M. LISTANTI NICOLA BLFARI-MELAZZI C. SOPRANO 《International Journal of Satellite Communications and Networking》1996,14(5):389-412
In this paper, an ATM system architecture for satellite communications is described. The proposed architecture includes an on-board switch and implements the adaptation of real-time services (e.g. voice and video) and non-real-time services (e.g. data) to the satellite communication link, while achieving statistical advantage. To this end, the ATM traffic categories defined in recent specifications1,2 are utilized. Real-time and non-real-time traffic components are simultaneously supported by a TDMA/TDM on the satellite uplink/downlink, respectively. By exploiting the burstiness of real-time traffic, the proposed satellite system architecture achieves a significant increase of the overall system throughput. After describing the satellite system architecture and addressing scheme, we derive analytical models, by using sophisticated queueing models that allow a very accurate performance evaluation and an easy dimensioning of the data buffers. The proposed model is validated with simulations. The main result is the assessed feasibility of on-board buffers with current technology, even under very strict performance requirements on cell loss ratio and for quite high load values (e.g. 80 per cent of the downlink capacity). Finally, a congestion control scheme, based on a combination of preventive and reactive strategies, is proposed and analysed. 相似文献
116.
117.
提出一种小型校园网的组网方案,从逻辑上把校园网分成校务网、教学网、公共网等三个独立的业务网,使用MA5200F作为核心组网设备,使用Quidway○RS2026和Quidway○RS3026做为各逻辑组成的交换机,使用Eudemon 100为专用硬件防火墙设备,并通过S2026的端口的VLAN特性对其用户进行VLAN的划分和访问权限的控制,这使得构建出来的校园网具有性能高、扩展性高、安全性高和管理简单等特点. 相似文献
118.
Zhihua Xie Dimitrios Pavlidis Pablo Salinas Christopher C. Pain Omar K. Matar 《国际流体数值方法杂志》2020,92(7):765-784
A novel control volume finite element method with adaptive anisotropic unstructured meshes is presented for three-dimensional three-phase flows with interfacial tension. The numerical framework consists of a mixed control volume and finite element formulation with a new P1DG-P2 elements (linear discontinuous velocity between elements and quadratic continuous pressure between elements). A “volume of fluid” type method is used for the interface capturing, which is based on compressive control volume advection and second-order finite element methods. A force-balanced continuum surface force model is employed for the interfacial tension on unstructured meshes. The interfacial tension coefficient decomposition method is also used to deal with interfacial tension pairings between different phases. Numerical examples of benchmark tests and the dynamics of three-dimensional three-phase rising bubble, and droplet impact are presented. The results are compared with the analytical solutions and previously published experimental data, demonstrating the capability of the present method. 相似文献
119.
AbstractIn this paper, details of the design work for a tuned vibration absorber to be used on a hollow cylindrical structure is presented. The vibration problem is of resonant type and the tuned vibration absorber is designed to suppress the displacement vibration response of the free end of the slender hollow structure dominated by the contribution of its lowest transverse vibration modes. The structure is modeled using a commercial finite element software. Finite element model of the structure is verified using experimentally obtained frequency response functions and modal parameters. Effective parameters of the tuned vibration absorber design are then determined based on finite element analysis simulations of the vibration suppression performance of the tuned vibration absorber as it is used on the structure. Details of the tuned vibration absorber design are determined and a prototype is fabricated. Prototype tuned vibration absorber is then characterized experimentally both as a standalone system and also as it is used on the main structure. Vibration reduction performance of the physical prototype of the tuned vibration absorber is also compared with its vibration reduction performance estimated from finite element analysis simulations so that the analysis based design process can be validated.Communicated by Dumitru Caruntu. 相似文献
120.
Different living environments of cancer samples lead to different molecular mechanisms of cancer development, which in turn leads to different cancer subtypes. How to identify cancer subtypes is a key issue for the realization of precision medicine. With the development of high-throughput technologies, multi-omics data which can better understand different causes of cancer have emerged. However, the current methods of analyzing cancer subtypes using multi-omics data is mostly derived from population cancer sample data and ignores the differences between different cancer samples. Therefore, the joint analysis of multi-omics based on a single sample may reveal more information about the differences between individual cancers. A strategy for identifying cancer subtypes is proposed based on Single-sample information gain (SSIG) which construct sample feature matrix by considering the heterogeneity of sample. Applying this strategy to current popular subtype identification methods, cancer subtypes can be identified more accurately and the mechanism of cancer can be found from the perspective of a single sample. By comparing different methods in different clustering measure, and using survival analysis, it is shown that SSIG is more suitable for cancer subtype identification than the original multi-omics data, and it is easier to mine the cancer subtype classification mechanism hidden behind the data. 相似文献