Unraveling the mystery of efficacy in Chinese medicine formula: New approaches and technologies for research on pharmacodynamic substances

Traditional Chinese medicine (TCM) is the key to unlock treasures of Chinese civilization. TCM and its compound play a beneficial role in medical activities to cure diseases, especially in major public health events such as novel coronavirus epidemics across the globe. The chemical composition in Chinese medicine formula is complex and diverse, but their effective substances resemble “mystery boxes”. Revealing their active ingredients and their mechanisms of action has become focal point and difficulty of research for herbalists. Although the existing research methods are numerous and constantly updated iteratively, there is remain a lack of prospective reviews. Hence, this paper provides a comprehensive account of existing new approaches and technologies based on previous studies with an in vitro to in vivo perspective. In addition, the bottlenecks of studies on Chinese medicine formula effective substances are also revealed. Especially, we look ahead to new perspectives, technologies and applications for its future development. This work reviews based on new perspectives to open horizons for the future research. Consequently, herbal compounding pharmaceutical substances study should carry on the essence of TCM while pursuing innovations in the field.     

Solid-phase synthesis of lipidated peptides

A new flexible and efficient methodology for the solid-phase synthesis of lipidated peptides has been developed. The approach is based on the use of previously synthesized building blocks and overcomes the limitations of previously reported methods, since long doubly lipidated peptides can be synthesized by using this route. Furthermore, it was thus possible to prepare a large number of N- and H-Ras peptides bearing a wide range of reporter and/or linking groups--efficient tools for the investigation of biological processes. In terms of efficiency and flexibility this solid-phase method is superior to the solution-phase synthesis. It gives pure peptides in multimilligram amounts within a much shorter time and with superior overall yield.     

Swelling behavior of ionically cross-linked polyampholytic hydrogels in varied salt solutions

Ionically cross-linked polyampholytic hydrogels were synthesized by redox copolymerization of acrylamide and an ionic complex of (N,N-diethylamino)ethyl methacrylate and acrylic acid (designated as PADA hydrogel). The swelling behavior of the hydrogels in water indicated that a minimal equilibrium swelling ratio is found when the molar ratio of anionic/cationic monomers was 1.55. In NaCl solution, the hydrogels exhibited the typical swelling behavior of conventional polyampholytic gels. Their equilibrium swelling ratios increased with an increase in the NaCl concentration. In solutions of multivalent ions (CaCl₂ and trisodium citrate solutions), the equilibrium swelling ratios of the hydrogels increased first and were then followed by a decrease with an increase in salt concentration. Interestingly, an unexpected abrupt swelling phenomenon was observed when the fully swollen hydrogels in salt solution were transmitted to pure water. The unique swelling behavior of PADA hydrogels depends not only on the molar ratio of the anionic/cationic monomers but also on the valency of the ions.     

Calorimetric study of cross-linking polymerization of methyl methacrylate in the presence of a multimonomer

The kinetics of free-radical cross-linking polymerization of methyl methacrylate (MM) in the presence of poly[2-(10-undecenoyloxy)ethyl methacrylate] (PUDEM) as a macromolecular cross-linker has been isothermally examined within the temperature range from 85–100°C using the differential scanning calorimetry (DSC). The activation energy found for this reaction, E _a=89.3 kJ mol^–1, exceeds slightly the literature values of activation energy obtained for the mass polymerization of MM without any cross-linking agent. The activation energy has been also determined by the isoconversion method. It has been found that E _a decreases with the increase in the conversion, which may indicate a change in the reaction mechanism.This work was partly supported by the Committee for Research (KBN) in the framework of project No. 7 T08E 026 20     

Designing a contaminated soil sampling strategy for human health risk assessment

Human health risk assessment is a site-based approach used to identify the potential health hazards which are induced by an old site contamination. For a proper evaluation of the daily doses of contaminants to which people will be exposed given the future occupation of the site, both a characterization and a quantification of soil pollution are needed. Such information can be provided by soil sampling. Thus the choice of the location, the number, depth and type of soil samples is very important and ought to follow a well-defined strategy. A review of contaminated site sampling practices in Europe and North America could not identify any completely formalized sampling strategy for human health risk assessment. On the contrary there are several approaches which can be roughly classified into two categories: a systematic sampling scheme over the whole site, on the one hand, and a sampling design driven by an initial knowledge of the contamination sources and fitted to the suspected pollution pattern, on the other. The first approach provides a complete coverage of the site but it may be rather expensive and entail useless sampling. The performance of the second depends on the quality of prior information. Actually both methods can be combined as explained hereafter. In view of the specificity of each site, the requirements of health risk assessment and the time and cost constraints, it seems difficult to work out a typical soil sampling strategy suitable for all sites. However, some recommendations can be made according to the site dimensions, the nature, degree and heterogeneity of contamination, and the (future) use of the site. The scientist should thus rely on a thorough examination of all available information (site history, geology and hydrogeology, soil properties, contaminants behaviour , etc.) to delimit contaminated areas as homogeneous as possible and then distribute the sampling points (e.g.using a sampling grid). They should also take the potential exposure paths into account in order to define the areas and soil strata to be sampled as a priority. Statistical and geostatistical tools can be helpful for formulating a sampling strategy as well as for interpreting the collected data. Received: 7 December 2001 Accepted: 24 February 2002     

基于“模拟电路”课程开展五环导学法的研究

模拟电路课程在理论性、实践性和工程性等方面的表现均十分突出,是高校学员学习非常吃力的一门课程,如何提高课堂效率,充分利用课前、课中、课后的时间提高学员的理论基础和综合素质,是目前高校模拟电路教学工作中亟待解决的问题。本文以模拟电路课程为依托,深入研究课程内容的特点,构思在课程中开展五环导学法的方法,设计教学活动,并给出实施的策略和方法。     

网络多层语义深度挖掘及流媒体缓存策略研究

对网络音视频数据进行多层次语义的深度挖掘,统计分析用户行为,建立一个流行度预测模型,在此基础上提出智能化的代理缓存替换策略.初步模拟实验表明该策略能提高缓存字节命中率,减短客户端的请求延迟时间.     

电子信息对抗作战对象的未来发展特点及其对抗体系发展战略研究

面对当今世界军事变革、部队转型的新形势,我们必须深刻思考军事战略重心转移所引发的需求变化以及新的武器装备采办政策、技术发展路线图等等问题。从电子信息对抗在未来战争中的作用和地位出发,分析其主体作战对象的发展特点,并根据未来电子信息对抗核心作战能力建设的军事需求,探讨能够确保全谱战斗空间信息优势和决策优势的对抗体系及其发展战略。     

计算机实践教学:问题及对策

任何一个教学计划都涉及到许多方面。有时候某个环节出现较大失误会导致整个计划归于失败。因此必须在重视基础课程课堂授课的基础上，有针对性地、有计划地加强计算机实验课程中基本实验技术的教学，培养学生理论联系实际和良好的动手能力。     

电网故障时双馈风力发电机励磁改进控制策略

双馈风力发电机（DFIG）在风能捕获过程中,传统的定子磁链定向控制都假定电网上的电压恒定不变,定子磁链也恒定不变,没有考虑定子励磁电流的动态响应过程,这个误差在电网故障时会对系统产生极不稳定的作用。为此应考虑该响应过程,对控制檳型进行修正,提出一种应对电网电压跌落时的励磁改迸策略,即在建立转子侧变换器控制模型时,把反映励磁电流过渡过程的定子电压变化量考虑进来。通过推导定子磁场定向下的转子电压方程,搭建定子磁链定向矢量控制模型,经过仿真结果比较,说明在加入励磁补偿电压项后,风电系统在应对电网电压故障时的电流控制性能得到明显改善,有效提升了系统的低电压穿越能力。