基于罗朗级数的矩量匹配技术及其在去耦电容选择中的应用

提出一种新型的矩量匹配技术,不同于传统的基于泰勒级数的矩量匹配,该技术基于罗朗级数展开,考虑了负幂项的作用;通过灵活选择展开阶次,可以在指定区域内或外,非常有效地逼近所给函数.本文针对电源分配系统的去耦电容选择问题,将该型矩量匹配技术应用于这一领域.结果表明,利用该方法可以快速选择合适的电容大小,降低系统的端口阻抗,进而达到优化设计的目的.     

Inorganic Drug‐Delivery Nanovehicle Conjugated with Cancer‐Cell‐Specific Ligand

The surface of layered double hydroxide nanoparticles, a potential drug‐delivery nanovehicle, is modified with the cancer‐cell‐specific ligand, folic acid. The surface modification is successfully accomplished through step‐by‐step coupling reactions with aminopropyltriethoxysilane and 1‐ethyl‐3‐(3‐dimethyl aminopropyl)‐carbodiimide. In order to evaluate the cancer‐cell targeting effect of folic‐acid‐grafted layered double hydroxide utilizing fluorescence‐related assay, both layered double hydroxide with and without folic acid moiety are labeled with fluorescein 5′‐isothiocyanate. The uptake of layered double hydroxide and folic acid conjugated into KB and A549 cells is visualized using fluorescence microscopy and measured by flow cytometry. Both chemical and biological assay results demonstrate that the folic acid molecules are indeed conjugated to the surface of layered double hydroxide and thus the selectivity of nanovehicles to cancer cells overexpressing folate receptors increases. In this study, it is suggested that layered double hydroxide nanoparticles can be used as drug‐delivery carriers with a targeting function due to the chemical conjugation with specific ligand.     

Doxorubicin‐Conjugated Immuno‐Nanoparticles for Intracellular Anticancer Drug Delivery

A polymeric nanoparticle comprised of surface furan groups is used to bind, by Diels–Alder (DA) coupling chemistry, both targeting anti‐human epidermal growth factor receptor 2 (anti‐HER2) antibodies and chemotherapeutic doxorubicin (DOX) for targeted, intracellular delivery of DOX. In this new approach for delivery, where both chemotherapeutic and targeting ligand are attached, for the first time, to the surface of the delivery vehicle, the nuclear localization of DOX in HER2‐overexpressing breast cancer SKBR‐3 cells is demonstrated, as determined by confocal laser scanning microscopy. Flow cytometric analysis shows that the conjugated DOX maintains its biological function and induces similar apoptotic progression in SKBR‐3 cells as free DOX. The viable cell counts of SKBR‐3 cancer cells following incubation with different nanoparticle formulations demonstrates that the combined DOX and anti‐HER2 nanoparticle is more efficacious than the nanoparticle formulation with either DOX or anti‐HER2 alone. While free DOX shows similar cytotoxicity against both cancerous SKBR‐3 cells and healthy HMEC‐1 cells, the combined DOX‐anti‐HER2 nanoparticle is significantly more cytotoxic against SKBR‐3 cells than HMEC‐1 cells, suggesting the benefit of nanoparticle‐conjugated DOX for cell type‐specific targeting. The DOX‐conjugated immuno‐nanoparticle represents an entirely new method for localized co‐delivery of chemotherapeutics and antibodies.     

One‐Step Formulation of Protein Microparticles with Tailored Properties: Hard Templating at Soft Conditions

Formulation of therapeutic proteins into particulate forms is a main strategy for site‐specific and prolonged protein delivery as well as for protection against degradation. Precise control over protein particle size, dispersity, purity, as well as mild preparation conditions and minimal processing steps are highly desirable. It is, however, hard to fit all these criteria with conventional preparation techniques. Here a one‐step hard‐templating synthesis of microparticles composed of functional, non‐denatured protein is reported. The method is based on filling porous CaCO₃ microtemplates with the protein near to its isoelectric point (pI) followed by pH‐ or EDTA‐mediated dissolution of the tempplates. In principle, a wide variety of proteins can be converted into microparticles using this approach. The main requirement is an overlap of the protein insolubility and a template solubility for a certain parameter (here pH or EDTA). Here the formulation of insulin particles is studied in detail and it is shown that particles consisting of high molecular weight protein (catalase) can also be prepared. In this context, the synthesis of CaCO₃ templates with controlled size, the mechanism of the protein microparticle formation and mechanical properties of the microparticles are discussed. For the first time, the fabrication of mesoporous monodispersed CaCO₃ microtemplates with identical porocity but tuned diameter from 3 to 20 μm is demonstrated. The protein particle diameter can be adjusted by choosing the appropriate template size that is critical for successful pulmonary delivery of insulin. As a first step towards insulin delivery, the in vitro release of insulin at physiological conditions is studied.     

神奇的基因芯片

基因芯片运用微电子加工技术以及基因分子的自组装技术在微小芯片上组装成千上万个不同的DNA微阵列 ,实现以基因为主的生命信息的大规模检测。其显著优点在于 :高速、高效、耗费低、便于系统集成和大规模信息的适时处理。基因芯片技术的出现将会给生命科学、医学、化学、新药开发、生物武器战争、司法鉴定、食品和环境卫生监测等领域带来一场革命。本文简要介绍了基因芯片及其应用和近期研究的进展。     

支持WiMAX节点灵活休眠的两阶段可靠多播策略

节能是无线网络的一个重要课题.针对IEEE 802.16e标准第2类节能模型中监听窗口长度固定会导致一些空闲移动站因得不到及时休眠而浪费能量这一不足,该文提出"两阶段可靠多播策略",让基站在第1阶段多播数据包,在第2阶段对第1阶段丢失的数据包进行网络编码并重播.该策略让移动站一旦空闲就进入休眠,实现了时延约束下数据包的可靠传递.仿真试验表明,该策略可以降低能耗,且移动站的占空比、能耗、吞吐率、丢包率等指标均优于传统的重传与确认方案.     

A Charge Reversible Self‐Delivery Chimeric Peptide with Cell Membrane‐Targeting Properties for Enhanced Photodynamic Therapy

The cell membrane is the most important protective barrier in living cells and cell membrane targeted therapy may be a high‐performance therapeutic modality for tumor treatment. Here, a novel charge reversible self‐delivery chimeric peptide C₁₆–PRP–DMA is developed for long‐term cell membrane targeted photodynamic therapy (PDT). The self‐assembled C₁₆–PRP–DMA nanoparticles can effectively target to tumor by enhanced permeability and retention effect without additional carriers. After undergoing charge reverse in acidic tumor microenvironment, C₁₆–PRP–DMA inserts into the tumor cell membrane with a long retention time of more than 14 h, which is very helpful for in vivo applications. It is found that under light irradiation, the reactive oxygen species generated by the inserted C₁₆–PRP–DMA would directly disrupt cell membrane and rapidly induce cell necrosis, which remarkably increases the PDT effect in vitro and in vivo. This novel self‐delivery chimeric peptide with a long‐term cell membrane targeting property provides a new prospect for effective PDT of cancer.     

6‐Mercaptopurine‐Induced Fluorescence Quenching of Monolayer MoS2 Nanodots: Applications to Glutathione Sensing,Cellular Imaging,and Glutathione‐Stimulated Drug Delivery

Molybdenum disulfide (MoS₂) nanodots (NDs) with sulfur vacancies have been demonstrated to be suitable to conjugate thiolated molecules. However, thiol‐induced fluorescence quenching of MoS₂ NDs has been rarely explored. In this study, 6‐mercaptopurine (6‐MP) serves as an efficient quencher for the fluorescence of monolayer MoS₂ (M‐MoS₂) NDs. 6‐MP molecules are chemically adsorbed at the sulfur vacancy sites of the M‐MoS₂ NDs. The formed complexes trigger the efficient fluorescence quenching of the M‐MoS₂ NDs due to acceptor‐excited photoinduced electron transfer. The presence of glutathione (GSH) efficiently triggers the release of 6‐MP from the M‐MoS₂ NDs, thereby switching on the fluorescence of the M‐MoS₂ NDs. Thus, the 6‐MP‐M‐MoS₂ NDs are implemented as a platform for the sensitive and selective detection of GSH in erythrocytes and live cells. Additionally, thiolated doxorubicin (DOX‐SH)‐loaded M‐MoS₂ NDs (DOX‐SH/M‐MoS₂ NDs) serve as GSH‐responsive nanocarriers for DOX‐SH delivery. In vitro studies reveal that the DOX‐SH/M‐MoS₂ NDs exhibit efficient uptake by HeLa cells and greater cytotoxicity than free DOX‐SH and DOX. In vivo study shows that GSH is capable of triggering the release of DOX‐SH from M‐MoS₂ ND‐based nanomaterials in mice. It is revealed that the DOX‐SH/M‐MoS₂ NDs can be implemented for simultaneous drug delivery and fluorescence imaging.     

BPA-Containing Polydopamine Nanoparticles for Boron Neutron Capture Therapy in a U87 Glioma Orthotopic Model

Boron neutron capture therapy (BNCT) is a promising therapy for refractory cancer based on the cytotoxic reaction of ¹⁰B (n, α) ⁷Li. Although two BNCT agents are clinically available, they are quickly metabolized and show modest enrichment in tumor sites, partially limiting BNCT widespread application. Consequently, novel agents that perform active targeting and show good biocompatibility have to be developed. Herein, boronophenylalanine-containing polydopamine (B-PDA) nanoparticles are easily fabricated by encapsulating boronophenylalanine (BPA) in polydopamine via nitrogen-boronate coordination. In this study, B-PDA achieves increased tumor accumulation and prolonged retention effects in the tumor site and superior antitumor activity post neutron irradiation in the orthotopic xenograft glioma model. In brief, this study offers a novel strategy for BPA delivery and may broaden the perspective on nanomedicine design for BNCT.     

随机移动模型下移动自组网无序传输端到端延迟闭解分析

针对移动自组网端到端延迟在封闭形式分析方面的局限性,该文提出一种有效的针对无序传输,单副本两跳中继算法的网络延迟建模方案,并给出其严格的理论延迟上界。首先针对多种随机移动模型,证明了移动节点的相遇间隔时间可归纳为统一表达式。然后,综合分析了媒介竞争、流量竞争、排队延迟等问题,合理划分并精确求解出了各延迟关键时间段,从而构造了数据包排队服务模型。最后推导出移动自组网端到端延迟的封闭形式理论上界。仿真结果表明,该理论延迟与实验数据紧密吻合。