Proteomic response and molecular regulatory mechanisms of Bacillus cereus spores under ultrasound treatment

This study was aimed at providing new insights on the proteomic response of bacterial spores to ultrasound. Data-independent-acquisition method was used to quantify the proteome change of Bacillus cereus spores after ultrasound treatment (200 W). This study revealed that 2485 proteins were extracted from Bacillus cereus spores, most of them were related to metabolism. After ultrasound treatment, the expression of 340 proteins were significantly changed (the fold change ≥ 2 and p < 0.05), of which 207 proteins were significantly down-regulated. KEGG pathway analysis showed that differentially expressed proteins mainly distributed in metabolism pathway, cell process pathway and genetic information processing pathway after ultrasound treatment. Furthermore, this study analyzed the differentially expressed proteins in significant enrichment pathways. In particular, the expression of key proteins in the phosphorylation reaction of spores was significantly decreased after ultrasound treatment. Thus, ATP synthesis rate decreased and the phosphorylation reaction inhibited. Also, the decrease of the expression of key proteins related to the tricarboxylic acid cycle led to the decrease of nutrients metabolism of spores. Ultrasound treatment induced the down-regulation of fatty acid synthetase expression and promoted fatty acid metabolism at the same time. The content of fatty acids decreased in spores consequently.     

Ultrafast transverse thermoelectric response in c‐axis inclined epitaxial La0.5Sr0.5CoO3 thin films

Ultrafast transverse thermoelectric voltage response has been observed in c‐axis inclined epitaxial La_0.5Sr_0.5CoO₃thin films. Voltage signals with the rise time of 7 ns have been detected under the irradiation of pulse laser with duration of 28 ns. A concept, named response rate ratio, has been proposed to evaluate the intrinsic response rate, and this ratio in La_0.5Sr_0.5CoO₃is smaller than that in other reported materials. The low resistivity is thought to be responsible for the ultrafast response, as low resistivity induces small optical penetration depth, and response time has a monotonous increasing relationship with this depth.

     

一种高功率共面馈电脉冲辐射天线的设计实验

设计了一种高功率共面馈电脉冲辐射天线。该天线馈源采用双组电大尺寸共面极板馈臂,将馈电点置于焦点处,与直径2.1 m的抛物反射面相配合,实现超宽谱短脉冲的有效定向辐射。提出了一种由同轴输入到4端输出的高功率馈电巴伦,解决了高功率情况下同轴线到4馈臂共面馈电问题。对所设计天线进行测试实验,结果表明：在馈电脉冲宽度为450 ps、峰值电压为142 kV时,辐射因子达到800 kV。     

高功率可变脉宽辐射系统

介绍了一种应用在高功率VHF频段可变脉冲产生及辐射系统的组成。在实验室条件下,通过优化设计、调节实现了数ns至数十ns脉冲宽度辐射输出。利用开路容性天线加载可调电感原理,达到辐射脉冲宽度可变的目的,同时通过并联引入耗能大电感,实现减少辐射脉冲拖尾,增加系统效率的功能;并利用高能量、快脉冲电源为种子电源,为放电回路进行储能放电,利用这一体制减小了系统辐射脉冲间的同步抖动。     

Modulation Characteristics of Tunable Quantum-Well Heterolasers at the Radiation Frequency Tuning

The influence of the tuning of the radiation frequency of quantum-well heterolasers within the limits of their amplification band on the output laser radiation parameters at different frequencies of pump-current modulation has been numerically simulated using two optical models of an active medium under the assumption that the excess of the invariable component of the pump current over its threshold value at any point of the amplification band is constant. It has been established that the amplitude-detuning characteristics of the lasers studied are linear at high current-modulation frequencies and nonlinear at medium ones. The behavior of the nonlinear spectral response of a laser diode is explained by its multiresonance structure and is mainly determined by the ratio between the pump-current modulation frequency and the maximum resonance frequency of the laser. __________ Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 72, No. 4, pp. 473–478, July–August, 2005.     

Asymptotic properties of local polynomial regression with missing data and correlated errors

The main objective of this work is the nonparametric estimation of the regression function with correlated errors when observations are missing in the response variable. Two nonparametric estimators of the regression function are proposed. The asymptotic properties of these estimators are studied; expresions for the bias and the variance are obtained and the joint asymptotic normality is established. A simulation study is also included.     

NONDENSELY DEFINED IMPULSIVE NEUTRAL FUNCTIONAL DIFFERENTIAL INCLUSIONS WITH NONLOCAL CONDITIONS

In this paper,using a fixed point theorem for condensing multi-valued maps,we investigate the existence of integral solutions to a class of nondensely defined neutral evolution impulsive differential inclusions with nonlocal conditions in Banach spaces.     

Measurement and characterization of the human spinal cord SEEP response using event-related spinal fMRI

Although event-related fMRI is able to reliably detect brief changes in brain activity and is now widely used throughout systems and cognitive neuroscience, there have been no previous reports of event-related spinal cord fMRI. This is likely attributable to the various technical challenges associated with spinal fMRI (e.g., imaging a suitable length of the cord, reducing image artifacts from the vertebrae and intervertebral discs, and dealing with physiological noise from spinal cord motion). However, with many of these issues now resolved, the largest remaining impediment for event-related spinal fMRI is a deprived understanding of the spinal cord fMRI signal time course. Therefore, in this study, we used a proton density-weighted HASTE sequence, with functional contrast based on signal enhancement by extravascular water protons (SEEP), and a motion-compensating GLM analysis to (i) characterize the SEEP response function in the human cervical spinal cord and (ii) demonstrate the feasibility of event-related spinal fMRI. This was achieved by applying very brief (1 s) epochs of 22°C thermal stimulation to the palm of the hand and measuring the impulse response function. Our results suggest that the spinal cord SEEP response (time to peak ≈8 s; FWHM ≈4 s; and probably lacking pre- and poststimulus undershoots) is slower than previous estimates of SEEP or BOLD responses in the brain, but faster than previously reported spinal cord BOLD responses. Finally, by detecting and mapping consistent signal-intensity changes within and across subjects, and validating these regions with a block-designed experiment, this study represents the first successful demonstration of event-related spinal fMRI.