A Hollow‐Core,Magnetic, and Mesoporous Double‐Shell Nanostructure: In Situ Decomposition/Reduction Synthesis,Bioimaging, and Drug‐Delivery Properties

A novel in situ decomposition/reduction approach is developed to manu­facture hollow core, magnetic, and mesoporous double‐shell nanostructures (HMMNSs) via in situ decomposition and reduction of a β‐FeOOH nanorod core and organosilicate‐incorporated silica‐shell precursor. The formed HMMNSs are then aminated by silanization for further covalent conjugation to rhodamine B isothiocyanate (RBITC) and poly(ethylene glycol) (PEG) chains. The resultant RBITC‐grafted and PEGylated nanocomposites (HMMNS–R/Ps) have excellent blood compatibility and very low cytotoxicity towards HeLa and MCF‐7 cells, and can be taken up by cancer cells effectively in a dose‐dependent manner, as confirmed by in vitro flow cytometry, confocal luminescence imaging, and magnetic resonance imaging (MRI) studies. In vivo MRI studies coupled with Prussian blue staining of slides from different organs show that the nanocomposites preferentially accumulate in liver and spleen after intravenous injection, which suggests a potential application of the nanocomposites as MRI contrast agents. Importantly, the HMMNS–R/P nanocomposites show high loading capacity for water‐insoluble anticancer drugs (docetaxel or camptothecin) owing to the presence of a large inner cavity and enhanced surface area and pore volume. Furthermore, the drug‐loaded nanocomposites exhibit greater cytotoxicity than the corresponding free drugs. These results confirm that the HMMNS–R/P nanocomposites are promising candidates for simultaneous bioimaging and drug delivery.     

Effective PEGylation of Iron Oxide Nanoparticles for High Performance In Vivo Cancer Imaging

A practical and effective strategy for synthesizing PEGylated superparamagnetic iron oxide nanoparticles (SPIONs) is established. In this strategy, poly(acrylic acid) (PAA) is combined with SPIONs via multiple coordination between the carboxylic groups of PAA and SPIONs, which introduces abundant carboxylic groups, then, α,ω‐diamino PEG is linked to SPIONs via the amidation of the carboxylic groups. The synthesized PEGylated SPIONs exhibit no cytotoxicity and high resistance to phagocytosis by macrophages in vitro as well as low uptake by the liver and spleen in vivo, which makes the SPIONs highly efficient in tumor imaging by magnetic resonance imaging (MRI) at a relatively low dose of SPIONs. These outstanding properties are largely due to the significant shielding effect of the dense PEG coating as well as the net neutral surface of the PEGylated SPIONs in physiological conditions. In summary, the PEGylated SPIONs prepared by this strategy exhibit great application potential in tumor imaging as MRI contrast agents targeting through enhanced permeability and retention (EPR) effect.     

Design Guidelines For Conjugated Polymers With Light‐Activated Anticancer Activity

Multifunctional materials that simultaneously provide therapeutic action and image the results provide new strategies for the treatment of various diseases. Here, it is shown that water soluble conjugated polymers with a molecular design centered on the polythiophene?porphyrin dyad are effective for killing neighboring cells. Following photoexcitation, energy is efficiently transferred from the polythiophene backbone to the porphyrin units, which readily produce singlet oxygen (¹O₂) that is toxic for the cells. Due to the light‐harvesting ability of the electronically delocalized backbone and the efficient energy transfer amongst optical partners, the polythiophene?porphyrin dyad shows a higher ¹O₂ generation efficiency than a small molecule analog. The fluorescent properties of these polymers can also serve to distinguish amongst living and dead cells. Polymers can be designed with folic acid grafted onto the polymer side chain that can specifically kill folate receptor‐overexpressed cells, thereby providing an important demonstration of anticancer specificity through molecular design.     

岸基双基地雷达ISAR舰船成像时间选择

在建立和分析岸基双基地ISAR舰船目标信号模型的基础上,利用双基地雷达等效雷达视角对舰船目标进行运动分析,并以此为依据选择观测数据段成像,利用RD成像算法得到舰船目标的混合视图;通过回波数据分段成像,利用子图像对舰船目标的多普勒展宽和垂直转动分量进行估计,继而综合这两个估计确定舰船目标的俯视和侧视成像时刻.仿真数据验证...     

FMICW用于单基机载SAR的成像研究

针对调频连续波合成孔径雷达(FMCW SAR)用于单基机载平台时无法实现发射和接收隔离的难题,提出了基于中断连续波(FMICW)的单基机载SAR成像研究。基于FMICW的信号特点,详细讨论了FMICW用于SAR成像时门控脉冲宽度、门控脉冲重复频率、门控脉冲周期数、扫频频率等参数的制约关系,给出了FMICW SAR的系统结构,并有针对性地分析了信号处理模块。最后结合距离多普勒(RD)算法对FMICW SAR进行成像仿真试验,对模型进行了验证。结果表明机载FMICW SAR不但可以有效解决收发隔离,并且可以实现对目标的精确成像。     

基于VC++的多臂井径测井成像软件研究

在工程和生产测井中,为满足油井套管检测资料清晰和直观的需求。详细阐述了如何借助数据库、数字图像处理及面向对象等软件技术,将多臂井径测井仪检测到的套管数据,以曲线及灰度图像方式实时绘制在PC屏幕上。实验结果表明,曲线及图像显示的内容清晰、直观地反映了井下套管情况,给油井的解释和评价打下了良好基础,提高了油井套管检测的效率。     

利用基于扫描相机的荧光寿命成像显微技术研究细胞周期

利用基于扫描相机的荧光寿命成像显微系统,以细胞周期为模型,研究转染绿色荧光蛋白的HeLa细胞的荧光寿命。结果表明,处于周期内不同进程的细胞的荧光寿命为2.50~3.00 ns。处于分裂期的细胞的荧光寿命在1 h内从2.86 ns下降到2.82 ns;在DNA合成前期的8 h内,荧光寿命从2.82 ns下降到2.78 ns。荧光寿命的差异反映了细胞周期中核浆内大分子浓度的变化,对了解细胞周期的分子机制有一定的意义。