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161.
Yifan Xie Ning Qu Shaobin Lin Haojun Jiang Yanyan Zhang Xiuqing Zhang Hao Liang Fang Chen Xueling Ou 《Electrophoresis》2020,41(12):1095-1102
SNPs, combined with massively parallel sequencing technology, have proven applicability in noninvasive prenatal paternity testing (NIPPT) for singleton pregnancies in our previous research, using circulating cell-free DNA in maternal plasma. However, the feasibility of NIPPT in twin pregnancies has remained uncertain. As a pilot study, we developed a practical method to noninvasively determine the paternity of twin pregnancies by maternal plasma DNA sequencing based on a massively parallel sequencing platform. Blood samples were collected from 15 pregnant women (twin pregnancies at 9–18 weeks of gestation). Parental DNA and maternal plasma cell-free DNA were analyzed with custom-designed probes covering 5226 polymorphic SNP loci. A mathematical model for data interpretation was established, including the zygosity determination and paternity index calculations. Each plasma sample was independently tested against the alleged father and 90 unrelated males. As a result, the zygosity in each twin case was correctly determined, prior to paternity analysis. Further, the correct biological father was successfully identified, and the paternity of all 90 unrelated males was excluded in each case. Our study demonstrates that NIPPT can be performed for twin pregnancies. This finding may contribute to development in NIPPT and diagnosis of certain genetic diseases. 相似文献
162.
Massively parallel sequencing (MPS) technologies have the ability to reveal sequence variations within STR alleles as well as their nominal allele lengths, which have traditionally been detected by CE instruments. Recently, Thermo Fisher Scientific has updated the MPS-STR panel, named the Precision ID GlobalFiler next-generation sequencing (NGS) STR Panel version 2, with primers redesigned to add two pentanucleotide tandem repeat loci and profile interpretation supported by the Converge software. Using the Ion Chef System, the Ion S5XL System, and the Converge software, genetic variations were characterized within STR repeat and flanking regions of 30 autosomal STR markers in 115 unrelated individuals from two Chinese population groups (58 Tibetans and 57 Hans). Nineteen STRs demonstrated a relative increase in diversity with the variant sequence alleles compared with those of traditional nominal length alleles. In total, 390 alleles were identified by their sequences compared with 258 alleles that were identified by length. Of these 92 sequence variants found within the STR repeat regions, 40 variants were located in STR flanking regions. Additionally, the agreement of the results with CE data was evaluated, as was the ability of this new MPS panel to analyze case-type (11 samples) and artificially degraded samples (seven samples in triplicate). The results generated from this study illustrate that extensive sequence variation exists in commonly used STR markers in the selected population samples and indicate that this NGS STR panel has the potential to be used as an effective tool for human forensics. 相似文献
163.
164.
In this paper a parallel multigrid finite volume solver for the prediction of steady and unsteady flows in complex geometries is presented. For the handling of the complexity of the geometry and for the parallelization a unified approach connected with the concept of block-structured grids is employed. The parallel implementation is based on grid partitioning with automatic load balancing and follows the message-passing concept, ensuring a high degree of portability. A high numerical efficiency is obtained by a non-linear multigrid method with a pressure correction scheme as smoother. By a number of numerical experiments on various parallel computers the method is investigated with respect to its numerical and parallel efficiency. The results illustrate that the high performance of the underlying sequential multigrid algorithm can largely be retained in the parallel implementation and that the proposed method is well suited for solving complex flow problems on parallel computers with high efficiency. 相似文献
165.
This paper presents a novel parallel processing-based frame synchronous methodology for 155·520 Mbit/s high speed networks according to ITU-T Recommendations G.707, G.708 and G.709. This scheme is expected to relax operating speed requirements of the circuits used in the system. The proposed methodology can be implemented using off-the-shelf low-rate integrated circuits (ICs). The performance of the devised methodology is analysed and found to be similar to that of traditional approaches. Finally, the proposed scheme is efficient and easy to implement at low cost without sacrificing performance. 相似文献
166.
167.
利用梯形公式的余项,将被积函数的二阶导数做幂级数展开,证明了余项是关于求积区间长度的奇数次幂级数.推导出了复合梯形公式的一类渐近展开式,从另一方面印证了Euler-Maclaurin公式. 相似文献
168.
康殿统 《高校应用数学学报(英文版)》2006,21(2):234-242
§1Introductionandmotivation Itisalwaysofinteresttocomparethevariabilityoftworandomvariablesinreliability theoryandeconomics.Thecomparisonsbasedonmomentssuchasvarianceandstandard deviationarenotveryinformativealthoughsuchmeasuresaresimpletouse.Meanwhile,sincethesecomparisonsarestaticones,andhencetheirapplicationsinsomefields,e.g.,reliabilityandfinance,arerestricted.Inthesefields,however,somedynamicalcomparisons areneeded.Asaresult,severalstochasticordersthataremorerefinedhavebeen establishedi… 相似文献
169.
基于两重网格离散和区域分解技巧,提出三种求解非定常Navier-Stokes方程的有限元并行算法.算法的基本思想是在每一时间迭代步,在粗网格上采用Oseen迭代法求解非线性问题,在细网格上分别并行求解Oseen、Newton、Stokes线性问题以校正粗网格解.对于空间变量采用有限元离散,时间变量采用向后Euler格式离散.数值实验验证了算法的有效性. 相似文献
170.
对电压型谐波源提出一种利用变压器和并联谐振抑制谐波的新方法,并通过实验验证了这种方法的有效性. 相似文献